丹参水提物对小鼠病理脱发模型毛发的再生影响
Effect of Water Extract from Salvia Miltiorrhiza on the Hair Regrowth in Testosterone-induced Alopecia in Mice
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摘要: 目的 研究丹参水提物对小鼠病理脱发模型毛发再生的促进作用.方法 睾酮诱导建立小鼠脱发模型,分为空白组,模型组,章光101组,丹参低、中、高剂量组(20、40、80 mg/m L)组.涂药21 d,测量脱毛区毛长、毛重和毛囊数,检测血清睾酮(T)、雌二醇(E2)含量,脱毛区皮肤组织血管内皮生长因子(VEGF)、丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)含量.结果 (1)丹参水提物能明显促进小鼠再生毛发的毛长、毛重和毛囊数(P<0.05);(2)降低T和提高E2含量(P<0.05),平衡激素水平;(3)提高SOD和GSH-Px活性,降低MDA含量(P<0.05);(4)提高VEGF含量(P<0.05).结论 在睾酮诱导的小鼠病理脱发模型中,丹参水提物能有效促进毛发的再生,其作用机制可能与平衡激素水平、抗氧化和促进VEGF表达有关.Abstract: Objective To study the role of water exact from salvia miltiorrhiza(SM) in promoting hair regrowth in testosterone(T)-induced alopecia in mice. Methods Pathological alopecia mice were induced by T and the mice were randomly divided into control group, model group, Zhanggvanp 101 group, SM low-dose group,SM middle-dose group,SM high-dose group(20, 40, 80 mg/ml, respectively). The effects of SM on hair regrowth were evaluated by measuring hair length, weight and the number of hair follicle. The concentration of serum T and estrodiol(E2), the content of skin VEGF, MDA, SOD, and GSH-Px were measured after external inunctum for 21 days. Results(1) SM significantly promoted regrowth hair length, weight and the number of hair follicle(P<0.05).(2) SM significantly balanced the hormones by reducing levels of T and increasing E2(P<0.05).(3) SM significantly reduced the content of MDA by increasing the activity of SOD and GSH-Px(P<0.05).(4) SM significantly increased the level of VEGF(P<0.05). Conclusion In the pathological alopecia mice induced by T, SM can effectively promote the hair regrowth, with the possible mechanism of balancing hormones, being anti-oxidant and improving VEGF expression.
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