云南白族15个常染色体STR 基因座的遗传多态性
Polymorphism Investigation on 15 STR Loci of Yunnan Bai Population
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摘要: [摘要]目的 采用PCR- STR及基因分型技术,探讨云南怒江白族群体中D3S1358、TPOX 等15个常染色体STR基因座的遗传多态性, 建立怒江白族群体的遗传学基础数据, 为法医学应用提供基础数据.方法 采集124 名云南怒江地区白族无关个体的静脉血, 经典有机法提取DNA, 应用PCR技术, 复合扩增D3S1358等15个常染色体基因座的短串连重复序列,扩增产物用遗传分析仪电泳荧光检测, 获得云南怒江白族15个STR基因座的等位基因频率,进行遗传多态性分析.结果 15个常染色体STR基因座在云南怒江白族群体中具有遗传多态性,基因型分布符合Hardy- Weinberg平衡定律(P > 0.05). 其累积匹配概率(CPM )为4.869 1×10-17, 累积非父排除率( CPE)为99.99% ,累积个人识别能力( CDP) 为99.99%.结论 云南怒江白族群体15个常染色体STR基因座有较高的非父排除率和个体识别能力, 可为法医学亲子鉴定和个体识别提供基础数据以及遗传学研究提供依据.
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关键词:
- [关键词]聚合酶链反应 /
- 遗传多态性 /
- 短串连重复序列 /
- 怒江白族群体
Abstract: [Abstract]Objective The study aimed to investigate 15 autosomal loci polymorphism (such as D3S1358 and TPOX) in Nujiang Bai nationality, in order to construct genetic basic data of Bai population and provide a foundation for population study and forensic cases. Methods Venous blood was collected from 124 unrelated Bai individuals in Nujiang, Yunnan province. DNA was extracted by classical organic solvent extraction. PCR technique was applied to amplify the sequence of autosomal loci. The products were measured by electrical fluoroscopy. The gene frequencies of 15 STR loci were investigated and genetic polymorphisms were analyzed. Results Gene polymorphisms of 15 STR loci were detected and the distributions of genotype conformed with Hardy-Weinberg balance law(P > 0.05). The cumulative match probability (CPM) was 4.869×10-17, the cumulative excluding probability(CPE) was 99.999 99%,and the combined power of discrimination(CDP) was 99.999 97%. Conclusion The 15 STR loci used in this study were highly polymorphic informative content in Bai population and could be applied to population study and forensic practice.
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