丙泊酚对脑损伤大鼠认知功能及BDNF表达的影响

李金路

丙泊酚对脑损伤大鼠认知功能及BDNF表达的影响

李金路

(1)苏州大学附属第二医院麻醉科,江苏苏州 215004;2)昆明医科大学第一附属医院麻醉科,云南昆明 650032)

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出版历程

Effects of Propofol on Cognitive Function and BDNF Expression in Rats with Traumatic Brain Injury

Li Jin Lu

(1)Dept. of Anesthesiology,The 2nd Affiliated Hospital of Suzhou University,Suzhou Jiangsu 215004;2)Dept. of Anesthesiology,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650031,China)

摘要

摘要: ［摘要］目的　研究丙泊酚对创伤性脑损伤后大鼠认知功能以及脑皮质BDNF表达的影响，并阐明其脑保护的作用．方法　随机将训练后的48只成年SD大鼠随机分成3组．假手术组仅行手术操作，不给予改良的Feeney氏法自由落体撞击；对照组采用改良的Feeney氏法制作大鼠脑外伤模型；丙泊酚组在脑外伤模型制备后继续经尾静脉注射丙泊酚10 mL/(kg·h) 100 mg/(kg·h)，持续6 h．术后3 d和14 d对大鼠进行神经功能缺损评分（NSS评分）和Morris水迷宫试验判定大鼠神经行为学功能，取材损伤皮质分别行RT-PCR、Western blot测定脑皮质BDNF表达情况．结果　与假手术组比较，对照组术后3 d、14 d 脑NSS评分增加、逃避潜伏期延长、穿越平台次数减少均具有统计学意义（P＜0.01），而皮质BDNF表达仅在术后3 d减少（P＜0.01），术后14 d差异无统计学意义（P＞0.05）．与对照组比较，丙泊酚组脑皮质BDNF表达在术后3 d、14 d表达均增加（P＜0.01）；且术后14 d，NSS评分减少、逃避潜伏期缩短、穿越平台次数增加（P＜0.01）．结论　丙泊酚促进创伤性脑损伤大鼠神经功能和学习记忆认知功能的恢复可能与促进BDNF的表达相关．
- [关键词]丙泊酚 /
- 创伤性脑损伤 /
- BDNF /
- 认知功能
Abstract: ［Abstract］Objective　To study the effects of propofol on cognitive function and expression of BDNF in the cerebral cortex after the traumatic brain injury （TBI）. Methods　Forty-eight SD rats treated with the Morris water maze were randomly divided into 3 groups with 16 rats in each group. Rats in the sham group were subjected to the operative procedure but prevented from traumatic brain injury. Rats in the control group and the propofol group were subjected to the traumatic brain injury named Feeney. After the operation，rats in the propofol group were injected propofol for 6 hours. The neurological functional status and the cognitive function of rats were assessed with NSS scores and Morris water maze tests on 3d and 14d post operation. RT-PCR and Westeron Blot were used to detect the expressional changes of BDNF in the injured cerebral cortex．Results　Compared with the sham group， NSS scores and the latencies of escape among rats in the control group were increased and times of crossing platform were decreased at 3d and 14 d post operation（P ＜0.01），and the mRNA and protein levels of BDNF were decreased at 3d post operation （P ＜0.01）. Compared with the control group， the mRNA and protein levels of BDNF in the propofol treated rats were increased at 3d and 14d post operation （P ＜0.01）， NSS scores were lower at 14d （P ＜0.01），the latencies were shorter and the times were increased（P ＜0.01）. Conclusion　Propofol improves the the expression of BDNF in the injured cerebral cortex and facilitates the recovery of the neurological and cognitive function in rats with traumatic brain.
- ［Key words］Propofol；Traumatic brain injury；BDNF；Cognitive function /

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