鼻咽癌细胞株CNE-2及其亚克隆S-18裸鼠皮下移植生长速度比较

陈鑫; 黄璞; 贾楠楠; 陈绍春; 叶频; 刘承杏; 李国萍

鼻咽癌细胞株CNE-2及其亚克隆S-18裸鼠皮下移植生长速度比较

1. 凤庆县人民医院外科
2. 昆明医科大学人体解剖学与组织胚胎学系
3. 昆明医科大学国际教育学院
4. 昆明医科大学第三附属医院头颈外科

基金项目:

基金：国家自然科学基金资助项目(81260195;81302362);

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出版历程
- 收稿日期: 2016-09-22

Growth Difference between CNE-2 and S-18 Cell Lines after Subcutaneous Xenograft

Funds:

基金：国家自然科学基金资助项目(81260195;81302362);

摘要

摘要: 目的研究鼻咽癌细胞株CNE-2及其亚克隆S-18在体内的生长速度差异及其可能机制.方法体外培养CNE-2和S-18细胞,以6×105细胞/只移植到裸鼠背部皮下,移植后3、7、10、14 d观测肿瘤大小;移植后第14天处死小鼠,称取肿瘤重量并检测肿瘤组织内的HSP27和NF-ΚB转录水平.结果 (1)裸鼠皮下移植后7 d,S-18已明显成瘤,CNE-2尚未成瘤;移植后10 d和14 d,2种细胞均明显成瘤,但S-18肿瘤体积[(223.13±21.32)mm3,10 d;(420.25±24.52)mm3,14 d]明显大于CNE-2肿瘤体积[(113.70±11.70)mm3,10 d;(279.86±25.78)mm3,14 d],P<0.05;移植后14 d S-18所形成的肿瘤的重量(0.521±0.137)g明显高于CNE-2所形成的肿瘤重量(0.449±0.125)g,P<0.05;(2)S-18肿瘤内的HSP27、NF-ΚB的转录水平明显高于CNE-2肿瘤,P<0.05.结论鼻咽癌细胞株S-18在体内的生长速度明显快于CNE-2细胞,HSP27和NF-ΚB可能参与了鼻咽癌细胞生长速度的调节.
- CNE-2细胞株 /
- S-18细胞株 /
- HSP27 /
- NF-κB /
- 肿瘤生长
Abstract: Objective To study the growth difference and possible mechanism between nasopharyngeal carcinoma(NPC) cell line CNE-2 and its subclone S-18.Methods CNE-2 and S-18 cells were cultured in vitro.6×105 cells/mouse were xenografted subcutaneously in the back of nude mice.The volumes of tumors were measured on the 3 rd,7 th,10 th,14 th day after grafting.Mice were sacrificed on the 14 th day and tumors were isolated and weighed.RNA from tumor tissues were extracted and transcriptional levels of HSP27 and NF-ΚB were detected.Results(1) S-18, instead of CNE-2, grew to form tumor mass 7 days after xenografting subcutaneously; both cell lines formed tumor mass 10 days after xenografting, however, the volumes of S-18 tumors [(223.13±21.32) mm3, 10 th day;(420.25±24.52) mm3,14 th day] were significant bigger than CNE-2 tumors [(113.70±11.70) mm3,10 th day;(279.86±25.78) mm3,14 th day];The weights of S-18 tumors were significantly higher than CNE-2 tumors on the 14 th day after xenografting;(2) The transcriptional levels of HSP27 and NF-ΚB in S-18 tumor were significantly higher than in CNE-2 tumor.Conclusion Xenografted S-18 NPC grows faster than Xenografted CNE-2 NPC.HSP27 and NF-κB are probably involved in the regulation of growth in NPC.
- CNE-2 cell line /
- S-18 cell line /
- HSP27 /
- NF-κB /
- Tumor growth

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参考文献(15)

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