三种外阴阴道假丝酵母菌病致病菌种鉴定方法比较

李春美

三种外阴阴道假丝酵母菌病致病菌种鉴定方法比较

李春美

(昆明医科大学第一附属医院产科,云南昆明 650032)

基金项目: ［基金项目］国家自然科学基金资助课题（8110076）

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出版历程

Comparison of Three Identification Tests of Vulvovaginal Candidiasis' Candida Strains

Li Chun Mei

(Dept. of Gynecology and Obstetrics,The First Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)

摘要

摘要: ［摘要］目的　分析比较3种阴道假丝酵母菌病（VVC）致病菌种鉴定方法的优劣．方法　以科玛嘉假丝酵母菌显色培养基、安图显色培养基和VITEK 2系统分别进行致病假丝酵母菌菌种鉴定，最终鉴定结果以VITEK 2 系统为准．结果　共获得RVVC、VVC患者致病菌株243株，经VITEK2系统鉴定为白假丝酵母菌217株，非白假丝酵母菌26株．安图显色培养基鉴定白假丝酵母菌216株，非白假丝酵母菌13株．科玛嘉假丝酵母菌显色培养基鉴定白假丝酵母菌214株，非白假丝酵母菌16株．与VITEK2系统的鉴定符合率分别为94.7%和98.62%，2种显色培养基对白假丝酵母菌和非白假丝酵母菌的鉴定结果差异无统计学意义（P＞0.05）．结论　相对于科玛嘉假丝酵母菌显色培养基，安图显色培养基价格低，颜色辨别度高，操作更简单，更值得在临床假丝酵母菌鉴定中推广应用．
- [关键词]假丝酵母菌 /
- 鉴定 /
- 显色培养基
Abstract: ［Abstract］Objective　To compare the advantages and disadvantages of three methods of yeast-like fungi identification of vulvovaginal candidiasis（VVC） . Methods The pathogenic candida strains were identified by CHROM agar chromogeinc culture medium， the chromogenic medium of Autobio and VITEK 2 system， and the result of VITEK 2 system was the gold standard. Results All candida strains were appraised. By VITEK2 system， 217 candida isolates were identified as candida albicans，and 26 were detected as non-candida albicans. 214 isolates were identified as candida albicans and 13 isolates were identified as non-candida albicans by Autobio . 214 isolates were appraised as candida albicans，16 isolates were identified as non-candida albicans. The coincidence rate of identification of CHROMagar and the chromogenic medium of Autobio were 94.7% and 95.06%. Compared with VITEK 2 system，there was no significant difference between these two chromogeinc culture medium identifying candida albicans and non-candida albicans. Conclusion The chromogenic medium of Autobio has higher cost-effectiveness， higher color discrimination and simpler technical operation， and is worthy of promotion in identifying candida species in clinic.
- ［Key words］Candida；Identification；Chromogeinc culture medium /

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