多西紫杉醇联合伽马刀对肝癌细胞裸鼠皮下移植瘤凋亡作用的影响

李静娴; 何迷; 柳瑞; 邢艺苑; 简远熙; 王家平; 赵睿敏

多西紫杉醇联合伽马刀对肝癌细胞裸鼠皮下移植瘤凋亡作用的影响

1. 昆明医科大学研究生部
2. 昆明医科大学校医院放射科

基金项目:

基金：云南省社会发展科技计划应用基础研究基金资助项目(2009ZC111M);

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出版历程
- 收稿日期: 2016-05-14

The Apoptosis Effect of Docetaxel Combined with Gamma Knife on Hepatoma Cells Xenograft in Nude Mice

Funds:

基金：云南省社会发展科技计划应用基础研究基金资助项目(2009ZC111M);

摘要

摘要: 目的探讨多西紫杉醇联合伽马刀对肝癌细胞株裸鼠皮下移植瘤细胞凋亡作用的影响.方法成功建立:SMMC-7721肝癌细胞株裸鼠皮下移植瘤模型,分2组,每组各10只裸鼠,即多西紫杉醇联合伽马刀组及肿瘤对照组.实验组先用注射多西紫杉醇处,剂量为60[μg/(0.3 m L·只)],每3天注射1次,连续注射6次,隔天伽马刀照射1次,连续伽马刀照射6次.(室温下用137Cs放射源照射局部肿瘤,照射按分割照射每次分别给予5 Gy总剂量为10 Gy).对照组注射生理盐水,剂量同前.观察2组的肿瘤生长情况.30 d后切取瘤样组织,用TUNEL法检测肿瘤细胞凋亡率.结果实验组的肿瘤生长速率明显减慢,实验组的凋亡指数(35.03±1.51)%明显高于对照组(0.87±0.39)%,两者间差异有统计学意义(P<0.05).结论多西紫杉醇联合伽马刀具有促进肿瘤细胞凋亡进而抑制肝细胞癌裸鼠移植瘤生长作用.
- 多西紫杉醇 /
- 伽马刀 /
- 裸鼠移植瘤 /
- 凋亡指数
Abstract: Objective To explore the apoptosis effect of docetaxel combined gamma knife on hepatoma cell SMMC-7721 subcutaneous xenograft in nude mice. Methods Subcutaneous xenograft models were constructed and were divided into two groups: control group and experimental group. The experimental group was treated with docetaxel 60ug/0.3ml once every 3 days for 6 times and gamma irradiation once every other day for 6 times(with indoor temperature of137 Cs radiation source irradiating the tumor and of fractionated schedule 5Gy with the total dose of 10 Gy every time). The control group was treated with physiological saline with the same dose of 60 ug/0.3 m L.Tumor growth was observed. Tumor samples were cut 30 days after the treatment and TUNEL was used to detect the apoptosis of tumor cells. Results Tumor growth rate in experimental group significantly slowed down. Apoptotic index in experimental groups was significantly higher than that in control group(P < 0.05). Conclusion Docetaxel combined gamma knife can inhibit the growth of hepatocellular carcinoma in nude mice.
- Docetaxel /
- Gamma knife /
- Xenograft in nude mice /
- Apoptotic index

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参考文献(31)

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