脑压板牵拉作用对局部脑代谢变化影响的微透析实验研究
Effect of Brain Retraction on Local Cerebral Metabolism: Experimental Study of Microdialysis
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摘要: 目的 研究脑牵拉作用对局部脑组织生化代谢变化的影响,探讨微透析技术在监测脑牵拉伤局部脑组织生化代谢改变中应用潜力.方法 根据不同的牵拉压力(20 mmHg、30 mmHg、40 mmHg)、不同的牵拉方式(间断牵拉和持续牵拉)对脑组织进行牵拉,应用微透析技术动态收集细胞外液(ECF)透析液.观察[Glu]d、[Lac]d、[Gly]d、[Gluta]d和L/P比值的变化.结果 不同的脑牵拉压力引起了不同程度的ECF中[Glu]d、[Lac]d、[Gly]d、[Gluta]d和L/P比值改变.各牵拉组之间进行比较并与对照组及损伤前相比,差异有统计学意义(P<0.05).牵拉压力越大,变化越显著.持续牵拉脑组织比间断牵拉脑组织产生了更为严重的脑损害.持续牵拉组ECF中[Glu]d、[Lac]d、[Gly]d、[Gluta]d、L/P比值变化更明显,各组比较差异有统计学意义(P<0.05).结论 微透析技术是一种较为理想的动态监测脑损伤局部生化改变的方法,可以作为在神经外科手术中监测牵拉性脑损伤产生的一种有价值的工具.Abstract: Objective To study the effect of brain retraction on local biochemical metabolism in brain tissue and to investigate the potential application of microdialysis in monitoring local cerebral metabolic and biochemical changes caused by brain retraction injury.Methods Depending on different traction pressure(20 mmHg,30 mmHg,40 mmHg)and different traction mode(intermittent and continuous traction),the brain tissues were tracted.Microdialysis was applied for the dynamic collection of extracellular fluid(ECF)of di...
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Key words:
- Brain retraction /
- Glucose /
- Lactic acid /
- Glycerol /
- Biochemical metabolism /
- Microdialysis /
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