2011 Vol. 32, No. 05

2011, 32(05)
Abstract(1501)
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A Preliminary Study on Magnetic Resonance Diffusion Imaging of Normal Canine Spinal Cord
2011, 32(05)
Abstract(1995)
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Objective To explore the feasibility,technical features and research significance of magnetic resonance diffusion-weighted imaging(DWI)of normal canine spinal cord.Methods Eight beagle dogs were anesthetized with Sumianxin,then their spinal cord diffusion imaging was performed immediately.Siemens sonata1.5T MRI System was used to scan with different parameters on the basis of echo-planar imaging(EPI).The image characteristics was analyzed and compared among these dogs,and their average apparent diffusion co...
Protective Effects of 20(R)-ginsenoside Rg3 against PAF-induced Injury of Human Umbilical Vein Endothelial Cells
2011, 32(05)
Abstract(2215)
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Objective To investigate the protective effect of 20(R)-ginsenoside Rg3 against PAF-induced human umbilical vein endothelial cell(HUVEC)injury and its preliminary mechanism.Methods Platelet activating factor(PAF)was used to injure HUVEC in vitro.Cell viability was assessed with methyl thiazolyl tetrazolium(MTT)assay.The cultured cells were loaded by Fura-2/AM and the change of i in HUVEC was measured by fluorospectrophotometry.The levels of tissue plasminogen activator(t-PA)and plasminogen activator inhibit...
Protective Effects of Baicalin Against Learing and Memory Capacity of Mice with Cerebral Ischemia-reperfusion Injury
2011, 32(05)
Abstract(2105)
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Objective To investigate the protective effect and mechinasm of baicalin against the learning and memory capacity of mice with cerebral ischemia-reperfusion injury.Methods Sixty Kunming mice were randomly divided into three groups:control group(n=20),reperfusion group(n=20)and baicalin group(n=20).Water maze of Morris was used to detect the learning and memory capacity,and latent period and swimming locus were recorded.Western blot analysis was used to detect Bax and Bcl-2 protein expression in mice in each...
Identification and Culture of Hippocampus Neurons in Fetal Rats
2011, 32(05)
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Objective To establish the protocol for culturing hippocampus neurons in vitro and explore the neuronal growth character istics.Methods Tissues from hippocampus of fatal rats were harvested,then digested into cell suspension by using 0.125% trypsin.After planted into wells for 2 hours,cell suspension was transferred into next well for continuing incubation.The cell growth was observed at day 5,11 and 15.Neurons specific Enolase(NSE)antibody,recognized specifically NSE antigen in cultured cells,was used to i...
Simultaneous Observation of Blood Pressure and Microcirculation of Rabbits with Acute Hemorrhagic Shock after Treatment
2011, 32(05)
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Objective To improve the knowledge of students on the occurrence,the development,and the treatment of the hemorrhagic shock.through monitoring changes in microcirculation.Methods In the classic hemorrhagic shock experiment,the blood pressure and respiration of the animal were observed.Meanwhile,the mesenteric microcirculation was monitored and recorded in the whole course.The restoring effects on blood pressure and respirattion,especially,on restoration of the microcirculation by means of four treatments we...
The Expression Significance and Relationship Study of SDF-1 and VEGF in Glioma
2011, 32(05)
Abstract(1917)
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Objective To detect the expression of SDF-l and VEGF protein.and investigate their relationship and significance in glioma.Method We detected the expresion of SDF-l and VEGF in glioma by using IHC in 32 cases,8 cases in each level(I-IV).Results The expression of SDF-1 and VEGF was correlated with tumor grade.Positive correlation between SDF-l and VEGF was found in glioma.Conclusions The expression of SDF-1 and VEGF may has synergetic effect and promote the occurrence and development of glioma.Combined deter...
Influence of Hypoxia on Hepatoma Carcinoma Cell Line SMMC7721 in Vitro
2011, 32(05)
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Objective To investigate the effect of hypoxia on human hepatoma carcinoma cell line SMMC7721 cultured in vitro.and to explore the possible molecular mechanism.Methods The chemical method using COCl2 to induce hypoxia environment of SMMC7721 cell was performed.The effect of hypoxia on cell growth,cycle and apoptosis,and adhesion and migration ability were detected by MTT assay,flow cytometry,cell adhesion test,and transwell chamber methods,respectively.RT-PCR and immunocytochemistry methods were used to det...
