摘要:
目的建立犬的颅脑外伤模型,运用微透析技术研究脑创伤后局部脑代谢的变化.方法取家犬9只,随机分为轻度创伤组(n=3),重度创伤组(n=3)和对照组(n=3).以自由落体硬膜外撞击方法制作轻度和重度脑损伤模型,应用微透析技术动态收集脑损伤局部的脑组织细胞外液(extracellular fluid,ECF),检测颅脑创伤状态下葡萄糖(Glucose,Glu)、乳酸(Lactic Acid,Lac)、甘油(Glycerol,Gly)含量及其动态变化.结果犬脑损伤后Glu含量降低,伤后5.5 h,6 h变化最显著(P<0.01),之后缓慢上升;Lac含量在伤后出现与Glu相反的变化,伤后5.5 h上升至最高值(P<0.05);重度组尤为甚.Gly在伤后6 h缓慢上升,但其变化不明显(P>0.05).结论微透析技术监测脑损伤后ECF中Glu和Lac含量的变化,可以作为判断伤情和预后的重要生化指标.
Abstract:
Objective To establish the traumatic brain injury(TBI)model in canis,and monitor the variations of topical biochemical substances by intracranial microdialysis.Methods Nine healthy adult male mongrel canines were randonmized into three groups:mild TBI group(n=3),severe TBI group(n=3),and control group(n=3).The model was established by the method of free falling body extradural hitting.The extracellular fluid(ECF)was collected by using the intracranial microdialysis.Then the content variations of Glucose,Lac...
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