NO/iNOS与关节软骨破坏的相关性

唐涛; 孙先润; 姜艳萍; 张敏; 马骅; 龙宇

NO/iNOS与关节软骨破坏的相关性

唐涛^1,2,
孙先润^1,3,
姜艳萍⁴,
张敏¹,
马骅¹,
龙宇¹

1. 云南省第一人民医院康复医学科
2. 昆明理工大学医学院
3. 云南大学
4. 昆明医科大学校医院

基金项目:

基金：云南省应用基础研究(昆医联合专项)基金资助项目(2015FB095); 云南省应用基础研究计划项目(2013FZ182);

计量
- 文章访问数: 2363
- HTML全文浏览量: 857
- PDF下载量: 86
- 被引次数: 11
出版历程
- 收稿日期: 2016-05-24

Correlation Between NO/iNOS and Destruction of Articular Cartilage

Tang Tao ^1,2,
Sun Xian Run ^1,3,
Jiang Yan Ping ⁴,
Zhang Min ¹,
Ma Hua ¹,
Long Yu ¹

Funds:

基金：云南省应用基础研究(昆医联合专项)基金资助项目(2015FB095); 云南省应用基础研究计划项目(2013FZ182);

摘要

摘要: 目的探讨关节软骨破坏与一氧化氮/诱导型一氧化氮合成酶(NO/i NOS)动态变化关系.方法40只健康成年Wistar雄性大鼠随机分为对照组(自由活动)和实验组(过度运动训练).于0、1、2、3、6周时采集大鼠全膝关节,4%PFA液固定、石蜡包埋、切片、染色;通过Safranin-O染色观察软骨形态学变化,ELISA法测定血清/关节液中NO/i NOS含量变化,Mankin's评分,统计并分析软骨病理学改变.结果过度运动训练能导致关节软骨从细胞排序紊乱、基质丢失到表面毛糙、裂缝等系列软骨破坏和退变病理改变过程;过度运动训练能使大鼠血清、关节液中NO/i NOS的含量增加,血清NO/i NOS组:1周(48.088±1.534),(3.058±0.073);2周(54.574±0.800),(6.630±0.524);3周(68.020±3.184),(7.384±0.306);6周(104.394±8.094),(11.364±0.882).关节液NO/i NOS组:1周(65.814±1.610),(8.126±0.724);2周为(72.766±2.344),(9.694±0.564);3周(88.816±0.819),(11.326±1.473);6周为(120.204±12.044),(15.166±1.198),并随运动量递增,保持较高浓度水平.结论关节液或血清中NO/i NOS含量变化与关节软骨的破坏程度呈正向关系.
- 一氧化氮 /
- 诱导型一氧化氮合成酶 /
- 关节软骨 /
- 骨关节炎
Abstract: Objective To investigate the dynamic changes of articular cartilage destruction and nitric oxide nitric oxide/inducible nitric oxide synthase(NO/i NOS). Methods Forty healthy adult male Wistar rats were randomly divided into control group(free movement) and experimental group(overtraining). Total knee joints of rat were collected at week 0,1,2,3,and 6,fixed with 4% PFA,embedded in paraffin, sectioned and stained. Cartilage morphological changes were observed by Safranin-O staining, NO/i NOS content changes in serum and synovial fluid were determined by ELISA,Mankin's scores were obtained, and cartilage pathological changes were analyzed. Results Overtraining resulted in cell sorting disorders, loss of matrix, destruction like rough surface and cracks, and degeneration of cartilage pathology process. Levels of NO/i NOS in serum and synovial fluid increased after overtraining:week 1(48.088±1.534),(3.058±0.073);week 2(54.574±0.800),(6.630±0.524);week 3(68.020±3.184),(7.384±0.306);weeks 6(104.394±8.094),(11.364±0.882).Levels of NO/i NOS in synovial fluid group were: week 1(65.814 ±1.610),(8.126 ±0.724); week 2(72.766±2.344),(9.694±0.564);week 3(88.816±0.819),(11.326±1.473);week 6(120.204±12.044),(15.166±1.198),and high level of concentration was found with the increasing amount of exercise.Conclusion There is a positive correlation between the levels of NO/i NOS in synovial fluid or serum and the extent of the damage of articular cartilage.
- Nitric Oxide /
- Induced Nitricoxide Synthase /
- Cartilage /
- Osteoarthritis

