Abstract: Objective To improve our understanding of brucellosis by analyzing its epidemiological and clinical features, laboratory examination of brucellosis in Yunnan Province.Methods Clinical and laboratory data of brucellosis patients in Qujing First People's Hospital from 2013 to 2014 were retrospectively analyzed with its relevant literature reviewed.Results A clear epidemiological history was found in all the 8 brucellosis patients, and they were manifested as fever, sweat, weakness, hepatosplenomegaly and lymphadenopathy.They got to their first appointment in all kinds of clinical departments, such as Urology, Endocrinology, respiration, infectious diseases, oncology, Hematology, neurology and other clinical departments.Among the other accompanied various symptoms and signs of the patients included fever and muscle ache, fever of unknown origin, fever with white blood cells reduction, right abdominal pain, fever accompanied cough and sputum, discontinuity headache and no obvious inducement of left testis gall pill.Cultured venous blood from 8 patients was positive for brucella.Blood white blood cell count and liver function are normal.Conclusion Brucellosis shows various clinical manifestations, and the brucellosis patients are first diagnosed at different clinical departments.So, clinical and laboratory physicians should pay more attention to brucellosis combining with the epidemiological characteristics, as early as possible to perform the blood bacterial culture for patient with long-term fever due to unknown reasons.