调节性T细胞培养及输入慢性阻塞性肺病大鼠后免疫细胞变化
Cuture of Regulatory T Cells and the Changes of Immune Factors in COPD Rats after Venous injection of Regulatory T Cells
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摘要: ［摘要］目的 在慢性阻塞性肺病大鼠体内输入调节性T细胞，观察其输入调节性T细胞后大鼠体内的免疫细胞变化．方法 清洁级雄性SD大鼠21只，随机分为3组，通过烟熏建立大鼠COPD模型．经磁珠分离大鼠CD4+CD25+调节性T细胞（Treg）进行培养．细胞培养成功后给COPD大鼠尾静脉输注5×104/mL、5×105/mL， 5×106/mL各1 mL．通过流式细胞仪检测细胞因子，采用ELISA检查IL-6，C反应蛋白（CRP）．结果 培养基中添加JJ316或重组人IL-2均有利于CD4+CD25+调节性T细胞生长，2种方法在培养至20 d左右均出现细胞生长停滞．培养成功的调节性T细胞以不同量输入3组烟熏COPD大鼠体内，输注1周后体内炎症因子检测提示：大鼠出现CRP，IL-6下降．结论 研究提示大鼠体内输入CD4+CD25+调节性T细胞有助于抑制COPD炎症的进展，通过提升COPD体内调节性T细胞量可能减缓COPD炎症的进展.
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关键词:
- [关键词]慢性阻塞性肺病 /
- 调节性T细胞 /
- 免疫
Abstract: ［Abstract］Objective To study the change of immune factor in COPD rats after intravenous injection of regulatory T cells. Methods Twenty-one SPF rats was divided into three groups at random， rat COPD model was built by smoking. We used magnetic bead isolation technic to separate CD4+CD25+ regulatory T cells. Regulatory T cells were cultured and injected into rats though rats' caudal vein according to different dose， 5×104/mL，5×105/mL，5×106/mL respectively. Flow cytometry was used to analyze cell factors. ELISA was used to analyze IL-6 and CRP．Results Adding JJ316 or IL-2 into medium benefited the proliferation of CD4+CD25+ regulatory T cells. On the 20 th day， regulatory T cells CD4+CD25+ proliferation stopped by adding JJ316 or IL-2 respectively. Regulatory T cells were cultured and injected into rats though rat caudal vein according to different dose. The levels of CRP and IL-6 were decreased when rats were injected by CD4+CD25+regulatory T cell after one week. Conclusions Injection of regulatory T cells is helpful to control inflammation progression of COPD， so the increase of regulatory T cells of patients with COPD may decrease inflammation progression of COPD. -
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