多西紫杉醇联合伽马刀对肝癌细胞裸鼠皮下移植瘤凋亡作用的影响

李静娴; 何迷; 柳瑞; 邢艺苑; 简远熙; 王家平; 赵睿敏

多西紫杉醇联合伽马刀对肝癌细胞裸鼠皮下移植瘤凋亡作用的影响

1. 昆明医科大学研究生部
2. 昆明医科大学校医院放射科

基金项目:

基金：云南省社会发展科技计划应用基础研究基金资助项目(2009ZC111M);

计量
- 文章访问数: 2075
- HTML全文浏览量: 579
- PDF下载量: 54
- 被引次数: 0
出版历程
- 收稿日期: 2016-05-14

The Apoptosis Effect of Docetaxel Combined with Gamma Knife on Hepatoma Cells Xenograft in Nude Mice

Funds:

基金：云南省社会发展科技计划应用基础研究基金资助项目(2009ZC111M);

摘要

摘要: 目的探讨多西紫杉醇联合伽马刀对肝癌细胞株裸鼠皮下移植瘤细胞凋亡作用的影响.方法成功建立:SMMC-7721肝癌细胞株裸鼠皮下移植瘤模型,分2组,每组各10只裸鼠,即多西紫杉醇联合伽马刀组及肿瘤对照组.实验组先用注射多西紫杉醇处,剂量为60[μg/(0.3 m L·只)],每3天注射1次,连续注射6次,隔天伽马刀照射1次,连续伽马刀照射6次.(室温下用137Cs放射源照射局部肿瘤,照射按分割照射每次分别给予5 Gy总剂量为10 Gy).对照组注射生理盐水,剂量同前.观察2组的肿瘤生长情况.30 d后切取瘤样组织,用TUNEL法检测肿瘤细胞凋亡率.结果实验组的肿瘤生长速率明显减慢,实验组的凋亡指数(35.03±1.51)%明显高于对照组(0.87±0.39)%,两者间差异有统计学意义(P<0.05).结论多西紫杉醇联合伽马刀具有促进肿瘤细胞凋亡进而抑制肝细胞癌裸鼠移植瘤生长作用.
- 多西紫杉醇 /
- 伽马刀 /
- 裸鼠移植瘤 /
- 凋亡指数
Abstract: Objective To explore the apoptosis effect of docetaxel combined gamma knife on hepatoma cell SMMC-7721 subcutaneous xenograft in nude mice. Methods Subcutaneous xenograft models were constructed and were divided into two groups: control group and experimental group. The experimental group was treated with docetaxel 60ug/0.3ml once every 3 days for 6 times and gamma irradiation once every other day for 6 times(with indoor temperature of137 Cs radiation source irradiating the tumor and of fractionated schedule 5Gy with the total dose of 10 Gy every time). The control group was treated with physiological saline with the same dose of 60 ug/0.3 m L.Tumor growth was observed. Tumor samples were cut 30 days after the treatment and TUNEL was used to detect the apoptosis of tumor cells. Results Tumor growth rate in experimental group significantly slowed down. Apoptotic index in experimental groups was significantly higher than that in control group(P < 0.05). Conclusion Docetaxel combined gamma knife can inhibit the growth of hepatocellular carcinoma in nude mice.
- Docetaxel /
- Gamma knife /
- Xenograft in nude mice /
- Apoptotic index

HTML全文

参考文献(31)

