Abstract: ［Abstract］Objective To compare the consistency of the results of clinical specimens between Tecan Freedom Evolyzer-2200 automated enzyme immunoassay analyzer and manual ELISA method， so as to explore more scientific and reliable test method. Methods Ninety samples were randomly selected from clinical and physical examination，the IMARK2 microplate reader and the instrument operating manual sample measurement was used to test hepatitis A，hepatitis B 5， hepatitis C，hepatitis E 2，TP and HIV，and the results were given correlation and linear regression analysis. Results For the test items of HBsAg，HBsAb， HBeAg， TP， HIV， the correlation coefficient was r2> 0.9，the data showed a good correlation，and for HAV-IgM， HBeAb， HBcAb， HCV，HEV-IgM，HEV-IgG，the correlation coefficient was 0.9> r 2 > 0.6. Compared with manual method， the total coincidence rate of automated enzyme immunoassay analyzer was 96.9% in 11 indexes of 90 samples. Conclusions　Automatic and manual ELISA method has a good correlation. Automatic ELISA also greatly reduce the labor burden of laboratory personnel， improve efficiency， should be strengthened in clinical practice.