The Cause Analysis on the Failure of Tumor Cells Culture from Malignant Hydrothorax
2011, 32(05)
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Objective Obtaining primary cells is a very important procedure in sensitivity of chemotherapeutics in vitro and immunotherapy.This article is to reveal need advertent thing in culturing primary tumor cells by analyzing the culture failure cause,so as to select right culture method and avoid failure in the future.Method The cells of sediment from malignant hydrothorax were vaccinated at RPMI Medium 1640 in aseptic condition to observe cell growing.Results The many fibroblasts grew in fifteen collection samp...
Chromosomal Analysis in Spontaneous Abortion during the First Trimester after Assisted Reproduction Technique Treatment
2011, 32(05)
Abstract(2604)
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Objective To know about chromosome abnormalities in spontaneous abortion during the first trimester after assisted reproduction technique treatment and to provide some data for eugenics.Methods A retrospective analysis was performed on 41 clinical spontaneous abortion cases during the first trimester following ART,which including in vitro fertilization-embryo transfer(IVF-ET)and intracytoplasmic sperm injection(ICSI)and frozen embryo transfer(F-ET)and intrauterine insemination(IUI).Cytogenetic analysis of t...
Effects of Exercise Training on Glucose Intolerance in Rats with Type 2 Diabetes Induced by Alloxan
2011, 32(05)
Abstract(2197)
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Objective To analyze the effects of exercise training on glucose intolerance in rats with type 2 diabetes induced by alloxan.Methods Male Wistar rats aged 5 days were injected with either 250 mg/kg of body weight of alloxan or citrate buffer 0.01 M(cortrols).After weaning,half of the animals in each group were subjected to physical training adjusted to meet the aerobic-anaerobic metabolic transition by swimming 1 h/day for 5 days a week with weight overloads.When aged 28,90,and 120 days,the rats underwent g...
Two-dimensional Strain Echocardiography to Assess Left Ventricular Systolic Function in Patients with Metabolic Syndrome
2011, 32(05)
Abstract(1917)
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Objective To assess left ventricular systolic function in patients with metabolic syndrome by two-dimensional strain echocardiography.Methods 24 patients with MS and non-LVH(left ventricular hypertrophy,MS-NLVH group),26 patients with MS and LVH(MS-LVH group)and 28 healthy subjects(control group)were included.The two-dimensional loop-cinec was obtained in apical 4-chamber view,apical 2-chamber view and long axis of left ventricle(LV)view.Eighteen segments of LV longitudinal peak systolic strain and global p...
Analysis of Clinical Significance in 32 Patients with Miller-Fisher Syndrome
2011, 32(05)
Abstract(1099)
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Objective To Analysis the clinical significance of Miller-Fisher syndrome to intend to find a way to increase the identification rate of diagnosis.Methods The clinical materials from 32 patients with Miller-Fisher syndrome was reported by means of retrospective analysis.Results Among the 32 cases,there are preliminary symptoms in 29 cases,typical triad in 19 cases,and the phenomenon of protein-cell separation in all cases.Moreover,immunity-globin increases in 28 cases,and is very effective in the treatment ...
The Clinical Analysis of Cushing's Syndrome-Related Osteoporosis
2011, 32(05)
Abstract(2552)
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Objective To improve the knowledge of Cushing's syndrome and reduce the misdiagnosis rate,we investigated the clinical manifestation of Cushing's Syndrome with primary symptom of osteoporosis characterized by bone pain and fractures.Methods 28 patients with Cushing's syndrome in recent 10 years were enrolled in this study.Their primary symptom and major clinical characteristics were summarized.The bone mineral density of lumbar vertebrae and femur neck was measured by dual-energy X-ray absorptiometry to ana...
Diagnosis and Treatment of 26 Patients with Occupying Lesion in Duodenal Papilla
2011, 32(05)
Abstract(2442)
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Objective To the explore the clinical characteristics of duodenal papilla cancer,so as to improve the diagnostic levels and make early diagnosis for duodenal papilla cancer.Methods The clinical data of the 26 patients with occupying lesion in duodenal papilla were analyzed retrospectively in order to summarize the clinical characteristics and diagnosis and treatment experiences of occupying lesion in duodenal papilla.Results Adenocarcinoma was the most common tumor in occupying lesion in duodenal papilla,ac...
The Variation of Quantitative Lung Data on Vibration Response Imaging System
2011, 32(05)
Abstract(2393)
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Objective To explore the application of Quantitative Lung Data(QLD)of VRIxpTM Vibration Response Imaging System(VRI)in diagnosis of pulmonary diseases.Methods From Oct 1,2009 to Sep 1,2010,there were 212 patients with identified diagnosis and examined by VRI.The data of QLD were analyzed retrospectively.Among these patients,there were 59 patients with pneumonia,114 patients with COPD,17 patients with pulmonary tumor,13 patients with pleural effusion,9 patients with pneumothorax.The descriptive statistics an...
