紫杉醇对人胆管癌QBC939细胞wnt/β-catenin信号通路基因和蛋白表达的影响

侯峰强; 董山潮; 雷喜锋; 张伟

紫杉醇对人胆管癌QBC939细胞wnt/β-catenin信号通路基因和蛋白表达的影响

渭南市中心医院普外科

基金项目:

基金：国家自然科学基金资助项目(81560238);

计量
- 文章访问数: 2147
- HTML全文浏览量: 763
- PDF下载量: 59
- 被引次数: 0
出版历程
- 收稿日期: 2016-11-14

Effects of Paclitaxel on Gene and Protein Expression of wnt/β-catenin Signaling Pathway in Cholangiocarcinoma Cell Line QBC939

Funds:

基金：国家自然科学基金资助项目(81560238);

摘要

摘要: 目的探讨紫杉醇对人胆管癌QBC939细胞wnt/β-catenin信号通路中基因和蛋白表达的影响.方法常规培养QBC939细胞,Westernblot检测0.1、1、10 nmol/L紫杉醇组和对照组细胞中β-catenin蛋白的表达量,Q-PCR检测10 nmol/L紫杉醇组和对照组细胞中P53和C-jun基因表达,干预时间均为24 h.结果与对照组比较,紫杉醇各组β-catenin蛋白含量均有下降,当紫杉醇浓度达到10 nmol/L时,β-catenin蛋白表达明显降低(P<0.05).与对照组比较,经10 nmol/L的紫杉醇干预24 h后,P53基因表达上升(P<0.05),而C-jun基因表达下降(P<0.05).结论紫杉醇通过抑制QBC939细胞内β-catenin蛋白表达,阻断Wnt信号通路,诱导抑癌基因P53的表达,降低癌基因C-jun的表达.
- 紫杉醇 /
- QBC939 /
- β-catenin /
- P53 /
- C-jun
Abstract: Objective To explore the effects of paclitaxel on gene and protein expression of wnt/β-catenin signaling pathway in cholangiocarcinoma cell line QBC939. Methods Western blot was used to detectβ-cateninprotein expression level in 0.1, 1, 10 nmol/L paclitaxel groups and control group for 24 h. q-PCR was used to detect c-jun and p53 mRNA expression level in 10nmol/L paclitaxel group and control group for 24 h.Results Compared with control group, the expression levels of β-catenin protein in paclitaxel groups were decreased, the expression levels of β-catenin protein were significantly reduced when paclitaxel concentration at10 nmol/L(P<0.05). Compared with control group,the expression levels of p53 mRNA were up-regulated and c-jun mRNA were down-regulated in10nmol/L paclitaxel group(P <0.05). Conclusions Paclitaxel may Down-regulate the expression of β-catenin and up-regulate the p53 mRNA, then block the wnt/β-catenin signaling pathway and down-regulate c-jun mRNA in cholangiocarcinoma cell line QBC939.
- Paclitaxel /
- QBC939 /
- β-catenin /
- P53 /
- C-jun

HTML全文

参考文献(15)

[1]	[1]ROWINSKY E K,ONETTO N,CANETTA R M,et al.Taxol:the first of the taxanes,an important new class of antitumor agents[J].Semin Oncol,1992,19(6):646-662.
[2]	[2]YAMASAKI M,MURAKAMI I,NAKANO K,et al.Carboplatin plus weekly paclitaxel combined with bevacizumab as first-line treatment for non-small cell Lung cancer[J].Anticancer Res,2017,37(2):923-928.
[3]	[3]KANG F C,CHEN P J,PAN B S,et al.Apoptotic effect of cordycepin combined with cisplatin and/or paclitaxel on MA-10 mouse Leydig tumor cells[J].Onco Targets Ther,2015,8(1):2345-2360.
[4]	[4]CADAMURO M,SPAGNUOLO G,SAMBADO L,et al.Low-Dose Paclitaxel Reduces S100A4 Nuclear Import to Inhibit Invasion and Hematogenous Metastasis of Cholangiocarcinoma[J].Cancer Res,2016,76(16):4775-4784.
[5]	[5]KIM S W,HASANUZZAMAN M,CHO M,et al.Role of14-3-3 sigma in over-expression of P-gp by rifampin and paclitaxel stimulation through interaction with PXR[J].Cell Signal,2017,2(31):124-134.
[6]	[6]ITO Y,KIKUCHI E,TANAKA N,et al.Down-regulation of NF kappa B activation is an effective therapeutic modality in acquired platinum-resistant bladder cancer[J].BMC Cancer,2015,4(29):324.
[7]	[7]DZIADYK J M,SUI M,ZHU X,et al.Paclitaxel-induced apoptosis may occur without a prior G2/M-phase arrest[J].Anticancer Res,2004,24(1):27-36.
[8]	[8]LI S,YANG L,WANG J,et al.Analysis of the chemotherapeutic effects of a propadiene compound on malignant ovarian cancer cells[J].Oncotarget,2016,7(35):57145-57159.
[9]	[9]YING L,ZHU Z,XU Z,et al.Cancer associated fibroblast-derived hepatocyte growth factor inhibits the paclitaxel-Induced apoptosis of lung cancer A549 cells by up-regulating the PI3K/Akt and GRP78 signaling on a microfluidic platform[J].PLo S One,2015,10(6):e0129593.
[10]	[10]ZHANG C,ZHANG Z,ZHANG S,et al.Targeting of Wnt/β-catenin by anthelmintic drug pyrvinium enhances sensitivity of ovarian cancer cells to chemotherapy[J].Med Sci Monit,2017,23(16):266-275.
[11]	[11]SHEN D Y,ZHANG W,ZENG X,et al.Inhibition of Wnt/β-catenin signaling downregulates P-glycoprotein and reverses multi-drug resistance of cholangiocarcinoma[J].Cancer Sci,2013,104(10):1303-1308.
[12]	[12]AHRENDT S A,RASHID A,CHOW J T,et al.p53 overexpression and K-ras gene mutations in primary sclerosing cholangitis-associated biliary tract cancer[J].J Hepatobiliary Pancreat Surg,2000,7(4):426-431.
[13]	[14]SUTO T,HABANO W,SUGAI T,et al.Aberrations of the K-ras,p53,and APC genes in extrahepatic bile duct cancer[J].J Surg Oncol,2000,73(3):158-163.
[14]	[15]KHAN S A,THOMAS H C,TOLEDANO M B,et al.p53Mutations in human cholangiocarcinoma:a review[J].Liver Int,2005,25(4):704-716.
[16]周彦明,朱有全,冯颖.癌基因c-Fos、c-Jun在胆系恶性肿瘤中的表达[J].中华普通外科杂志,2002,17(2):123.

