宫颈癌患者治疗前检测血清HPV16E6 mRNA和CK19 mRNA的临床意义

杨琳琳; 王薇; 张磊; 张红平; 杨宏英

宫颈癌患者治疗前检测血清HPV16E6 mRNA和CK19 mRNA的临床意义

昆明医科大学第三附属医院云南省肿瘤医院妇科

基金项目:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2013FB168);

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出版历程
- 收稿日期: 2016-09-20

Clinical Significance of Examination of Pretreatment Peripheral Blood HPV16E6 mRNA and CK19 mRNA in Cervical Cancer Patients

Funds:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2013FB168);

摘要

摘要: 目的检测宫颈癌患者治疗前外周血HPV16E6m RNA和CK19m RNA的表达, 探讨其对宫颈癌的诊断意义及其与临床病理的相关性.方法对100例宫颈癌患者行治疗前外周血HPV16E6m RNA及CK19m RNA检测, 以40例健康妇女为对照, 分析其作为诊断参考的特异性和敏感性, 并对两者与宫颈癌临床病理特征的相关性进行分析.结果 HPV16E6m RNA、CK19m RNA检测诊断宫颈癌的特异性均为97.5%.HPV16E6m RNA、CK19m RNA升高用于治疗前诊断的敏感性分别为15%和39%, 两者差异有统计学意义 (P<0.05) .HPV16E6m RNA升高与临床分期 (P=0.012) 、盆腔淋巴结转移 (P=0.026) 相关.CK19m RNA升高与深肌层浸润 (P=0.031) 、盆腔淋巴结转移 (P=0.027) 相关.结论外周血HPV16E6m RNA和CK19m RNA均是宫颈癌微转移有价值的肿瘤标志物;CK19m RNA在作为宫颈癌指标时, 临床诊断价值优于HPV16E6m RNA.
- 宫颈肿瘤 /
- HPV16E6m RNA /
- CK19m RNA /
- 外周血
Abstract: Objective To investigate the significance of pretreatment peripheral blood HPV16E6m RNA and CK19m RNA in diagnosis and their correlations to the clinicopathologic features of cervical carcinoma.Methods One hundred cervical carcinoma patients underwent pretreatment peripheral blood HPV16E6m RNA and CK19m RNA evaluation;40 healthy women were subjected as control.The specificity and sensitivity of HPV16E6m RNA and CK19m RNA as diagnostic indexes were analyzed; their correlations to clinicopathologic features were investigated.Results The specificity of HPV16E6m RNA and CK19m RNA in the diagnosis of cervical cancer were 97.5%.The sensitivity of HPV16E6m RNA and CK19m RNA in the diagnosis of pre treatment were 15% and39%, respectively and the difference was statistically significant (P <0.05) .HPV16E6m RNA elevation was associated with clinical stage (P= 0.012) and pelvic lymph node metastasis (P=0.026) . CK19m RNA was associated with deep muscular invasion (P=0.031) and pelvic lymph node metastasis (P=0.027) .Conclusion Peripheral blood HPV16E6m RNA and CK19m RNA are the valuable tumor markers of cervical cancer.The value of CK19m RNA in the diagnosis of cervical cancer is better than that of HPV16E6m RNA.
- Cervical neoplasm /
- HPV16E6m RNA /
- CK19m RNA /
- Peripheral blood

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参考文献(15)

[1]	[1]QMICHOU Z, KHYATTI M, BERRAHO M, et al.Analysis of mutations in the E6 oncogene of human papillomavirus 16 in cervical cancer isolates from Moroccan women[J].BMC Infect Dis, 2013, 13 (8) :378.
[2]	[2]SHI Y l, LI L, HU Z, et al.A genome-wide association study identifies two new cervical cancer susceptibility loci at4q12 and 17q12[J].Nat Genet, 2013, 45 (8) :918-922.
[3]	[3]JIANG B, XUE M.Correlation of E6 and E7 levels in high-risk HPV16 type cervical lesions with CCL20 and Langerhans cells[J].Genet Mol Res, 2015, 14 (3) :10473-10481.
[4]	[4]LEE S J, LEE A W, KANG C S, et al.Clinicopathological implications of human papilloma virus (HPV) L1 capsid protein immunoreactivity in HPV16-Positive cervical cytology[J].Int J Med Sci, 2014, 11 (1) :80-86.
[5]	[5]TORTORA M, ANNUNZIATA C, LIGUORI G, et al.Detection of human papillomavirus DNA in peri-tumor tissues and pelvic lymph nodes as potential molecular marker of micrometastasis in cervical cancer[J].Infect Agent Cancer, 2016, 11 (1) :1-7.
[6]	[6]ZHAO S, YANG H, ZHANG M, et al.Circulating tumor cells (CTCs) detected by triple-marker Ep CAM, CK19, and h MAM RT-PCR and their relation to clinical outcome in metastatic breast cancer patients[J].Cell Biochem Biophys, 2013, 65 (2) :263-273.
[7]	[7]SUN D W, ZHANG Y Y, SUN X D, et al.Prognostic value of cytokeratin 19 in hepatocellular carcinoma:A meta-analysis[J].Clin Chim Acta, 2015, 25 (8) :161-169.
[8]	[8]ZHANG X, CHEN S B, CHEN J X, et al.CK19 m RNA expression in the bone marrow of patients with esophageal squamous cell carcinoma and its clinical significance[J].Dis Esophagus, 2010, 23 (5) :437-443.
[9]	[9]YOSHIDA K, SATO K, TONOGI M, et al.Expression of Cytokeratin 14 and 19 in Process of Oral Carcinogenesis[J].Bull Tokyo Dent Coll, 2015, 56 (2) :105-111.
[10]	[10]LIU Z, YU P, XIONG Y, et al.Significance of CK19, TPO, and HBME-1 expression for diagnosis of papillary thyroid carcinoma[J].Int J Clin Exp Med, 2015, 8 (3) :4369-4374.
[11]	[11]ZHOU L, ZHANG H, HUANG C, et al.Expression of Daxx and HPV16 E6 and its significance in cervical lesions[J].Zhonghua Yi Xue Za Zhi, 2015, 95 (37) :3050-3053.
[12]	[12]YANG X, LU L.Expression of HPV-16 E6 protein and p53 inactivation increases the uterine cervical cancer invasion[J].Drug Res, 2015, 65 (2) :70-73.
[13]	[13]OKAMOTO S, NIIKURA H, NAKABAYASHI K, et al.Detection of sentinel lymph node metastases in?cervical cancer:assessment of KRT19 m RNA in the one-step nucleic acid amplification (OSNA) method[J].Gynecol Oncol, 2013, 130 (3) :530-536.
[14]	[14]ZHANG F, LIU D, LIN B, et al.Expression of high-risk HPV DNA and CK19 in pelvic lymph nodes in stage Ia-IIa cervical cancer and their clinical value[J].Oncol Rep, 2012, 27 (6) :1801-1806.
[15]	[15]REN C, HAN C, FU D, et al.Circulating tumor cells in breast cancer beyond the genotype of primary tumor for tailored therapy[J].Int J Cance, 2016, 138 (7) :1586-1600.

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