Abstract: ［Abstract］Objective To investigate the in vitro anticancer effects of the bioactive fraction AMH-T on human cancer cell lines Hela，U251，HepG2 and the dose-effect and time-effect relationship. Methods MTT assay was used to evaluate the anticancer effect of AMH-T in vitro. The experiment set the negative group（DMSO）， the positive group（DDP，3 μM）， five dosage groups of AMH-T（2， 4， 8， 16， 32 μg/mL）. The tumor inhibition rate in each group was tested by MTT assay on 24 h， 48 h and 72 h after treatment. Results After 72h treatment with 8 μg/ml of AMH-T，most Hela cells died under inverted microscope. The inhibitory ratios of AMH-T at 2， 4， 8， 16， 32 μg/mL on Hela cells were -5.54%， 2.93%， 72.14%， 74.41% and 73.86%; 3.87%， 12.50%， 54.07%， 55.27% and 51.86% on HepG2 cells， and -5.47%， 1.08%， 54.83%， 57.75% and 71.78% on U251 cells. The IC50 values against Hela， HepG2 and U251 were 11.66 μg， 22.90 μg，and 16.85 μg，respectively. Conclusions AMH-T has significant anticancer effects on Hela，HepG2 and U251 cells in a concentration and time dependent manner. Hela cell line is most sensitive to AMH-T among three cell lines.