MAPKs在硫化氢抗大鼠肢体缺血再灌注所致肺损伤中的作用
Role of MAPKs in Protection of Hydrogen Sulfide against Lung Injury Following Limb Ischemia-reperfusion in Rats
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摘要: 目的观察丝裂原活化蛋白激酶(MAPKs)在外源性硫化氢(H2S)抗大鼠肢体缺血再灌注(IR)所致肺损伤中的作用.方法健康SD大鼠,随机分为四组(每组n=8):对照组(Control),Control+NaHS组,IR组和IR+NaHS组.应用双大腿根部止血带复制大鼠双后肢缺血及再灌注后肺损伤模型.Control和IR组动物分别于再灌注前10 min和相应的对照时间点腹腔注射无菌生理盐水(0.5 mL/kg);IR+NaHS和Control+NaHS组动物分别于再灌注前10 min和相应的对照时间点腹腔注射NaHS(28μmol/kg);观察大鼠肺组织学、肺组织中中性粒细胞(PMN)数目、肺组织湿重和干重之比(W/D)、丙二醛(MDA)含量以及动物生存情况等变化.应用Westernblotting检测肺组织中三种磷酸化MAPKs-细胞外信号调节激酶(ERK)、c-Jun氨基末端激酶(JNK)和p38表达的变化.结果与Contorl组相比,IR组动物死亡率、肺组织PMN数目、W/D、MDA含量以及磷酸化ERK、JNK和p38表达均显著增高(P<0.05);与IR组相比,IR+NaHS组动物死亡率、肺组织中PMN数...Abstract: Objective To observe the role of mitogen-activated protein kinases(MAPKs)in protection of exogenous hydrogen sulfide(H2S)against lung injury induced by ischemia-reperfusion(IR)of hind limbs in rats.Methods A rat model of limb ischemia was made by tourniquets placed on both hind limbs at a site proximal to trochanter major and the lung injury was induced following reperfusion.Adult male Spraguce-Dawley(SD)rats were randomly divided into four groups(n=8 per group):Control,Control+NaHS,IR and IR+ NaHS.The rats...
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Key words:
- Mitogen-activated protein kinase /
- Hydrogen sulfide /
- Reperfusion injury /
- Limbs /
- Lung
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