[1]	[1]ESPOSTI D D,HERNANDEZ-VARGAS H,VOEGELE C,et al.Increased m RNA levels of sphingosine kinases and S1P lyase and reduced levels of S1P were observed in hepatocellular carcinoma in association with poorer differentiation and earlier recurrence[J].Oncotarget,2016,10(3):7364-7366.
[2]	[2]URANBILEG B,IKEDA H,KURANO M,et al.Increased m RNA levels of sphingosine kinases and S1P lyase and reduced levels of S1P were observed in hepatocellular carcinoma in association with poorer differentiation and earlier recurrence[J].PLo S One,2016,11(2):01459-01462.
[3]	[3]IZUMIH,KANEKOY.Evidence of Asymmetric cell division and centroso meinheritance in humanneuroblastoma cells[J].Proc Natl Acad Sci USA,2012,109(44):18048-18053.
[4]	[4]THUSS-PATIENCE P C,KRETZSCHMAR A,DOGAN Y,et al.Docetaxel and capecitabine for advance gastric cancer:investigating dose-dependeng efficacy in two patient cohorts[J].Br J Cancer,2011,105(4):505-512.
[5]	[5]LU G,ZHANG G,ZHENG X,et al.c9,t11-conjugated linoleic acid induces HCC cell apoptosis and correlation with PPAR-γsignaling pathway[J].Am J Transl Res,2015,7(12):2752-2763.
[6]	[6]KONTZOGLOUK,PALLAV,KARAOLANISG.Correlatiot between ki67 and breast cancer prognosis[J].Oncology,2013,84(4):219-225.
[7]	[7]ZHANG B,LIU K,ZHANG J,et al.Glutamine synthetase predicts adjuvant TACE response in hepatocellular carcinoma[J].Clin Exp Med,2015,8(11):20722-20731.
[8]	[8]CHEN J,BAI T.The safety and efficacy of laparoscopic and open hepatectomy in hepatocellular carcinoma patients with liver cirrhosis:a systematic review[J].Clin Exp Med,2015,8(11):20679-20689.
[9]	[9]ZHU S L,CHEN J.Efficacy of hepatic resection for huge(≥10 cm)hepatocellular carcinoma:good prognosis[J].Clin Exp Med,2015,8(11):20581-20588.
[10]	[10]TONG L,CHENW,WU J.Folic acid-coupled nanopaclitaxel lipome reverses dru resistance in SKOV3/TAX ovarian cancer cells[J].Anti-Cancer Dru,2014,25(3):244-254.
[11]	[11]ELDAR-BOOCK A,POLYAK D,SCOMPARIN A,et al.Nano-sized polymers and liposomes,desined to deliver combinat ion therapy for cancer[J].Curr Opin Biotech,2013,24(4):682-689.
[12]	[12]SAW P E,KIM S,LEE I,et al.Aptide-conjuated liposome targeting tumor associated fibronectin for lioma therapyd[J].J Mater Chem B,2013,1(37):4723-4726.
[13]	[13]LI L,TEN HAGENTLM,HOSSANN M,et al.Mild hyperthermia triggered doxorubicin release form optimized stealth thermosensitive liposomes improves intratumoral drug delivery and efficacy[J].J Control Release,2013,168(2):142-150.
[14]何迷,王家平.多西紫杉纯在肝癌细胞裸鼠移植瘤中对P53、VEGF作用的研究[J].昆明医科大学学报,2015,36(1):43-47.
[15]孙寅,王家平.原发性肝癌的临床介入治疗发展[J].昆明医科大学学报,2013,6(2):123-126.
[16]马友龙,祁海艳.多西紫杉醇纳米脂质体的制备及其对肝癌细胞的体外治疗研究[J].中国生化药物杂志,2015,35(6):43-47.
[17]巫蒙,王梅.肿瘤多耐药性产生机制及其逆转的研究现状[J].当代医学,2011,17(21):26-28.
[18]魏丽艳,崔纯莹,吴建辉,等.以海豹油为基质的多西紫杉醇脂肪乳的制备及其抗肿瘤活性研究[J].首都医科大学学报,2015,36(2):192-198.
[19]陈建国.中国肝癌发病趋势和一级预防[J].临床肝胆病杂志,2012,4(28):256-260.
[20]	[20]KASEB AO,XIAO L,NAGUIB R,et al.Abstract C26:Development and validation of a scoring system using insulin-like growth factor to assess hepatic reserve in hepatocellular carcinoma[J].Mol Cancer Ther,2013,12(11Supplement):C26-C28.
[21]	[21]ZHANG C Z,ZHANG H,YUN J,et al.Dihydroartemisinin exhibits antitumor activity toward hepatocellular carcinoma in vitro and in vivo[J].Biochem Pharmacol,2012,83(9):1278-1289.
[22]	[22]MINERO V G,DE STEFANIS D,COSTELLI P,et al.In vitro and in vivo conditional sensitization of hepatocellular carcinoma cells to TNFinduced apoptosis by Taxol[J].Cell Cycle,2015,14(7):1090-1102.
[23]康娟,吴小翔,张大志,等.载多西紫杉醇脂质微泡联合超声靶向微泡破裂对人肝癌细胞增殖和凋亡影响的初步研究[J].重庆医科大学学报,2014,12(39):1698-1702.
[24]	[24]MUTHU M S,KULKARNI S A,RAJU A,et al.Theranostic liposomes of TPGS coating for targeted co-delivery of docetaxel and quantum dots[J].Biomaterials,2012,33(12):3494-3501.
[25]	[25]DAKWAR G R,HAMMAD I A,POPOV M,et al.Delivery of proteins to the brain by bolaamphiphilic nano-sized vesicles[J].J Control Release,2012,160(2):315-321.
[26]	[26]SUN N,MENG Q,TIAN A,et al.Nanoliposome-mediated FL/TRAIL double-gene therapy for colon cancer:in vitro and in vivo evaluation[J].Cancer Lett,2012,315(1):69-77.
[27]	[27]BARENHOLZ Y.Doxil-the first FDA-approved nanodrug:lessons learned[J].J Control Release,2012,160(2):117-134.
[28]	[28] TONG L,CHEN W,WU J,et al.Folic acid-coupled nano-paclitaxel liposome reverses drug resistance in SKOV3/TAX ovarian cancer cells[J].Anti-Cancer Drug,2014,25(3):244-254.
[29]	[29]DUA J S,RANA A C,BHANDARI A K.Liposome:methods of preparation and applications[J].Int J Pharm Stud Res,2012,(3):14-20.
[30]黄红兵,刘韬,林子超,等,多西紫杉醇脂质体的制备及其在家兔体内的药代动力学[J].癌症,2007,26(12):1287-1291.
[31]	[31] ELDAR-BOOCK A,POLYAK D,SCOMPARIN A,et al.Nano-sized polymers and liposomes designed to deliver combination therapy for cancer[J].Curr Opin Biotech,2013,24(4):682-689.