Correlation Study of GABABR1 Gene Polymorphism with OSHAS Accompanied by Hypertension
2011, 32(05)
Abstract(1594)
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Objective To investigate the correlation of GABABR1 Phe658Phe gene polymorphism with OSAHS accompanied by hypertension.Methods Twenty-four severe OSAHS patients(12 with normal blood pressureand 12 with hypertension),12 essential hypertension patients and 12 normal control subjects were involved in this study,the polymerase chain reaction-restriction fragment length polymorphism was used to investigate the Phe658Phe polymorphism of GABABR1 gene.Results There was no significant difference in the genotypes and...
Diagnosis and Treatment of 11 Patients with Malignant Peritoneal Mesothelioma
2011, 32(05)
Abstract(2302)
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Objective To discuss the cause,clinical manifestation and treatment of peritoneal mesothelioma.Method The clinical symptoms,surgery status,surgical approach and treatment of 11 patients with pathologically proved malignant peritoneal mesothelioma(MPM)were retrospectively analysed.Result Operative findings and pathological examination were the primary means for diagnosis of MPM.Conclusion Surgery and chemotherapy is an effective way to comprehensive treatment of MPM.
The Analysis and Influence of Anterior Preperitoneal Space on Chronic Pain after Herniorrhaphy
2011, 32(05)
Abstract(1909)
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Objective To investigate the influence of anterior preperitoneal space on chronic pain after herniorrhaphy.Methods Retrospective study was used to analyze the experience of tension-free repair in 368 patients in Kunming Yan'an Hospital from December 2007 to December 2009.175 patients received PHS procedure and 193 patients received Rutkow procedure.They are followed-up to know whether they have postoperative chronic pain.Result The incidence of chronic pain after herniorrhaphy was 3.4%(6/175)and 9.3%(18/193...
Retrospective Analysis on Glucolipid Metabolism of 64 Patients with Liver Cirrhosis after Treatment with Recombinant Growth Hormone
2011, 32(05)
Abstract(1769)
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Objective To retrospectively analyze the glucolipid metabolism in patients with liver cirrhosis after treatment with recombinant growth hormone.Methods We analyzed the triglycerides(TG),serum total cholesterol(TCH),fasting blood sugar(FBS),serum insulin(FINS)and insulin resistance index(IRS)in 64 patients with liver cirrhosis after treatment with recombinant growth hormone from January 2007 to June 2009.Results There was no statistically significant difference in each test index of patients with liver cirrh...
Clinical Trial of Ganmaoxiaoyan Tablets in the Treatment of Influenza A(H1N1)Infection in Children
2011, 32(05)
Abstract(1763)
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Objective To observe efficacy and safety of traditional Chinese medicine in Yunnan-GanMaoXiaoYan tablets in treatment of children with influenza A(H1N1)infection.Methods According to the case inclusion criteria,we collected influenza A(H1N1)suspected cases and randomly divided them into treatment group and control group.Patients in treatment group and control group were given treatment drug and control drug,respectively.We detailedly recorded the clinical information,collected nasal swabs before and after t...
The Clinical Study on Treatment of Acute Cerebral Infarction with Hydrochloric Fasudil
2011, 32(05)
Abstract(2306)
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Objective To investigate the clinical efficacy and safety of fasudil in the treatment of acute cerebral infarction.Method A randomized study was designed to evaluate effect of fasudil on acute cerebral infarction,with routine therapy as placebo 2 control.120 patients were chosen from October 2009 to October 2010 and were divided into 2 groups:fasudil group(60 cases)and control group(60 cases).Excellency,efficacy and improvement of neurological function were observed.Result There was a significant difference...
Therapeutic Strategies of Solitary Kideney and Staghorn Stone(Report of 52 Cases)
2011, 32(05)
Abstract(2044)
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Objective To explore the therapeutic strategies of solitary kideney and staghorn stone and then to summarize the experience.Methods 52 patients,aged from 21 to 63,with solitary kidney and staghorn stone,were treated from May,2006 to November,2008.Surgical methods,operation time,the rates of free stone and the changes of renal function before and after operation were analyzed.Results All cases were treated successfully without gross bleeding,infectious shock and death.Among them,39 were via PCNL,13 via open ...
Clinical Application of Saphenous Island Flap in Treatment of Soft Tissue Defect in Distal Calf, Foot or Ankle
2011, 32(05)
Abstract(1891)
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Objective To summarize the clinical application of saphenous island flap in treatment of soft tissue defects of distal calf,foot or ankle.Method Surgical outcome of 11 cases of soft tissue defects in distal calf,foot or ankle which were treated by saphenous island flap were retrospectively analyzed.Results Good coverage of the defects was achieved except partial necrosis of flap edge in one case.The limbs seem to be slightly bulging after surgery but had good function.Conclusions The major advantages of sap...
