鼻咽癌细胞株CNE-2及其亚克隆S-18裸鼠皮下移植生长速度比较

陈鑫; 黄璞; 贾楠楠; 陈绍春; 叶频; 刘承杏; 李国萍

鼻咽癌细胞株CNE-2及其亚克隆S-18裸鼠皮下移植生长速度比较

1. 凤庆县人民医院外科
2. 昆明医科大学人体解剖学与组织胚胎学系
3. 昆明医科大学国际教育学院
4. 昆明医科大学第三附属医院头颈外科

基金项目:

基金：国家自然科学基金资助项目(81260195;81302362);

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出版历程
- 收稿日期: 2016-09-22

Growth Difference between CNE-2 and S-18 Cell Lines after Subcutaneous Xenograft

Funds:

基金：国家自然科学基金资助项目(81260195;81302362);

摘要

摘要: 目的研究鼻咽癌细胞株CNE-2及其亚克隆S-18在体内的生长速度差异及其可能机制.方法体外培养CNE-2和S-18细胞,以6×105细胞/只移植到裸鼠背部皮下,移植后3、7、10、14 d观测肿瘤大小;移植后第14天处死小鼠,称取肿瘤重量并检测肿瘤组织内的HSP27和NF-ΚB转录水平.结果 (1)裸鼠皮下移植后7 d,S-18已明显成瘤,CNE-2尚未成瘤;移植后10 d和14 d,2种细胞均明显成瘤,但S-18肿瘤体积[(223.13±21.32)mm3,10 d;(420.25±24.52)mm3,14 d]明显大于CNE-2肿瘤体积[(113.70±11.70)mm3,10 d;(279.86±25.78)mm3,14 d],P<0.05;移植后14 d S-18所形成的肿瘤的重量(0.521±0.137)g明显高于CNE-2所形成的肿瘤重量(0.449±0.125)g,P<0.05;(2)S-18肿瘤内的HSP27、NF-ΚB的转录水平明显高于CNE-2肿瘤,P<0.05.结论鼻咽癌细胞株S-18在体内的生长速度明显快于CNE-2细胞,HSP27和NF-ΚB可能参与了鼻咽癌细胞生长速度的调节.
- CNE-2细胞株 /
- S-18细胞株 /
- HSP27 /
- NF-κB /
- 肿瘤生长
Abstract: Objective To study the growth difference and possible mechanism between nasopharyngeal carcinoma(NPC) cell line CNE-2 and its subclone S-18.Methods CNE-2 and S-18 cells were cultured in vitro.6×105 cells/mouse were xenografted subcutaneously in the back of nude mice.The volumes of tumors were measured on the 3 rd,7 th,10 th,14 th day after grafting.Mice were sacrificed on the 14 th day and tumors were isolated and weighed.RNA from tumor tissues were extracted and transcriptional levels of HSP27 and NF-ΚB were detected.Results(1) S-18, instead of CNE-2, grew to form tumor mass 7 days after xenografting subcutaneously; both cell lines formed tumor mass 10 days after xenografting, however, the volumes of S-18 tumors [(223.13±21.32) mm3, 10 th day;(420.25±24.52) mm3,14 th day] were significant bigger than CNE-2 tumors [(113.70±11.70) mm3,10 th day;(279.86±25.78) mm3,14 th day];The weights of S-18 tumors were significantly higher than CNE-2 tumors on the 14 th day after xenografting;(2) The transcriptional levels of HSP27 and NF-ΚB in S-18 tumor were significantly higher than in CNE-2 tumor.Conclusion Xenografted S-18 NPC grows faster than Xenografted CNE-2 NPC.HSP27 and NF-κB are probably involved in the regulation of growth in NPC.
- CNE-2 cell line /
- S-18 cell line /
- HSP27 /
- NF-κB /
- Tumor growth

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参考文献(15)

[1]	[1]WANG S,MO Y,MIDORIKAWA K,et al.The potent tumor suppressor mi R-497 inhibits cancer phenotypes in nasopharyngeal carcinoma by targeting ANLN and HSPA4L[J].Oncotarget,2015,6(34):35893-35907.
[2]	[2] GU S Y,TANG W P,ZENG Y,et al.An epithelial cell line established from poorly differentiated nasopharyngeal carcinoma[J].Aizheng,1983,2(3):70-72.
[3]	[3]QIAN C N,BERGHUIS B,TSARFATY G,et al.Preparing the“soil”:the primary tumor induces vasculature reorganization in the sentinel lymph node before the arrival of metastatic cancer cells[J].Cancer Res,2006,66(11):10365-10376.
[4]	[4]LI G P,WANG H,LAI YK,et al.Proteomic profiling between CNE-2 and its strongly metastatic subclone S-18and functional characterization of HSP27 in metastasis of nasopharyngeal carcinoma[J].Proteomics,2011,11(14):2911-2920.
[5]	[5]ZONG D,YIN L,ZHONG Q,et al.ZNF488 enhances the invasion and tumorigenesis in nasopharyngeal carcinoma via the wnt signaling pathway involving epithelial mesenchymal transition[J].Cancer Res Treat,2016,48(1):334-344.
[6]	[6]NAITO S,VON ESCHENBACH A C,GIAVAZZI R,et al.Growth and metastasis of tumor cells isolated from a human renal cell carcinoma implanted into different organs of nude mice[J].Cancer Res,1986,46(8):4109-4115.
[7]苏胜发,卢泰祥.鼻咽癌治疗进展[J].肿瘤预防与治疗,2010,23(1):66-70.
[8]	[8]GU S Y,TANG W P,ZENG Y,et al.An epithelial cell line established from poorly differentiated nasopharyngeal carcinoma[J].Aizheng,1983,2(2):70-72.
[9]	[9]WU M S,WANG G F,ZHAO Z Q,et al.Smac mimetics in combination with TRAIL selectively target cancer stem cells in nasopharyngeal carcinoma[J].Mol Cancer Ther,2013,12(9):1728-1737.
[10]陈惠,渠景连,龚婕宁.现代医学对恶性肿瘤转移相关机制的研究进展[J].2014,39(15):2823-2827.
[11]	[11]LUO Y,LAN L,JIANG Y G,et al.Epithelial-mesenchy mal transition and migration of prostate cancer stem cells is driven by cancer-associated fibroblasts in an HIF-1α/β-catenin-dependent pathway[J].Mol Cells,2013,36(2):138-144.
[12]李国萍,陈绍春,李晓江,等.Hsp27基因对鼻咽癌细胞增殖的作用及其机制[J].肿瘤防治研究,2012,39(4):371-375.
[13]	[13]PAUL C,MANERO F,GONIN S,et al.Hsp27 as a negative regulator of cytochrome C release[J].Mol Cell Biol,2002,22(3):816-834.
[14]贾楠楠,邹智荣,李国萍,等.Gnaq对SH-SY5Y细胞增殖的作用及机制[J].昆明医科大学学报,2016,37(1):8-11.
[15]任改艳,孙阿宁,张晶晶,等.NF-κB在细胞凋亡中调节作用的研究进展[J].中国药理学与毒理学杂志,2015,29(2):323-326.

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