Abstract: ［Abstract］Objective　To establish the resist-fatigue animal test through using Maca， in order to provide reference for the relevant departments to exploit new sports nutrition . Methods Thirty male KM mice were randomly divided into Quiet control group（A），Exhaustive group（B，high altitude），Maca exhaustive group（C）， there were 10 mice in each group. Drinking water containing Maca（Maca =500 mg/L）was supplied in group C. Additional exhaustive group（D，low altitude）was set．Thirty days later， all mice were killed after one exhaustive test， then we tested the exhaustive time，the level of T-SOD and liver glycogen. Results The exhaustive time of group C was significantly longer than group B（P＜0.05）， the T-SOD level of group C was significantly higher than group A and group B（P＜0.05）， and the liver glycogen content of group C was significantly higher than group B（P＜0.05）. Conclusion Maca can raise T-SOD and liver glycogen level of the mice after exhaustive swimming， and has function of resist-fatigue.