手术双侧卵巢去势法建立骨质疏松树鼩模型

杨凤

手术双侧卵巢去势法建立骨质疏松树鼩模型

杨凤

(1)昆明学院,云南昆明 650214; 2)昆明医科大学第一附属医院急诊医学科,云南昆明 650032; 3)贵州医科大学附属医院骨外科,贵州贵阳 550004; 4)中国医学科学院中国协和医科大学医学生物学研究所,云南昆明 650118)

基金项目: ［基金项目］国家科技支撑计划子课题基金资助项目（2014BAI01B01-07）

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出版历程

Establishment of Tree Shrew Osteoporosis Model by Bilateral Ovariectomy

Yang Feng

(1)Kunming University,Kunming Yunnan 650214;2)Dept. of Emergency Medicine,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032;3)Dept. of Orthopedic Surgery,The Affiliated Hospital of Guizhou Medical University,Guiyang Guizhou 550004;4)Institute of Medical Biology, Chinese Academy of Medical Sciences and Peking Union Medical College,Kunming Yunnan 650118,China)

摘要

摘要: ［摘要］目的　采用手术双侧卵巢去势法建立骨质疏松树鼩模型．方法　60只健康 6 月龄雌性树鼩随机分成手术组（OVX，n＝30）与假手术组（SHAM，n＝30）．OVX 组切除双侧卵巢，SHAM 组切除与卵巢等体积的大网膜．2组分别于术前1 d及术后 1个月、2 个月、3 个月、4 个月、5个月、6 个月各随机抽取6只进行全身骨密度（BMD）检测；术前及术后 OVX 组与 SHAM 组比较 BMD 值有差异（P＜0.05），各组分别取6只树鼩安乐死，取其第2腰椎制成骨切片，行骨组织形态学观察．结果（1）实验过程中未出现死亡动物；（2）SHAM 组动物各时相点全身 BMD 值无差异（P≥0.05）；第2腰椎骨小梁完整，排列紧密、规则，小梁之间连接多见；骨髓细胞丰富，脂肪空泡少；（3）去势手术后 3 个月时，OVX 组全身 BMD 值明显低于 SHAM 组（P＜0.05）；骨小梁断裂、稀疏、变细，小梁间距增宽，排列不规则，小梁之间连接变少；骨髓细胞减少，出现较多的脂肪空泡；（4）术后 4 个月时，OVX 组全身 BMD 值显著低于 SHAM 组（P＜0.01）；骨小梁较 3 个月时更为稀疏，数量减少，小梁连接罕见，骨髓细胞进一步减少，脂肪空泡体积变大；（5）术后 6 个月时 OVX 组全身 BMD 值继续下降，下降幅度达到造模前的31%，与同时相点 SHAM 组比较差异具有统计学意义（P＜0.01）；骨小梁显著减少，骨髓细胞少见，脂肪空泡密集．结论　 6月龄雌性树鼩行手术双侧卵巢去势法造模 4 M 可建立稳定的骨质疏松树鼩模型．
- [关键词]树鼩 /
- 卵巢切除术 /
- 骨质疏松 /
- 骨密度 /
- 石蜡病理检查
Abstract: ［Abstract］Objective To establish the tree shrew osteoporotic model by bilateral ovariectomy．Methods　Sixty healthy female tree shrews（Tupaia belangeri chinensis）were divided into operation group（OVX，n＝30） and sham group （SHAM，n＝30）. The bilateral ovaries of the tree shrews in OVX group were operated to resect， while in SHAM omentum majus were removed with the same volume of the ovaries．The BMD on six animals randomly selected in two groups were detected on the day before operation and on 1st，2nd， 3rd，4th，5th and 6th month after operation respectively. Six tree shrews of each group were sacrificed respectively pre-operation and post-operation when there was a difference（P＜0.05）or a significant difference（P＜0.01）in BMD values between SHAM group and OVX group. Then the second lumbar vertebras were sectioned for histomorphometry. Results（1）No death occurred during experiment. （2）The BMD values of tree shrew's whole body in SHAM group at each time point had no difference（P≥0.05）；The trabecula in the second lumbar vertebras was complete， regular and closely arranged. The connection between bone trabecula was normal. Bone marrow cells were rich while fat vacuoles were few. （3）Three months after operation，the BMD value in the OVX group was obviously lower than that in the SHAM's（P<0.05）. The sections of the second lumbar vertebras in OVX group showed that the trabecular bone was broken，thinning and scattered，moreover the trabecular spacing was widened， irregular arranged and the connection between the trabecular meshwork became less while bone marrow cells were reduced and more fat vacuoles appeared. （4）After four months，the whole body BMD in OVX group was significantly lower than that in SHAM'S （P＜0.01）. The sections in OVX group showed the trabecular bone was sparser and thinner than that in the 3rd month， and the trabecular connection was rare while bone marrow cells were further reduced and fat vacuoles became larger.（5）Six months after operation， the whole body BMD value in OVX group declined by 31% with a significant BMD change（P＜0.01）compared with the value in pre-operation. The sections showed thinner trabecula， wider trabecular spacing，less bone marrow cells and more fat vacuoles. Conclusion　Bilateral ovariectomy on 6 month-old tree shrews could establish stable osteoporosis model after four months.
- ［Key words］Tree shrew；Ovariectomy；Osteoporosis；Bone mineral density；Paraffinsection examination /

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