丹参水提物对小鼠病理脱发模型毛发的再生影响
Effect of Water Extract from Salvia Miltiorrhiza on the Hair Regrowth in Testosterone-induced Alopecia in Mice
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摘要: 目的 研究丹参水提物对小鼠病理脱发模型毛发再生的促进作用.方法 睾酮诱导建立小鼠脱发模型,分为空白组,模型组,章光101组,丹参低、中、高剂量组(20、40、80 mg/m L)组.涂药21 d,测量脱毛区毛长、毛重和毛囊数,检测血清睾酮(T)、雌二醇(E2)含量,脱毛区皮肤组织血管内皮生长因子(VEGF)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)含量.结果 (1)丹参水提物能明显促进小鼠再生毛发的毛长、毛重和毛囊数(P<0.05);(2)降低T和提高E2含量(P<0.05),平衡激素水平;(3)提高SOD和GSH-Px活性,降低MDA含量(P<0.05);(4)提高VEGF含量(P<0.05).结论 在睾酮诱导的小鼠病理脱发模型中,丹参水提物能有效促进毛发的再生,其作用机制可能与平衡激素水平、抗氧化和促进VEGF表达有关.Abstract: Objective To study the role of water exact from salvia miltiorrhiza(SM) in promoting hair regrowth in testosterone(T)-induced alopecia in mice. Methods Pathological alopecia mice were induced by T and the mice were randomly divided into control group, model group, Zhanggvanp 101 group, SM low-dose group,SM middle-dose group,SM high-dose group(20, 40, 80 mg/ml, respectively). The effects of SM on hair regrowth were evaluated by measuring hair length, weight and the number of hair follicle. The concentration of serum T and estrodiol(E2), the content of skin VEGF, MDA, SOD, and GSH-Px were measured after external inunctum for 21 days. Results(1) SM significantly promoted regrowth hair length, weight and the number of hair follicle(P<0.05).(2) SM significantly balanced the hormones by reducing levels of T and increasing E2(P<0.05).(3) SM significantly reduced the content of MDA by increasing the activity of SOD and GSH-Px(P<0.05).(4) SM significantly increased the level of VEGF(P<0.05). Conclusion In the pathological alopecia mice induced by T, SM can effectively promote the hair regrowth, with the possible mechanism of balancing hormones, being anti-oxidant and improving VEGF expression.
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[1]廖金凤,段西凌.雄激素性脱发的病因病机[J].临床合理用药,2013,6(5):174-176. [2] [2]YIP,RUFAUT N,SINCLAIR R.Role of genetics and sex steroid hormones in male androgenetic alopecia and female pattern hair loss:an update of what we now know[J].Australas J Dematol,2011,52(2):81-88. [3]韩丽,黄娟,杨秀梅,等.丹参水溶性成分丹酚酸B减压提取工艺研究[J].中华中医药杂志,2013,28(11):3201-3203. [4]苏彦宏,段为钢,李惠兰.滇产复方甘西鼠尾草酸注射液对大鼠心肌缺血再灌注心脏功能的影响[J].昆明医科大学学报,2015,36(2):15-33. [5]尹东锋,陈效.丹参水取物对小鼠耐缺氧作用的影响[J].中国医药导报,2012,9(6):33-35. [6]陈瑶,王胜娟,李朝玲,等.丹参对于急性微循环障碍大鼠血管内皮细胞的保护作用[J].陕西中医,2016,37(4):506-508. [7]周茹,何耀,和丽芬,等.丹参水提物对大鼠心肌缺血-再灌注损伤氧化应激的影响[J].2014,30(2):76-78. [8]李春梅,杨日福,顾取良,等.丹参水提取物对去卵巢大鼠血脂和肝脏的影响[J].华南理工大学学报,2012,40(12):151-156. [9]周乐,崔燎,吴铁,等.丹参水提物预防糖皮质激素对大鼠睾丸损害的实验研究[J].广西医学,2013,35(3):275-277. [10] [10]ZHOU L,ZUO Z,CHOW MS.Danshen:an overview of its chemistry,pharmacology,pharmacokinetics,and clinical use[J].Journal of Clinical Pharmacology,2005,45(12):1345-1359. [11]哈斯其美格.中药“舒发康”治疗脂溢性脱发的实验研究[J].西北民族大学学报,2009,30(4):61-64. [12]沈璐,陈科力.中医药治疗脱发的研究与分析[J].中南民族大学学报,2011,30(1):42-45. [13]刘向慧,王琪,宋丽雅,等.膺嘴豆素A对病理脱发模型小鼠毛发生长影响的初步研究[J].天然产物研究与开发,2014,26(4):613-618. [14]王熠,翟燕芳,刘军平,等.性激素水平与老年慢性心衰竭患者心功能的关系[J].昆明医科大学学报,2015,36(10):16-21. [15] [15]MURATA K,NOGUCHI K,KONDO M,et al.Inhibitory activities of Puerariae Flos against testosterone 5α-reductase and its hair growth promotion activities[J].Journal of Natural Medicines,2012,66:158-165. [16]贾肖肖,宋秀祖.自噬在皮肤氧化应激和色素代谢中的作用[J].中西医结合皮肤性病学杂志,2016,15(3):194-196. [17]张栩,徐锦雄,李锦鸣.中药复方ZDS对烧伤大鼠门静脉血清SOD、MDA的影响[J].昆明医科大学学报,2014,35(2):5-7. [18]洪旭华,陈凯.结合药联合利福喷丁对肺结核患者免疫功能、炎症因子、VEGF及MMP-9的影响[J].昆明医科大学学报,2016,37(2):111-114. [19]于乐,丁虹.男性原发性骨质疏松患者VEGF及HIF与骨代谢指标相关性分析[J].昆明医科大学学报,2014,35(9):61-63. -
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