相关文章(20)

[1]	张雪莎, 周杰, 李彩琳, 吴定宇, 彭芳. 美洲大蠊提取物对肝癌皮下移植瘤裸鼠自噬和侵袭转移的影响, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20231018
[2]	王硕, 茹琪, 张晓婵, 杨志瑜, 王乐, 李柳. MTHFR C677T联合CYP2C19基因检测在紫杉醇释放冠脉球囊导管术后应用, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220618
[3]	王硕, 孙爱, 张慧杰, 尤杨, 魏立业, 李柳. 心肌缺血预适应在紫杉醇释放冠脉球囊导管扩张术中的应用, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20211225
[4]	张智显, 顾后, 林劼, 雷学芬, 江利锋, 李楠, 路欣妍, 李亭葶. 伽玛刀联合阿帕替尼及替莫唑胺治疗肺癌脑转移的疗效, 昆明医科大学学报.
[5]	张智显, 顾后, 林劼, 曹洪明, 雷学芬, 江利锋. 伽马刀联合贝伐单抗对比替莫唑胺治疗术后恶性脑胶质瘤的临床疗效, 昆明医科大学学报.
[6]	侯峰强, 董山潮, 雷喜锋, 张伟. 紫杉醇对人胆管癌QBC939细胞wnt/β-catenin信号通路基因和蛋白表达的影响, 昆明医科大学学报.
[7]	宋飞, 向盈盈, 毛志坚, 张小文. 2种不同方法制备的紫杉醇壳聚糖缓释膜的药物缓释特性对比, 昆明医科大学学报.
[8]	唐诗聪, 潘泓, 黄耀元, 周鑫, 王守峰, 左传田, 茅乃权. 人非小细胞肺癌裸鼠移植瘤模型的建立, 昆明医科大学学报.
[9]	陈鑫, 黄璞, 贾楠楠, 陈绍春, 叶频, 刘承杏, 李国萍. 鼻咽癌细胞株CNE-2及其亚克隆S-18裸鼠皮下移植生长速度比较, 昆明医科大学学报.
[10]	陈楠. 培美曲塞联合顺铂与紫杉醇联合顺铂治疗晚期非小细胞肺癌的临床疗效观察, 昆明医科大学学报.
[11]	王琨. shRNA-Bmi1重组载体构建及其对胆囊癌裸鼠移植瘤hTERT表达的影响, 昆明医科大学学报.
[12]	熊伟. 奥沙利铂化疗对荧光HepG2肝癌细胞裸鼠原位移植瘤侵袭转移潜能的影响, 昆明医科大学学报.
[13]	吴京蔓. 舌鳞癌Cal27裸鼠移植瘤模型的建立及其意义, 昆明医科大学学报.
[14]	何迷. 多西紫杉醇在肝癌细胞裸鼠肝脏移植瘤中对P53、VEGF作用的研究, 昆明医科大学学报.
[15]	陈学平. 氨培养胎鼠神经细胞钙离子浓度变化与凋亡的相关性, 昆明医科大学学报.
[16]	唐诗聪. 组织块移植法建立人肺癌裸鼠移植瘤模型, 昆明医科大学学报.
[17]	韩江琼. 多西紫杉醇与紫杉醇联合吡柔比星和环磷酰胺在局部进展期乳腺癌化疗中致心脏毒性的观察, 昆明医科大学学报.
[18]	吴静. 前列腺癌细胞株PC-3经Doc处理后p-c-jun和AR的表达变化, 昆明医科大学学报.
[19]	. 人脑胶质瘤中proNGF凋亡前信号的研究, 昆明医科大学学报.
[20]	卢永刚. 茉莉酸甲酯对人肝癌细胞HepG-2裸鼠皮下移植瘤生长抑制作用的研究, 昆明医科大学学报.