Abstract: ［Abstract］Objective　To study the clinical effect of radical cystectomy and orthotopic urinary diversion with 3D laparoscopy. Methods　We adopted 3D laparoscopy to do 6 cases of radical cystectomy and orthotopic urinary diversion from September 2014 to November 2015. After creating the channels，we isolated the ureter first， after ligating the branches of internal iliac artery we did the pelvic lymph node dissection，cut off the lateral bladder ligaments， lateral prostate vascular pedicles and dorsal vein complex， then reconstructed the new bladder and anastomose. Results　The operation time was 290 ~ 350 min，in which the radical surgery time was 145 ~ 170 min and the bleeding was 40 ~ 150 ml without blood transfusion. No serious complications such as pelvic vascular injury and rectal injury occurred during operation. No intestinal anastomotic fistula， the new bladder fistula， intestinal obstruction， and wound infection occurred after the surgery. Conclusions　Three-dimensional imaging completed by the 3D laparoscopy can effectively eliminate the vision disparity compared with 2D laparoscopy， and make the operation more accuracy. Therefore it can effectively shorten the operation time and learning curve.