Abstract: ［Abstract］Objective To analyze the relationship between premature rupture of membranes and Candida and aerobic bacteria infection， and investigate the influence of Candida and aerobic bacteria infection on pregnancy outcomes. Methods 200 pregnant women in Liuzhou maternal and child health-care hospital from Jan 2012 to Jan 2014 were enrolled in this study. They were divided into three groups，70 patients in preterm premature rupture of membrane group（PPROM group），70 patients in term premature rupture of membrane group（TPROM group） and 60 cases in normal pregnancy group. The cervical secretions were collected to detect Candida and aerobic bacteria infection. Effect of Candida and aerobic bacteria infection on pregnancy outcomes was analyzed. Results Pathogenic bacteria infection rates of PPROM group and TPROM group were higher than that of normal pregnancy group， pathogenic bacteria infection rates of PPROM group was higher than that of pathogenic bacteria infection rates of TPROM group，and the difference were all significant（P < 0.05）. The patients with Candida and aerobic bacteria infection were enrolled as infected group， and patients without Candida and aerobic bacteria infection were enrolled as uninfected group. The rates of premature birth， cesarean section， neonatal infection，low birth weight and perinatal diseases in infected group were significantly higher than those in uninfected group（P < 0.05）. Pregnancy outcomes were compared between patients with Candida infection and aerobic bacteria infection. As the results， there were no significantly differences in the rates of premature birth， cesarean section， neonatal infection， fetal distress， perinatal diseases and perinatal death（P > 0.05）， but there was a significantly difference in the rate of low birth weight between patients with Candida infection and aerobic bacteria infection（P < 0.05）. Conclusion Candida and aerobic bacteria infection are closely related to poor pregnancy outcomes of premature rupture of membranes. Examination of pathogenic microorganism and effective antibiotic treatment can reduce the rate of premature rupture of membrane and improve maternal and neonatal outcomes.