结核感染T细胞斑点试验在HIV/AIDS合并结核感染诊断中的应用
Clinical Application of T-SPOT.TB in Diagnosis of HIV/AIDS and TB Patients
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摘要: 目的探讨结核感染T细胞斑点试验(T-SPOT.TB)在临床诊断HIV/AIDS合并结核感染的应用价值.方法利用T-SPOT.TB方法,测定60例疑似HIV/AIDS合并结核感染患者,30例健康体检者,20例非结核疾病患者外周血单个核细胞中结核感染特异的效应T淋巴细胞的频率.结果 60例疑似合并结核患者,最终确诊结核35例,其中26例结核抗原特异ELISPOT阳性,提示该诊断方法敏感度为74.3%,30名健康对照中,4名健康体检阳性,20例非结核疾病患者均阴性,该方法的特异性为86.7%.结论结核感染T细胞斑点试验是较灵敏和特异的快速检测结核分枝杆菌感染的方法,可用于HIV/AIDS合并结核感染的辅助诊断.Abstract: Objective To evaluate the clinical application of T-SPOT.TB(enzyme-linked immunospot assay in detection of Mycobacterium tuberculosis infection)in diagnosis of HIV/AIDS and TB patients.Methods We used the T-SPOT.TB to detect T cells with sectetion of IFN-r specific for M.tuberculosis antigents in blood samples from 60 HIV/AIDS patients suspected with tuberculosis,30 healthy persons and 20 patients with other diseases.Results Among 60 HIV/AIDS patients suspected with tuberculosis,35 cases were diagnosed with...
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Key words:
- AIDS /
- TB /
- Diagnosis /
- Tuberculosis /
- Immunoenzyme techniques
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