Abstract: ［Abstract］Objective To investigate the effect of Slug and Sox9 on the invasion and metastasis of nasopharyngeal carcinoma stem cells. Methods　CD133 positive nasopharyngeal carcinoma stem cells were sorted from human nasopharyngeal carcinoma cell line CNE-2．Scratch test was used to detect cell invasion， migration and other biological behaviors when Slug and Sox9 together silenced by siRNA．Results　The invasion and migration abilities were significantly higher in CNE - 2 / CD133 + group compared with interference groups and control group at 24 hour 48 hour interference groups had little difference compare with control group，while lower at 48 hour. The metastases ability of CNE - 2/CD133 + group and control group was higher compared with interference groups'. Each interference group had no distinctive difference at each time. Conclusions　Silencing of slug and Sox9 has influence on nasopharyngeal cancer progression and metastasis. The relation among low expression tumor recurrence and patient survival time still need further research.