高脂饮食诱导妊娠期糖尿病大鼠模型的建立

张馨文; 张燕; 胡晏馨; 潘宝龙; 王名芳; 马润玫

高脂饮食诱导妊娠期糖尿病大鼠模型的建立

昆明医科大学第一附属医院妇产科

基金项目:

基金：国家自然科学基金资助项目(81160082); 云南省卫生厅内设研究机构资助项目(2014WS180); 昆明医科大学研究生创新基金资助项目(2015S13);

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出版历程
- 收稿日期: 2016-06-12

Establishment of Gestational Diabetes Rat Model Induced by High-fat Diet

Funds:

基金：国家自然科学基金资助项目(81160082); 云南省卫生厅内设研究机构资助项目(2014WS180); 昆明医科大学研究生创新基金资助项目(2015S13);

摘要

摘要: 目的通过高脂饮食喂养的方法建立妊娠期糖尿病大鼠模型.方法选取雌性SD大鼠55只,随机分为正常饮食未孕组、正常饮食怀孕组、高脂饮食未孕组、高脂饮食怀孕组,喂养3月后孕鼠组与雄性大鼠2:1合笼,各组继续给予高脂或普通饲料,分别于孕前及妊娠1、7、14、20 d测空腹血糖与体重,用酶联免疫吸附法动态监测空腹胰岛素和血清C肽值并计算胰岛素抵抗指数,于妊娠末期评估大鼠的葡萄糖耐量和血脂代谢指标,取大鼠肝脏和胰腺组织切片染色并做镜下观察.结果高脂大鼠体重明显高于对照鼠,孕期体重显著增加(P<0.05),在孕期高脂孕鼠的空腹血糖,胰岛素,C肽水平都显著高于正常怀孕大鼠,胰岛素抵抗明显(P<0.05),孕晚期高脂怀孕大鼠的葡萄糖耐量曲线下面积、血脂水平都明显升高(P<0.05).结论使用高脂饮食诱导可成功建立妊娠期糖尿病(gestational diabetes mellitus,GDM)的大鼠模型,此模型接近人类妊娠糖尿病的发病特点,为今后的广泛研究提供了媒介.
- 高脂饮食 /
- 妊娠糖尿病 /
- 大鼠 /
- C肽
Abstract: Objective To establish a gestational diabetes rat model by feeding the rats with high-fat diet.Methods A total of 55 female SD rats were divided into four groups: NV group, NP group, FV group and FP group. Three months after normal feeding, the female rats in NP and FP group were put into the same cage with the male rats at the ratio of 2:1 and were given high-fat diet or normal diet as usual. Before pregnancy and day1,7,14,20 in pregnancy,fasting plasma glucose and body weight of rats were detected. The fasting serum insulin and serum c-peptide levels were monitored by enzyme immunoassay and insulin resistance index was calculated. At late pregnancy, glucose tolerance and the indicator of fat were tested. Liver and pancreas were dyed to be observed under microscope. Results Body weights of the rats raised with high-fat diet were significantly higher than those of control group and body weight during pregnancy significantly increased(P<0.05). Fasting glucose,fasting insulin and serum C-peptide in FP group were significantly higher than those in NP group and insulin resistance was evident(P<0.05). The area under curve of GTT in FP group was significantly larger(P<0.05).The levels of serum lipids in FP group were higher than those in normal group. Conclusions The gestational diabetes rat model induced by high-fat diet can be successfully established.The model presents major pathophysiological manifestations of GDM and can be used as a good model of GDM in relevant research.
- High-fat diet /
- Gestational diabetes mellitus /
- Rat /
- C peptide

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参考文献(20)

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