黄藤素注射液抗脂多糖致小鼠急性肺损伤的作用

王应霞; 杨桂梅; 胡艳文; 王永艳; 姚珊; 张旋; 张丽

黄藤素注射液抗脂多糖致小鼠急性肺损伤的作用

1. 昆明医科大学第一附属医院病理科
2. 昆明医科大学药学院暨云南省天然药物药理重点实验室
3. 昆明医科大学学报编辑部

基金项目:

基金：国家自然科学基金资助项目(81460565); 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目(2013FB111); 云南省中青年学术和技术带头人后备人才基金资助项目(2015HB042);

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出版历程
- 收稿日期: 2016-10-10

Protective Effect of Fibrauretin Injection Against Acute Lung Injury Induced by Lipopolysaccharide in Mice

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摘要

摘要: 目的研究黄藤素注射液抗脂多糖致小鼠急性肺损伤的作用.方法健康雄性成年昆明小鼠72只,随机分为6组:正常对照组;LPS组(气管滴入脂多糖LPS 5 mg/kg);氢化可的松组(腹腔注射氢化可的松3.3mg/kg,每日1次,连续3 d,第4天气管滴入LPS 5 mg/kg);黄藤素低、中、高剂量组(分别腹腔注射黄藤素2mg/kg、10 mg/kg和50 mg/kg,每日1次,连续3 d,第4天气管滴入LPS 5 mg/kg).在气管滴入LPS后24 h处死各组小鼠,部分小鼠取出肺组织进行称重计算肺系数,部分小鼠行支气管肺泡灌洗,对炎症细胞进行计数.结果LPS组小鼠肺系数明显高于正常对照组(P<0.05);氢化可的松组和黄藤素低、中、高剂量组小鼠肺系数明显低于LPS组(P<0.05).对于BALF中炎症细胞及中性粒细胞比例,LPS组明显高于正常对照组(P<0.01),与LPS组相比,氢化可的松组和黄藤素中剂量组和高剂量组明显降低(P<0.01).结论黄藤素注射液能够明显减轻脂多糖致小鼠急性肺损伤炎症反应程度.
- 黄藤素 /
- 急性肺损伤 /
- 支气管肺泡灌洗液 /
- 炎症细胞 /
- 肺系数
Abstract: Objective To investigate the protective effect of fibrauretin injection against acute lung injury induced by lipopolysaccharide(LPS) in mice.Methods Seventy-two healthy male adult Kunming mice were randomly divided into 6 goups:normal control group,LPS group(intratracheal instilation of 5 mg/kg of LPS),hydrocortisone group(intraperitoneal injection of 3.3 mg/kg of hydrocortisone,once daily for 3 days,and intratracheal instilation of 5 mg/kg of LPS on the 4th day) and low dose,medium dose and high dose of fibrauretin groups(intraperitoneal injection of 2,10 and 50 mg/kg of fibrauretin,respectively,once daily for 3 days,and intratracheal instilation of 5 mg/kg of LPS on the 4th day).The mice in each group were sacrificed by dislocation 24 h after intratracheal instilation of LPS.The lung tissues of partial mice in each group were extracted and weighed to calculate the lung coefficients,and bronchoalveolar lavage(BAL) was performed in partial mice in each group to collectthe BALF for counting the inflammatory cells.Results The lung cofficients of mice in LPS group were significantly higher than those in the normal control group(P<0.05).The lung cofficients of mice in hydrocortisone group, low dose, medium dose and high dose of fibrauretin groups were significantly lower than those in LPS group(P <0.05).For the percentage of inflammatory cells in BALF and the percentage of neutrophils in inflammatory cells,LPS group was significantly higher than the normal control group(P<0.01),and compared with LPS group,hydrocortisone group,low dose,medium dose and high dose of fibrauretin groups were significantly decreased(P<0.01).Conclusion Fibrauretin injection can significantly ameliorate the inflammatory reaction degree in mice with acute lung injury induced by LPS.
- Fibrauretin /
- Acute lung injury /
- BALF /
- Inflammatory cells /
- Lung coefficient

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参考文献(11)

[1]	[1]DUSHIANTHAN A,GROCOTT M P,POSTLE AD,et al.Acute respiratory distress syndrome and acute lung injury[J].Postgrad Med J,2011,87(1031):612-622.
[2]	[2]MATTHAY M A,ZEMANS R L.The acute respiratory distress syndrome:Pathogenesis and treatment[J].Annu Rev Pathol,2011,6(1):147-163.
[3]	[3]CARNESECCHI S,PACHE J C,BARAZZONE-ARGIROFFO C.NOX enzymes:Potential target for the treatment of acute lung injury[J].Cell Mol Life Sci,2012,69(14):2373-2385.
[4]	[4]SWEENEY R M,GRIFFITHS M,MCAULEY D.Treatment of acute lung injury:current and emerging pharmacological therapies[J].Semin Respir Crit Care Med,2013,34(4):487-498.
[5]国家药典委员会.中华人民共和国药典[M].北京:化学工业出版社,2010:391-392.
[6]张旋,薛凤麟,李莉,等.肽转运载体2 m RNA在脂多糖致急性肺损伤大鼠肺组织中的表达(英文)[J].中国药理学与毒理学杂志,2008,22(4):250-256.
[7]	[7]FU J,WANG Y,ZHANG J,et al.Anti-inflammatory and anti-apoptotic effects of oxysophoridine on lipopolysaccharide-induced acute lung injury in mice[J].Am J Transl Res,2015,7(12):2672-2682.
[8]	[8]ZHOU Z,YOU Z.Mesenchymal stem cells alleviate LPS-induced acute lung injury in mice by mir-142a-5p-c ontrolled pulmonary endothelial cell autophagy[J].Cell Physiol Biochem,2016,38(1):258-266.
[9]	[9]CHEN H,BAI C,WANG X.The value of the lipopolysaccharide-induced acute lung injury model in respiratory medicine[J].Expert Rev Respir Med,2010,4(6):773-783.
[10]王曾庚,聂祥碧,郭经华,等.参附对急性肺损伤大鼠热休克蛋白70表达的影响[J].南昌大学学报(医学版),2011,51(10):22-24.
[11]赵燕,何婧,管弦,等.黄芪注射液对急性肺损伤小鼠钠通道表达的影响[J].南方医科大学学报,2012,32(11):1603-1605,1609.

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