The Experience of Minimally Invasive Surgery in Diagnosis and Treatment of Lung Benign Tumor
2011, 32(05)
Abstract(1228)
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Objective To investigate the application value of minimally invasive surgery in the diagnosis and treatment of lung benign tumor.Methods From July 2007 to December 2010,73 patients with lung benign tumor were enrolled in this study.69 times(same bilateral 3 times)of thoracoscope surgery were performed,including VATS and VAMT(video-assisted mini-thoracotomy),and 72 tumors were removed.Surgical methods:lung wedge resection in 22 patients(including bilateral VATS in 3 patients),lobectomy resection in 11 patien...
A Case-control Study on the Risk Factors of Maternal Mortality in Rural Areas of Yunnan Province
2011, 32(05)
Abstract(1962)
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Objective To investigate the risk factors of maternal mortality in rural areas of Yunnan Provience,so as to supply some recommendations to reduce the maternal mortality.Methods A 1:2 case-control study was adopted,the Self-designed questionnaire survey was conducted face to face in somebody's house.Results Under the economical factors been controlled,Logistic Regression Analysis showed that the age of diliver was older(OR=1.109),women with no prenatal care(OR=9.165),cesarean section(OR=4.883)and distance wa...
Comparison of Effects between Two Kinds of Air Disinfection Methods in Consecutively Occupied Heart Interventional Operating Room
2011, 32(05)
Abstract(1883)
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Objective To compare the efficacy of ultraviolet rays and dynamic air purifying machine for air disinfection in the consecutively occupied heart interventional operating room.Methods Different air disinfection methods were performed in heart interventional operating room.Sampling and bacteria counting were taken before sterilization,after-sterilization of the first operation and before the continuous operations,30 min,60 min,120 min during the continuous operations.Results Two methods had good disinfection ...
Cause Analysis and Strategies of Catheter-related Infections in Peripherally Inserted Central Catheter
2011, 32(05)
Abstract(2487)
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Objective To study the cause and strategies of catheter-related infections in malignancy patients who carried a PICC(Peripherally Inserted Central Catheter) for a long time.Methods From October 2008 to December 2010,there were 160 patients who carried a PICC.We analyzed their clinical information to find the cause of catheter-related infections.Results Catheter-related infections occurred in 5 patients(2.9%)of 160 patients.We made correspondent nursing strategies for them according to the causes.Conclusion ...
Teaching Reform of Postgraduate Curriculum about Software Endnote
2011, 32(05)
Abstract(1682)
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Objective To investigate the significance and the teaching reform of postgraduate curriculum about software Endnote.Method The fundamental usage about software Endnote was firstly instructed,and then postgraduates practically operated by themselves combining with teacher's guide.Result All postgraduates can understand the basic usage about software Endnote during shorter period.Conclusions The theory and the practice can be combined together by this teaching method,which is understood easily by postgraduate...
A Survey on the Cognition and Needs of Medical Students to General Practice Education in a Medical College
2011, 32(05)
Abstract(2026)
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Objective To understand the cognition and needs of medical students to the general practice education,and provide the reference for establish the efficient general practice education system.Methods Through stratified random sampling,students were inquired with the constructive questionnaire.All data were recorded in Epidata 3.1 and analyzed with SPSS13.0.Result 81.9% inquired students never get the general practice education;Most of the students have not enough knowledge,They have high needs for the general...
Overall Quality Evaluation from National 108 Employers on Graduates Training in Kunming Medical College
2011, 32(05)
Abstract(1132)
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To understand the overall quality of the current problems on graduates' training in Kunming Medical College,the cross-sectional questionnaires were posted to the national 108 employers,including companies or hospitals our graduates worked in China.Through the analysis of the questionnaire,the overall quality of graduate students in our college reached to the intermediate level at least.Practice is the only criterion for testing truth,medicine is a practical science,therefore,continuing to strengthen the ide...
Effects Evaluation of Participatory Teaching and Traditional Teaching in Information Literacy Education
2011, 32(05)
Abstract(2475)
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Objective To evaluate the effectiveness of participatory teaching and traditional teaching in information literacy education.Methods Two classes of college students were assigned randomly to be taught with participatory teaching methods(experimental class)and traditional teaching methods(control class) respectively.Scores of assignments,computer-based practice test and written examination were compared for the effects evaluation of the two teaching methods.Results The difference in the scores of assignment ...
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