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RSS2011 Vol. 32, No. 01
2011, 32(01)
Abstract:
Objective To investigate the anti-inflammatory,analgesic and choleretic effect of Lidan zhitong capsule.Methods The mouse gallbladder was removed after medication and weighed directly to measure the effect of Lidan zhitong capsule on bile secretion and excretion.The test tube dilution method was used to observe the bacteriostatic effect on staphylococcus aureus,alpha hemolytic streptococcus,beta-hemolytic streptococci,shigella dysenteriae,escherichia coli and salmonella.The anti-inflammation and detumescenc...
Objective To investigate the anti-inflammatory,analgesic and choleretic effect of Lidan zhitong capsule.Methods The mouse gallbladder was removed after medication and weighed directly to measure the effect of Lidan zhitong capsule on bile secretion and excretion.The test tube dilution method was used to observe the bacteriostatic effect on staphylococcus aureus,alpha hemolytic streptococcus,beta-hemolytic streptococci,shigella dysenteriae,escherichia coli and salmonella.The anti-inflammation and detumescenc...
2011, 32(01)
Abstract:
Objective To research the whole bone marrow adherence culure condition of mouse bone marrow-derived mesenchymal stem cells,select the best condition,and establish an expansion method of mouse bone marrow-derived mesenchymal stem cells in vitro.Methods We detected the proliferation ability of the bone marrow mesenchymal stem cells in different ages mouse,different concentration of serum and cell density,and found out the optimum culture condition.BMSCs were cultured in the optimum culture condition,and light...
Objective To research the whole bone marrow adherence culure condition of mouse bone marrow-derived mesenchymal stem cells,select the best condition,and establish an expansion method of mouse bone marrow-derived mesenchymal stem cells in vitro.Methods We detected the proliferation ability of the bone marrow mesenchymal stem cells in different ages mouse,different concentration of serum and cell density,and found out the optimum culture condition.BMSCs were cultured in the optimum culture condition,and light...
2011, 32(01)
Abstract:
Objective To isolate and identify brain derived neurotrophic factor(BDNF)in the cerebral cortex of adult Rhesus monkey,and investigate its histological distribution.Methods The cortex tissues of monkeys were harvested,homogenized and isolated by SDS PAGE electrophoresis,and identified by western blot using rabbit BDNF specific antibody.Then the frozen sections from cortex were prepared,and the immunohistochemistry method was used to detect the distribution of BDNF in the cerebral cortex of adult Rhesus monk...
Objective To isolate and identify brain derived neurotrophic factor(BDNF)in the cerebral cortex of adult Rhesus monkey,and investigate its histological distribution.Methods The cortex tissues of monkeys were harvested,homogenized and isolated by SDS PAGE electrophoresis,and identified by western blot using rabbit BDNF specific antibody.Then the frozen sections from cortex were prepared,and the immunohistochemistry method was used to detect the distribution of BDNF in the cerebral cortex of adult Rhesus monk...
2011, 32(01)
Abstract:
Objective To investigate the effect of PNS in regulating the expression of Cathepsin B in rabbits with pulmonary fibrosis of pulmonary heart disease from the perspective of pathology.Methods Forty healthy Japanese rabbits were randomly divided into five groups(eight rabbits in each group):the control group,the PF model group,the PNS prevention group,treatment group and the western medicine treatment group.The expression of CB protein and the pathological structural changes in lung tissue were observed under...
Objective To investigate the effect of PNS in regulating the expression of Cathepsin B in rabbits with pulmonary fibrosis of pulmonary heart disease from the perspective of pathology.Methods Forty healthy Japanese rabbits were randomly divided into five groups(eight rabbits in each group):the control group,the PF model group,the PNS prevention group,treatment group and the western medicine treatment group.The expression of CB protein and the pathological structural changes in lung tissue were observed under...
2011, 32(01)
Abstract:
Objective To establish an useful method to isolate and culture umbilical cord mesenchymal stem cells(UC-MSCs)and induce it to neural differentiation.Methods Thirty human umbilical cords(UCs) were obtained from pregnant women with their written consent and the approval of the Clinic Ethnics Committee,the primary UC-MSCs were isloated by adherent culture in the medium contains 20% fetal bovine serum(FBS),then they were maintained in the medium contains 10% FBS and induced to neural cells in neural differentia...