HTML全文

参考文献(32)

[1]	[1]SALEH K J,DAVIS A.Measures for Pain and Function Assessments for Patients With Osteoarthritis[J].J Am Acad Orthop Surg,2016,24(11):e148-e162.
[2]	[2]SCANZELLO C R.Role of low-grade inflammation in osteoarthritis[J].Curr Opin Rheumatol(Epub ahead of print DOI:10.1097/BOR.0000000000000353).
[3]梁永生,李芳祥.备战第28届奥运会中国优秀运动员运动损伤调查分析[J].中国运动医学杂志,2007,11,26(6):744-745.
[4]	[4]FELSON D T.Obesity and vocational and avocational overload of the joint as risk factors for osteoarthritis[J].JRheumatol Suppl,2004,70(2):2-5.
[5]邓紫玉,孙鹥,林俊,等.ADAMTS-4在膝骨关节炎关节液中的表达和意义[J].昆明医学院学报,2011,32(6):59-61.
[6]张晓磬,张凤兰,万崇华,等.慢性病患者生命质量测定量表体系之骨关节炎量表的研制及考评[J].昆明医科大学学报,2013,34(8):23-27.
[7]	[7] TANG T,MUNETA T,SEKIYA I.Fibrous change of the infrapatellar fat pad due to strenuous running exercise and its treatment with intraarticular hyaluronan injection in rat mode[lJ].J Med Sci,2008,55(1):163-173.
[8]	[8]PANDOL S J,PERISKIC S,GUKOVSKY I,et al.Ethanol diet increases the sensitivity of rats to pancreatitis induced by cholecystokinin octapeptide[J].Gastroenterology,1999,117(3):706-716.
[9]	[9]NAH S S,CHOI I Y,LEE C K,et al.Effects of advanced glycation end products on the expression of COX-2,PGE2and NO in human osteoarthritic chondrocytes[J].Rheumatology(Oxford),2008,47(4):425-431.
[10]	[10] TANG T,MUNETA T,JU Y J,et al.Serum keratan sulfate transiently increases in early stage of osteoarthritis during strenuous running of rats:Protective effect of intraarticular hyaluronan injection[J].Arthritis Res Ther,2008,10(1)1-8.
[11]	[11]MANKIN H J,DORFMAN H,LIPPIELLO L,et al.Biochemical and metabolic abnormalities in articular cartilage from osteoarthritic human hips.Ⅱ.Correlation of morphology with biochemical and metabolic data[J].J Bone Joint Surg Am,1971,53(3):523-537.
[12]	[12]PAP G,EBERHARDT R,STRMER I,et al.Developent of osteoarthritis in the knee joints of Wistar rats strenuous running exercise in a running wheel by intracranial self-stimulation[J].Pathol Res Pract,1998,194(1):41-47.
[13]	[13]BURGHARDT P R,FULK L J,HAND G A,et al.The effects of chronic treadmill and wheel running on behavior in rats[J].Brain Res,2004,1019(1-2):84-96.
[14]	[14] SEKIYA I,TANG T,HAYASHI M,et al.Periodic knee injections of BMP-7 delay cartilage degeneration induced by excessive running in rats[J].J Orthop Res,2009,27(8):1088-1092.
[15]	[15]SASAKI K,HATTORI T,FUJISAWA T,et al.Nitric oxide mediates interleukin-1-induced gene expression of matrix metalloproteinases and basic fibroblast growth factor in cultured rabbit articular chondrocytes[J].J Biochem,1998,123(3):431-439.
[16]	[16]CLANCY R M,REDISKE J,TANG X,et al.Outside-in signaling in the chondrocyte.Nitric oxide disrupts fibronectin-induced assembly of a subplasmalemmal actin/rho A/focal adhesion kinase signaling complex[J].JClin Invest,1997,100(7):1789-1796.
