云南省2009年至2014年B型流感毒株血凝素基因进化分析
Phylogenetic Analysis of Influenza B Hemagglutinin Gene in Yunnan from 2009 to 2014
-
摘要: 目的 了解云南省2009年至2014年B型流感病毒血凝素(HA1)基因突变及其抗原变异情况.方法将云南省2009年至2014年流感哨点监测医院采集的流感样病例咽拭子进行病毒分离,随机抽取12株B型流感病毒进行HA1基因序列测定,并对测序产物进行分析,通过MEGA软件进行同源性比对、构建HA基因进化树.结果 云南省流感毒株血清型和基因型鉴定有差异,可能是由于基因变异后量的积累量不够引起.在HA1蛋白的抗原决定簇的3个重要区域120环,150环和160环都有氨基酸的变异.云南省流感毒株在120环的131位置氨基酸变异为N131K,137位置B-Victoria系氨基酸发生了N137H的变异,187位置有2株发生了P187S的变异.结论 云南省的B型流感毒株在血凝素基因重要位置发生了多处变异,有和疫苗株相同的变异,也有不同于疫苗株的变异.Abstract: Objective To investigate the mutation of hemagglutinin gene and amino acid variation of influenza B. Methods Influenza B virus was isolated from throat swab samples in sentinel surveillance of Yunnan province from 2009 to 2014. HA1 gene sequence analysis was applied to determine 12 randomly-selected strains of influenza B virus. The results were analyzed,MEGA software was used to do homology comparison and HA gene phylogenetic tree was established.Results Differences on the serotype and genotype identification of influenza strains were found and it might be caused by inadequate gene mutation accumulation. Amino acid variations were found in 3 important regions of antigenic determinants in HA1 protein: ring 120, ring 150 and ring 160. The amino acid variation of position 131 in ring 120 was N131 K, and in position 137 was N137 H. Two strains had P187 S mutation in position 187. Conclusion There are some important variations in the hemagglutinin gene of influenza B strains in Yunnan Province,with some variations being the same as vaccine strains and some being not.
-
Key words:
- Influenza B /
- Hemagglutinin /
- HA1gene /
- Amino acid variation /
- Phylogenetic analysis
-
[1] [1]KAPRANOS N,PETRAKOUE,ANASTASIADOU C,et al.Detection of herpessimplex virus,cytomegalovirus,and Epstein-Barr virus in thesemen of men attending an infertility clinic[J].Fertil Steril,2003,79(suppl3):1566-1570. [2]于娟,李红饶华祥,等.青海省2010-2012年H3亚型流感病毒HA1基因特性研究[J].中国病原生物学杂志,2013,(10):889-892. [3]金奇.医学分子病毒学[M].北京:科学出版社,2001:2-3. [4]金青青,茅海燕,孙逸,等.乙型流感病毒VIctoria系和Yamagata系HA1基因的分子进化研究[J].中华流行病杂志,2013,34(4):366-370. [5]曾晓旭,王大燕,舒跃龙.B型流行性感冒病毒基因进化研究进展[J].中华预防医学杂志,2013,47(7):656-658. [6] [6]WANG Q,CHENG F,LU M,et al.Crystal structure of unligandedinfluenza B virus hemagglutinin[J].Virology,2008,82(6):3011-3020. [7]陈继明,郭元吉,吴昆昱,郭俊峰乙型流行性感冒病毒两大谱系的起源及其演变特征[J].病毒学报,2001,17(4):322-327. [8] [8]ROTA P A,WALL IS T R,HARMON M W,et al.Cocirculation of two distinct evolutionary lineages of influenza type B virus since1983[J].Virology,1990,175(1):59-68. [9]方斌,刘琳琳,李翔,等.2010-2015年湖北省乙型流感病毒流行和进化分析[J].疾病监测,2016,31(7):554-560. [10] 金青青,茅海燕,孙逸,等.乙型流感病毒Victoria系和Yamagata系[J].HAl基因的分子进化研究中华流行病学,2013,34(4):366-370. [11]鲁俊鹏,周荣,欧志英,等.B型流感病毒广州株的分离及血凝素基因进化分析[J].广东医学,2008,29(7):1120-1122. [12] [12]MCCULLERS J A,WANG G C.Reassortment and insertion deletion are strategies f or the evolution of influenza B Viruses in nature[J].Virology,1999,73(9):7343-7348. -
点击查看大图
计量
- 文章访问数: 1542
- HTML全文浏览量: 581
- PDF下载量: 56
- 被引次数: 0
下载:
