Abstract: ［Abstract］Objective　To investigate the gene expression change of TNF-α， IL-6 and IL-1β at different time points in brain tissues of rats with cerebral ischemia reperfusion injury. Methods　A total of 24 adult male SD rats were randomly divided into four groups: sham group and 3 groups with brain ischemia reperfusion of 3h， 6h and 12h. Real-Time PCR was used to analyze the gene expression of TNF-α， IL-6 and IL-1β at 3h， 6h， and 12h after reperfusion. Results　In the sham group， the mRNA expression levels of TNF-α， IL-6 and IL-1βin were low， but increased immediately after brain ischemia injury and decreased gradually thereafter. The gene expression of TNF-α mRNA at 3h after reperfusion was significantly increased and reached the peak （P＜0.01） then significantly decreased at 12h after reperfusion. The gene expression of IL-6 mRNA was notably increased at 3h after reperfusion and peaked at 6h （P＜0.01）， and significantly decreased at 12h compared with 6h （P＜0.01）. The gene expression of IL-1βmRNA at 3h after reperfusion was significantly increased， peaked at 6h （P＜0.01） and significantly decreased at 12h （P＜0.01）. Conclusion　The gene expression levels of TNF-α， IL-6 and IL-1β mRNA increased significantly in the early stage of reperfusion and decreased gradually after reaching the peak， which suggested that the gene expression change of TNF-α，IL-6 and IL-1β was involved in the mechanism of cerebral ischemia reperfusion injury.