[17]	[17]TAMURA T,NAKANISHI T,KIMURA Y,et al.Nitric oxide mediates interleukin-1-induced matrix degradation and basic fibroblast growth factor release in cultured rabbit articular chondrocytes:a possible mechanism of pathological neovascularization in arthritis[J].Endocrinology,1996,137(9):3729-3737.
[18]	[18]STADLER J,STEFANOVIC-RACIC M,BILLIAR TR,et al.Articular chondrocytes synthesize nitric oxide in response to cytokines and lipopolysaccharide[J].JImmunol,1991,Dec 1;147(11):3915-3920.
[19]	[19]SASSI N,GADGADI N,LAADHAR L,et al.Notch signaling is involved in human articular chondrocytes de-differentiation during osteoarthritis[J].J Recept Signal Transduct Res,2014,34(1):48-57.
[20]	[20]DAOUTI S,LATARIO B,NAGULAPALLI S,et al.Development of comprehensive functional genomic screens to identify novel mediators of osteoarthritis[J].Osteoarthritis Cartilage,2005,13(6):508-518.
[21]	[21]YIN S L.Effect of exercise on endothelin and nitric oxide synthesis of thoracic aorta in rat[J].Chinese Journal of Sports Medicine,1998,17(1):8-9.
[22]	[22]ZHOU Y,LIU S Q,YU L,et al.Berberine prevents nitric oxide-induced rat chondrocyte apoptosis and cartilage degeneration in a rat osteoarthritis model via AMPK and p38 MAPK signaling[J].Apoptosis,2015,20(9):1187-1199.
[23]	[23]BALAGANUR V,PATHAK NN,LINGARAJU M C,et al.Effect of S-methylisothiourea,an inducible nitric oxide synthase inhibitor,in joint pain and pathology in surgically induced model of osteoarthritis[J].Connect Tissue Res,2014,55(5-6):367-377.
[24]	[24]CUZZOCREA S.Role of nitric oxide and reactive oxygen species in arthritis[J].Curr Pharm Des,2006,12(27):3551-3570.
[25]	[25]KHOJAH H M,AHMED S,ABDEL-RAHMAN M S,et al.Reactive oxygen and nitrogen species in patients with rheumatoid arthritis as potential biomarkers for disease activity and the role of antioxidants[J].Free Radic Biol Med,2016,97:285-291.
[26]	[26]TURKON H,GOKMEN F,AKIR D U,Increased Levels of Serum Ischemia Modified Albumin in Patients with Ankylosing Spondylitis[J].Clin Lab,2016,62(4):645-649.
[27]	[27]WAHL S M,MCCARTNEY-FRANCIS N,CHAN J,et al.Nitric oxide in experimental joint inflammation.Benefit or detriment[J].Cells Tissues Organs,2003,174(1-2):26-33.
[28]	[28]ABRAMSON S B.Nitric oxide in inflammation and pain associated with osteoarthritis[J].Arthritis Res Ther,2008,10(Suppl 2):S2.
[29]	[29]ABRAMSON S B.Osteoarthritis and nitric oxide[J].Osteoarthritis Cartilage,2008,16(2):S15-20.
[30]	[30]TAKAHASHI T,KONDOH T,OHTANI M,et al.Association between arthroscopic diagnosis of temporomandibular joint osteoarthritis and synovial fluid nitric oxide levels[J].Oral Surg Oral Med Oral Pathol Oral Radiol Endod,1999,88(2):129-136.
[31]	[31]KOBAYASHI K,MATSUZAKA S,YOSHIDA Y,et al.The effects of intraarticularly injected sodium hyaluronate on levels of intact aggrecan and nitric oxide in the joint fluid of patients with knee osteoarthritis[J].Osteoarthritis Cartilage,2004,12(7):536-542.
[32]王伟,李彦林,贾福,等.骨关节炎患者软骨细胞的体外培养技术[J].昆明医学院学报,2008,29(1):149-152.

