Abstract: ［Abstract］Objective To investigate the application effect of the puncture method of indwelling needle by single hand in clinical attendance of pediatrics. Methods From March 2015 to September 2015， 578 patients in our pediatrics were randomly and equally divided into two groups. The experiment group were treated with the puncture method of indwelling needle by single hand and the control group received traditional puncturing method. Results There were statistical significances in the success rate of first puncture，degree of comfort， sense of pain， dropping of the indwelling needle，phlebitis，exosmosis of drugs and indwelling time of patients between the two groups（P < 0.05）. Conclusion In the process of using indwelling needle in pediatrics， the application of the puncture method of indwelling needle by single hand could improve the success rate of first puncture of venous retention needle，enhance the comfort degree of neonates，relieve the sense of pain， reduce the dropping of the indwelling needle，incidence of phlebitis and exosmosis of drugs，and prolong the indwelling time. The operation of this puncture is simple and easy to do and to learn，without any special equipment. In the venous puncture， the only necessities are 24G retention needle，3M indwelling needle and adhesive tape. Obviously，it is economical and practical， and is suitable for application in clinic.