Abstract: ［Abstract］Objective To investigate the application effect of indwelling needle puncture in the axillary vein of newborn infants. Methods　From September 2015 to February 2016， the venous indwelling needle puncture was given for neonatal transfusion 180 cases， including 90 cases of the axillary vein indwelling needle infusion in the experimental group， and 90 cases of puncture of extremity vein infusion in the control group. The application effect of the 2 groups was compared. Results　There were significant differences in terms of indwelling needle shedding， phlebitis，drug extravasation， lien time between the experimental group and the control group （P < 0.05） . Conclusions During neonatal indwelling needle puncture infusion process，the axillary vein indwelling needle infusion can prevent the indwelling needle shedding， decrease the incidence of phlebitis and reduce the incidence of drug extravasation，and can prolong the indwelling time. Puncture in the simple operation easy， is easy to learn and no need for special facilities and equipment，only conventional intravenous infusion required material，plus 24g indwelling needle，aseptic transparent dressing，paper tape，economical and practical，suitable for application in neonatal ward.