Objective To establish an useful method to isolate and culture umbilical cord mesenchymal stem cells(UC-MSCs)and induce it to neural differentiation.Methods Thirty human umbilical cords(UCs) were obtained from pregnant women with their written consent and the approval of the Clinic Ethnics Committee,the primary UC-MSCs were isloated by adherent culture in the medium contains 20% fetal bovine serum(FBS),then they were maintained in the medium contains 10% FBS and induced to neural cells in neural differentia...
2011, 32(01)
Abstract:
Objective To discuss the correlation between micro-inflammation state and cardiovascular disease(CVD)in patients with maintenance hemodialysis(MHD).Method The various inflammatory factors and cardiovascular complications were observed and analyzed in patients maintenance hemodialysis(MHD).Results The level of various inflammatory factors in MHD group were significantly higher than those in non-hemodialysis group(P<0.05),and the various inflammatory factors in MHD group were correlated to cardiovascular comp...
Objective To discuss the correlation between micro-inflammation state and cardiovascular disease(CVD)in patients with maintenance hemodialysis(MHD).Method The various inflammatory factors and cardiovascular complications were observed and analyzed in patients maintenance hemodialysis(MHD).Results The level of various inflammatory factors in MHD group were significantly higher than those in non-hemodialysis group(P<0.05),and the various inflammatory factors in MHD group were correlated to cardiovascular comp...
2011, 32(01)
Abstract:
Objective To investigate the effect of vacuum sealing drainage(VSD)technology on infectious abdominal wound surface.Method Six patients with infectious wound surface after abdominal injury who were treated by vacuum sealing drainage,were retrospectively studied in our department from October 2007 to September 2009.Results In all patients,wound surface healed very well,the frequency of change dressings obviously decreased,the healing time of infected raw surface and postoperative hospital day shortened,the c...
Objective To investigate the effect of vacuum sealing drainage(VSD)technology on infectious abdominal wound surface.Method Six patients with infectious wound surface after abdominal injury who were treated by vacuum sealing drainage,were retrospectively studied in our department from October 2007 to September 2009.Results In all patients,wound surface healed very well,the frequency of change dressings obviously decreased,the healing time of infected raw surface and postoperative hospital day shortened,the c...
2011, 32(01)
Abstract:
Objective To investigate the clinical effect of YADRAN herbal tooth powder combined with periodental non-surgical treatment on periodontitis.Methods 115 patients with chronic periodontitis were selected and divided randomly into two groups:the experimental group(57)and the control group(58).Patients in the experimental group used YADRAN herbal tooth powder and patients in the control group used normal toothpaste to brush their teeth after periodental non-surgical treatment.We observed and compared their cli...
Objective To investigate the clinical effect of YADRAN herbal tooth powder combined with periodental non-surgical treatment on periodontitis.Methods 115 patients with chronic periodontitis were selected and divided randomly into two groups:the experimental group(57)and the control group(58).Patients in the experimental group used YADRAN herbal tooth powder and patients in the control group used normal toothpaste to brush their teeth after periodental non-surgical treatment.We observed and compared their cli...
2011, 32(01)
Abstract:
Objective To discuss the diagnosis and treatment of focal nodular hyperplasia of the liver(FNH).Method Retrospective analysis was made on 12 FNH cases in terms of clinical findings,images,pathologic examination.Results The average age of patients with FNH was 33 years old.Symptoms were helpless for diagnosis of FNH.The correct diagnostic rate of BUS,CT and MRI was 25%,44.44% and 50%,respectively.All 12 cases underwent operation got uneventful recovery.Conclusions CT and MRI are mandatory for the diagnosis o...
Objective To discuss the diagnosis and treatment of focal nodular hyperplasia of the liver(FNH).Method Retrospective analysis was made on 12 FNH cases in terms of clinical findings,images,pathologic examination.Results The average age of patients with FNH was 33 years old.Symptoms were helpless for diagnosis of FNH.The correct diagnostic rate of BUS,CT and MRI was 25%,44.44% and 50%,respectively.All 12 cases underwent operation got uneventful recovery.Conclusions CT and MRI are mandatory for the diagnosis o...
2011, 32(01)
Abstract:
Objective To study the relationship between periphery blood T lymphocytes subgroup and the stage of rectal adenocarcinoma in patients who could be cured surgically.Method CD3,CD4,CD8,CD4/CD8,natural killer(NK) cells and B cells of 87 patients with preoperative rectal adenocarcinoma and 40 controls were tested by flow ctyometry.Results The expression of CD8 and NK cell of patients in preoperative rectal adenocarcinoma group was significantly higher than those in the control group(P<0.05);the expression of CD...
