流式细胞检测肺结核患者肺泡灌洗液T淋巴细胞亚群评价
The Clinical Value of T Lymphocyte Subsets Analysis with Flow Cytometry in Bronchoalveolar Lavage Fluid of Patients with Pulmonary Tuberculosis
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摘要: [摘要] 目的 检测肺结核患者肺泡灌洗液(bronchoalveolar lavage fluid,BALF)及外周血(peripheral blood,PB)中T淋巴细胞亚群,探讨其在肺结核诊断中的价值.方法 选取47例肺结核患者(观察组)及21例健康体检者(对照组)为研究对象,运用流式细胞仪检测其BALF及外周血中T细胞亚群,并比较其差异性.结果 观察组患者BALF及外周血中CD4 比例及CD4/CD8比值均明显低于对照组(P<0.05);观察组患者BALF中CD8比例显著高于外周血(P<0.05).肺结核患者经治疗2个月后,BALF及外周血中CD4 比例及CD4/CD8比值均有明显升高,BALF中CD8水平与治疗前相比明显降低(P<0.05).绘制ROC曲线比较显示,BALF中T淋巴细胞亚群检测价值高于外周血,差异有统计学意义(P<0.05).结论 BALF及外周血中T淋巴细胞亚群检测有助于肺结核的诊断及观察疗效.Abstract: [Abstract]Objective To evaluate the clinical value of T lymphocyte subsets analysis in bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) in patients with pulmonary tuberculosis. Methods Forty-seven patients with pulmonary tuberculosis (observation group) and 21 normal persons (control group) were enrolled in this study. T lymphocyte subsets in BALF and PB of patients were detected by flow cytometry (FCM), and the differences were compared. Results The CD4 and CD4/CD8 ratios in BALF and PB of observation group were significantly lower than control group (P<0.05), and CD8 in observation group was significantly higher in BALF than in PB (P<0.05). After 2 months' treatments, the CD4 and CD4/CD8 ratios in BALF and PB of observation group were significantly increase,and the CD8 in BALF was reduced (P<0.05). T lymphocyte subsets in BALF showed significantly higher clinical value in diagnosis of pulmonary tuberculosis than that in PB (P<0.05), after ROC curve analysis. Conclusion Analysis of T lymphocyte subsets in BALF and PB is helpful for clinic diagnosis and observation of patients with pulmonary tuberculosis.
期刊类型引用(11)
1. 薛占成,韩海燕,刘冀琴,周秀. 机化性肺炎患者T淋巴细胞亚群分析的临床意义. 实用检验医师杂志. 2023(02): 185-188 . 百度学术
2. 付春芳,赵鑫. 肺结核患者检测免疫功能指标及血清PCT、PA的临床意义探讨. 中国实用医药. 2022(10): 104-106 . 百度学术
3. 郑树斌,陈天杨,陈欣羽,刘孟珂. 肺泡灌洗液中T淋巴细胞亚群水平对于肺结核诊断和疗效判断的Meta分析. 临床医学研究与实践. 2021(01): 15-18+22 . 百度学术
4. 何晗,唐柳生,李世立,董捷,周游,韦显珍. 青年肺结核患者支气管肺泡灌洗液T淋巴细胞亚群及炎症因子测定价值分析. 右江医学. 2020(09): 686-690 . 百度学术
5. 吴雪琼. 淋巴细胞亚群检测及对结核病诊断和患者免疫状况评价的价值. 中国防痨杂志. 2020(10): 1020-1024 . 百度学术
6. 冯睿婷,赵继智. 肺结核患者血清T淋巴细胞亚群检测流式细胞术的应用. 内蒙古医学杂志. 2020(10): 1190-1191 . 百度学术
7. 张丽柯,杨卫,史芳瑜,徐全晓. 流式细胞仪淋巴亚群在肿瘤化学治疗后患者中的应用效果. 实用医技杂志. 2019(04): 445-447 . 百度学术
8. 庞凡,仲燕,刘雅. 肺结节病患者支气管肺泡灌洗液及外周血中T淋巴细胞亚群水平检测的意义. 检验医学与临床. 2019(20): 3013-3015 . 百度学术
9. 杨秀丽. 化疗联合艾迪注射液对晚期肺癌患者瘤体变化及免疫功能的影响. 中国中医基础医学杂志. 2018(02): 222-225 . 百度学术
10. 李昱,杨庚. 白细胞介素12对耐药结核大鼠模型的影响. 中国医药生物技术. 2018(01): 54-58 . 百度学术
11. 李芹,张静. 肺结节病患者T淋巴细胞亚群检测的诊断价值. 宁夏医学杂志. 2018(10): 944-945 . 百度学术
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