云南宣威肺腺癌原代细胞和肺腺癌细胞株XWLC-05体外形态学比较
Comparison of the Morphological Changes between Xuanwei Primary Lung Cancer Cells and Lung Adenocarcinoma Cell Line XWLC-05 in vitro
-
摘要: 目的比较宣威肺腺癌原代细胞和已建立的宣威肺腺癌细胞株XWLC-05的形态学变化.方法用含10%胎牛血清的RPMI Medium1640培养基培养宣威肺腺癌细胞和XWLC-05,观察两者的形态学变化,比较不同时间点肺癌细胞数量变化,探讨其生长特性.结果培养的肺癌原代细胞为贴壁成团片状生长,为多边形或类圆形,异型性明显;而XWLC-05细胞成翼状,多边形,增殖速度比本实验室培养肺癌原代细胞快.结论肺癌原代细胞形态学上不同于XWLC-05,有其自身生长特性.Abstract: Objective To compare the morphological changes between the Xuanwei lung cancer primary cells and established Xuanwei lung adenocarcinoma cell line XWLC-05.Methods Xuanwei lung adenocarcinoma cells and XWLC-05 cell line were cultured with 10% fetal bovine serum RPMI Medium 1640,and the growth characteristics of them were explored through observing the morphological changes and comparing the number of lung cancer cells at different time points.Results The primary lung cancer cells cultured in our laboratory g...
-
Key words:
- Lung cancer /
- Primary culture /
- XWLC-05 /
- Comparative morphology
点击查看大图
计量
- 文章访问数: 2127
- HTML全文浏览量: 769
- PDF下载量: 33
- 被引次数: 0