Abstract: ［Abstract］Objectives　To find the the association of Nogo and estrogen receptor gene polymorphism with multiple sclerosis．Methods（1）The blood samples prepared from 47 MS patients and 47 normal person who were matched in age，sex and ethnic were used in this study.（2）To identify the Nogo 3 UTR CAA insertion/deletion polymorphism，polymerase chain reaction（PCR）and polyacrylamide gel electrophoresis were employed. （3）To explore PvuⅡand XbaI restriction fragment length polylmorphism of estrogen receptor（ER） gene， polymerase chain reaction-restriction fragment length polylmorphism（PCR-RFLP）method was used.（4） Data analysis was performed using SPSS．Results　Significant difference in Nogo 3 UTR CAA insertion/deletion allele frequencies was observed between the patients with multiple sclerosis and control （P＜0.05）．The gene polymorphism of Nogo and ER were also analyses using Logistics Regression， the results showed that ER PvuⅡ p allele was negatively associated with MS， the correlation coefficient was -1.670（P＝0.013）with 95% confidence interval between 0.050 and 0.704. And Nogo Insert alletic gene was also negative associatied with MS，correlation coefficient was -1.248（P＝0.013）with 95% confidence interval between 0.087 and 0.948．Conclusions　This study confirms an association of Nogo 3 UTR CAA insertion/deletion polymorphism and PvuⅡrestriction fragment length polylmorphism of estrogen receptor（ER）gene with multiple sclerosis．In addition，ER PvuⅡ p and Nogo Insert alletic gene may be protection factors for MS.