Abstract: ［Abstract］Objective To investigate the drug resistance of clinical isolated Pseudomonas aeruginosa（PA） and the genotypes of extended-spectrum β-lactamases（ESBLs）produced by them in Kunming. Methods 125 clinical isolates of PA were collected from 4 hospitals in Kunming and identified with VITEK-32 automated bacteria identification instrument. The Kirby-Bauer method was used for antimicrobial susceptibility to 20 kinds of antibiotics. And polymerase chain reaction（PCR）analysis was used to detect 7 kinds of ESBLs genes including TEM，SHV， OXA，PER，VEB，GES and CTX-M-1. DNA sequencing was used to detect the positive production of PCR with GenBank as control. Results The resistance rates of 125 isolated PA were high to beta-lactam antibiotics，but low to aminoglycosides antibiotics. The detection rates of ESBLs genes were all above 90% from 4 hospitals in Kunming， and 7 kinds of ESBLs genes were all detected. Among the 7 kinds of ESBLs genes，the detection rate of TEM was 76%. Conclusion The detection rates of ESBLs genes were high in Kunming，and the distribution of ESBLs genotypes was varied. TEM was a main genotype among prevalent ESBLs genotypes. For local hospitals， attention must be paid to reasonable use of antibiotics，and should strengthen laboratories associated surveillance dynamically.