Objective To study the relationship between periphery blood T lymphocytes subgroup and the stage of rectal adenocarcinoma in patients who could be cured surgically.Method CD3,CD4,CD8,CD4/CD8,natural killer(NK) cells and B cells of 87 patients with preoperative rectal adenocarcinoma and 40 controls were tested by flow ctyometry.Results The expression of CD8 and NK cell of patients in preoperative rectal adenocarcinoma group was significantly higher than those in the control group(P<0.05);the expression of CD...
2011, 32(01)
Abstract:
Objective To investigate the value of 16-slice spiral CT multiplanar reconstruction in diagnosis of orbital blowout fracture.Method 389 patients with orbital blowout fracture were given axial scan and multiplanar reconstruction(MPR) by 16-slice spiral CT,and the axial scan MPR results were compared.Results 23 cases of the fracture of lower orbital wall were misdiagnosed from axial imaging.There is no misdiagnosis case from coronal imaging.The fracture of lower orbital wall and bone fracture shifting were di...
Objective To investigate the value of 16-slice spiral CT multiplanar reconstruction in diagnosis of orbital blowout fracture.Method 389 patients with orbital blowout fracture were given axial scan and multiplanar reconstruction(MPR) by 16-slice spiral CT,and the axial scan MPR results were compared.Results 23 cases of the fracture of lower orbital wall were misdiagnosed from axial imaging.There is no misdiagnosis case from coronal imaging.The fracture of lower orbital wall and bone fracture shifting were di...
2011, 32(01)
Abstract:
Objective To observe the therapeutic effect of combined therapy on patients with gastroparesis after major abdominal surgery.Methods Fifteen patients with gastroparesis after major abdominal surgery were prohibited for food and water,and were given gastrointestinal decompression and drug treatment.The therapeutic effect was observed 3 weeks after treatment.Results Fourteen patients with gastroparesis after abdominal surgery recovered completely in 3 weeks,and one patient recovered in the 5th week,the gastri...
Objective To observe the therapeutic effect of combined therapy on patients with gastroparesis after major abdominal surgery.Methods Fifteen patients with gastroparesis after major abdominal surgery were prohibited for food and water,and were given gastrointestinal decompression and drug treatment.The therapeutic effect was observed 3 weeks after treatment.Results Fourteen patients with gastroparesis after abdominal surgery recovered completely in 3 weeks,and one patient recovered in the 5th week,the gastri...
2011, 32(01)
Abstract:
Objective To study the early diagnostic value of multi-slice spiral CT perfusion imaging (CTP)I in acute cerebral ischemia.Methods Conventional and perfusion CT scanning were performed in fifty patients suspected acute ischemia with symptom onset within one and 12 hours.CT and clinical follow up were obtained in all patients in 3~7 days after the first time of CT examination.Results In the first conventional CT scanning,21 patients were detected lesions of infarction,29 patients had negative results,but in ...
Objective To study the early diagnostic value of multi-slice spiral CT perfusion imaging (CTP)I in acute cerebral ischemia.Methods Conventional and perfusion CT scanning were performed in fifty patients suspected acute ischemia with symptom onset within one and 12 hours.CT and clinical follow up were obtained in all patients in 3~7 days after the first time of CT examination.Results In the first conventional CT scanning,21 patients were detected lesions of infarction,29 patients had negative results,but in ...
2011, 32(01)
Abstract:
Objective To explore the incidence rate and the risk factors of postoperative delirium in the young and the middle-aged elective noncardiac surgical patients.Methods A cluster sampling method was adopted in this cross-sectional study.1 276 patients,aged from18 to 50,undergoing elective noncardiac surgery were recruited from March to June,2010.The patients with delirum or diagnosed as dementia,depression,and cognitive dysfunction before operation were ruled out in this study.The enrolled patients were interv...
Objective To explore the incidence rate and the risk factors of postoperative delirium in the young and the middle-aged elective noncardiac surgical patients.Methods A cluster sampling method was adopted in this cross-sectional study.1 276 patients,aged from18 to 50,undergoing elective noncardiac surgery were recruited from March to June,2010.The patients with delirum or diagnosed as dementia,depression,and cognitive dysfunction before operation were ruled out in this study.The enrolled patients were interv...