相关文章(20)

[1]	谢飞飞, 辛隐子, 徐敏, 李景涵, 王伟. 长链非编码RNA在软骨发育及骨关节炎中作用机制的研究进展, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20241001
[2]	陈一晗, 张善勇, 丁昱, 张莉. 颞下颌关节骨关节炎分子致病机制的研究进展, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20240701
[3]	邓绍友, 李蓉, 李进涛, 赵玉兰, 王佩锦, 郑红. 基于网络药理学探讨恒古骨伤愈合剂治疗骨关节炎的机制及动物实验初步验证, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230701
[4]	皇甫文丽, 黄瑶, 刘波, 吕长海, 刘娟, 代自超. 建立大鼠颞下颌关节骨关节炎动物模型的2种方法比较, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230308
[5]	吕煜, 胡义波, 王嘉鑫, 苏有橦, 代佑果. 蛋白质S-亚硝基化与消化系统肿瘤关系的研究进展, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220911
[6]	王子涵, 叶改映, 赵涛, 张俊, 胡瑜. mTOR在SD大鼠TMJOA髁突软骨中表达变化的实验研究, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20221033
[7]	陈先维, 陆向东, 王永峰, 赵轶波, 杨旭, 王文轩, 范彦鑫, 张晖, 赵斌. 骨关节炎患者单核细胞/淋巴细胞比值、红细胞压积与疾病活动度的相关性, 昆明医科大学学报.
[8]	闫慧玲, 赵宏斌, 钱传云, 王应忠, 张洋, 沈相霖, 蒋罡, 高爽. 膝骨关节炎疗效评估的研究进展, 昆明医科大学学报.
[9]	奉永泉, 王健龙, 刘阳, 饶磊. 关节镜清理结合腓骨近端截骨术对膝内翻骨关节炎的治疗效果, 昆明医科大学学报.
[10]	陈忠义, 乔廷廷, 董宝莲, 郭玲. LPS介导三种啮齿动物原代胶质细胞炎症氧化反应的研究, 昆明医科大学学报.
[11]	孙宇星, 谭力, 郝芮, 李明, 甘政艳, 吴澄清. 儿童肺炎支原体肺炎与呼出气一氧化氮的相关性, 昆明医科大学学报.
[12]	李雪武. 依托芬那酯凝胶与双氯芬酸钠治疗膝骨关节炎的临床比较, 昆明医科大学学报.
[13]	黄胜华, 李晓霞, 连希艳, 陈子摇, 赵劲涛, 冯志坚. 尿酸在氧化应激状态下对人脐静脉内皮细胞的影响, 昆明医科大学学报.
[14]	代龙金. β-连环蛋白与膝骨关节炎相关性研究, 昆明医科大学学报.
[15]	张晓磬. 慢性病患者生命质量测定量表体系之骨关节炎量表的研制及考评, 昆明医科大学学报.
[16]	张晓磬. 慢性病患者生命质量测定量表体系之骨关节炎量表的研制及考评, 昆明医科大学学报.
[17]	李福萍. 阿伐他汀对原发性高血压患者硫化氢和一氧化氮水平的影响, 昆明医科大学学报.
[18]	. ADAMTS-4在膝骨关节炎关节液中的表达和意义, 昆明医科大学学报.
[19]	宋宇锋. 去势大鼠关节软骨病理变化的研究, 昆明医科大学学报.
[20]	王伟. 骨关节炎患者软骨细胞的体外培养技术, 昆明医科大学学报.

施引文献

期刊类型引用(6)

1.	刘科，胡涵，侯占仙，龚红月，许寒. 2022年自贡市乳腺DR摄影受检者剂量调查分析. 中国辐射卫生. 2023(05): 522-526 . 百度学术
2.	王青宏，王晓成，蔡敏，王建明. 全视野数字乳腺摄影结合乳腺数字体层合成摄影的辐射剂量与压迫厚度及乳房密度的相关性研究. 中华放射医学与防护杂志. 2022(08): 645-650 . 百度学术
3.	李玲玲，钱银锋，王弢，李丽. 乳腺癌筛查中乳腺X射线摄影两种标准体位及乳腺压迫厚度与平均腺体剂量的关系. 辐射防护. 2022(05): 495-500 . 百度学术
4.	曲留更，王晓静. 数字乳腺三维断层摄影技术剂量与图像质量的相关性研究. 泰山医学院学报. 2020(12): 887-889 . 百度学术
5.	汪湍，帅建军，蔡斌，晋婉露，文智. 新疆地区女性乳腺腺体分型差别及其对乳腺癌诊断价值的分析. 中国医学前沿杂志(电子版). 2019(02): 51-54 . 百度学术
6.	李广民，林志凯，彭如臣，冯泽臣，邳建庭，王子文，姚剑，郭红玉，孙伟. 甲状腺-乳腺防护衣在乳腺X线摄影中降低辐射剂量的研究. 中国医学物理学杂志. 2019(06): 653-657 . 百度学术