体外诱导猪骨髓间充质干细胞向尿路上皮细胞分化的实验研究
Inducing Differentiation of Pig's Bone Mesenchymal Stem Cell(BMSC) to Urothelial Epithelia in Vitro
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摘要: 目的探讨体外诱导骨髓间充质干细胞(BMSCs)向膀胱尿路上皮细胞分化的可行性研究,为组织工程膀胱的建立提供一种新的种子细胞来源.方法抽取猪的骨髓液在体外行全血原代培养、扩增及鉴定骨髓间充质干细胞.用机械分离法获取膀胱尿路上皮组织,对其行原代培养、扩增、鉴定并提取尿路上皮细胞培养液.选取第三、四代的BMSCs和尿路上皮细胞分成四组:A组:BMSCs+尿路上皮细胞+表皮细胞生长因子;B组:BMSCs+尿路上皮细胞条件培养液+表皮细胞生长因子;C组:BMSCs+表皮细胞生长因子;D组:BMSCs 2 mL.结果用流式细胞仪检测BMSC表面特异阳性标志物CD29、CD44,阴性标志物CD45,结果均为阳性.用角蛋白AE1/AE3单克隆抗体鉴定尿路上皮细胞及实验A、B组结果均为阳性,C、D组均为阴性.结论骨髓间充质干细胞具有多向分化的能力,模拟体内的微环境可使BMSC向尿路上皮细胞分化.Abstract: Objectives To discuss the feasibility of inducing differentiating BMSC(Bone Mesenchymal Stem Cell)to urothelial epithelia in vitro,and to look for a new source of seed cell for the form of bladder in tissue engineering.Methods We extracted the pig's bone marrow in vitro,then primarily cultured them,and enpanded and identified BMSCs.We obtained the urothelial epithelium tissue by mechanical separation,and primarily cultured,amplified and identified the urothelial epithelium,and collected the culture supernat...
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