2011, 32(01)
Abstract:
Objective To study X-ray radiographic and CT findings in patients with severe A(H1N1) virus infection.Method We reviewed the chest X-ray radiographic and CT findings of 24 patients with confrmed severe A(H1N1)virus infection in our hospital from November 2009 to December 2009.Results(1)The initial radiograph was normal in 4 patients among 24 patients with severe A(H1N1)virus infection.(2)Chest radiography and CT found ground-glass opacity in the lungs accounted for 53% and 67%,and mixed ground-glass opacity...
Objective To study X-ray radiographic and CT findings in patients with severe A(H1N1) virus infection.Method We reviewed the chest X-ray radiographic and CT findings of 24 patients with confrmed severe A(H1N1)virus infection in our hospital from November 2009 to December 2009.Results(1)The initial radiograph was normal in 4 patients among 24 patients with severe A(H1N1)virus infection.(2)Chest radiography and CT found ground-glass opacity in the lungs accounted for 53% and 67%,and mixed ground-glass opacity...
2011, 32(01)
Abstract:
Objective To observe the cytotoxic activity in vitro of cytokine-induced killer cells(CIK cells),and to observe the short-term clinical efficacy,immunological activity and side effects of CIK cells in treating genitourinary system tumor.Methods A total of 10 patients with genitourinary system malignancies were recorded with CIK cell therapy from July 2007 to March 2010.Peripheral blood mononuclear cells(PBMC)from patients with standard treatment protocols were induced to CIK cells in vitro,then phenotype of...
Objective To observe the cytotoxic activity in vitro of cytokine-induced killer cells(CIK cells),and to observe the short-term clinical efficacy,immunological activity and side effects of CIK cells in treating genitourinary system tumor.Methods A total of 10 patients with genitourinary system malignancies were recorded with CIK cell therapy from July 2007 to March 2010.Peripheral blood mononuclear cells(PBMC)from patients with standard treatment protocols were induced to CIK cells in vitro,then phenotype of...
2011, 32(01)
Abstract:
Objective To discuss the procedures and techniques of pleural decortication in the treatment of patients with chronic empyema.Method The clinical data of 73 patients with chronic empyema who received pleural decortication from June 2005 to June 2010 were retrospectively analyzed.Result All the 73 cases obtained satisfying results on operation time,volume of blood loss and postoperative recovery.Conclusion Pleural decortication with some techniques can get better results in the treatment of chronic empyema.
Objective To discuss the procedures and techniques of pleural decortication in the treatment of patients with chronic empyema.Method The clinical data of 73 patients with chronic empyema who received pleural decortication from June 2005 to June 2010 were retrospectively analyzed.Result All the 73 cases obtained satisfying results on operation time,volume of blood loss and postoperative recovery.Conclusion Pleural decortication with some techniques can get better results in the treatment of chronic empyema.
2011, 32(01)
Abstract:
Objective To investigate the clinical effects of hepatic arterial embolism with lipiodol and Bleomycin A5 emulgator on hepatic haemangioma.Method After femoral arteriopuncture,the selective catheterization in hepatic artery was performed at the feeding artery of hepatic haemangioma,then the hepatic arterial embolism with lipiodol and Bleomycin A5 emulgator was given to treat 51 patients with hepatic haemangioma.Results 51 patients were given CT examination 10 days after hepatic arterial embolism,the deposit...
Objective To investigate the clinical effects of hepatic arterial embolism with lipiodol and Bleomycin A5 emulgator on hepatic haemangioma.Method After femoral arteriopuncture,the selective catheterization in hepatic artery was performed at the feeding artery of hepatic haemangioma,then the hepatic arterial embolism with lipiodol and Bleomycin A5 emulgator was given to treat 51 patients with hepatic haemangioma.Results 51 patients were given CT examination 10 days after hepatic arterial embolism,the deposit...
2011, 32(01)
Abstract:
Objective To explore the reason and lesson of misdiagnosis of mesentery panniculitis as colonic tumor.Method The clinical data of four patients with mesenteric panniculitis were retrospectivey analyzed.Result Four cases were misdiagnosised as colonic tumor before operation.Conclusions The diagnosis rate of mesenteric panniculitis is low before operation,and the differential diagnosis is complex.Clinical Surgeon should attach importance to mesenteric panniculitis.
Objective To explore the reason and lesson of misdiagnosis of mesentery panniculitis as colonic tumor.Method The clinical data of four patients with mesenteric panniculitis were retrospectivey analyzed.Result Four cases were misdiagnosised as colonic tumor before operation.Conclusions The diagnosis rate of mesenteric panniculitis is low before operation,and the differential diagnosis is complex.Clinical Surgeon should attach importance to mesenteric panniculitis.