相关文章(20)

[1]	邓勇军, 陈倩, 邹建彬, 宫政, 刘焕鹏. ZIC1基因过表达激活P53信号通路抑制胸膜间皮瘤细胞增殖, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20240405
[2]	陀晓宇, 李超, 郭丽萍, 赵华君. 尿AD7c-NTP与P300检查在AD源性MCI诊断的价值, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230407
[3]	赵斌, 段元鹏, 张国颖, 毕城伟, 杨李波, 施致裕, 杨勇, 张建朋, 高婷. CircRNA EZH2通过调控miR-30c-5p促进前列腺癌细胞增殖和迁移, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220731
[4]	江岩, 赵正科, 王光彦, 许连刚, 李梦蝶, 王茂繁, 龙双梅, 刘蛟, 钱晋. 血清GAD、ICA及 C-P联合检测对1型糖尿病的诊断价值, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220416
[5]	王硕, 茹琪, 张晓婵, 杨志瑜, 王乐, 李柳. MTHFR C677T联合CYP2C19基因检测在紫杉醇释放冠脉球囊导管术后应用, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220618
[6]	王硕, 孙爱, 张慧杰, 尤杨, 魏立业, 李柳. 心肌缺血预适应在紫杉醇释放冠脉球囊导管扩张术中的应用, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20211225
[7]	阎婷婷, 田丹, 李新, 俎云芬, 李崇阳, 尚学琴. MicroRNA Let-7a与p53基因在肺癌中表达的相关性, 昆明医科大学学报.
[8]	宋飞, 向盈盈, 毛志坚, 张小文. 2种不同方法制备的紫杉醇壳聚糖缓释膜的药物缓释特性对比, 昆明医科大学学报.
[9]	李静娴, 何迷, 柳瑞, 邢艺苑, 简远熙, 王家平, 赵睿敏. 多西紫杉醇联合伽马刀对肝癌细胞裸鼠皮下移植瘤凋亡作用的影响, 昆明医科大学学报.
[10]	陈楠. 培美曲塞联合顺铂与紫杉醇联合顺铂治疗晚期非小细胞肺癌的临床疗效观察, 昆明医科大学学报.
[11]	储雯. 云南白药对体外培养人牙周膜成纤维细胞C-fos、C-jun基因表达的影响, 昆明医科大学学报.
[12]	何志江. 重组人p53腺病毒注射液介入治疗原发性肝癌临床疗效观察, 昆明医科大学学报.
[13]	何迷. 多西紫杉醇在肝癌细胞裸鼠肝脏移植瘤中对P53、VEGF作用的研究, 昆明医科大学学报.
[14]	严志凌. 宿主p53基因多态性与HPV16 感染、宫颈癌发生发展的相关性, 昆明医科大学学报.
[15]	卢房利. β-catenin与视黄醇结合蛋白对糖尿病肾病的诊断价值, 昆明医科大学学报.
[16]	韩江琼. 多西紫杉醇与紫杉醇联合吡柔比星和环磷酰胺在局部进展期乳腺癌化疗中致心脏毒性的观察, 昆明医科大学学报.
[17]	吴静. 前列腺癌细胞株PC-3经Doc处理后p-c-jun和AR的表达变化, 昆明医科大学学报.
[18]	李若楠. 三七总皂苷调控c-Jun氨基末端激酶改善大鼠肝组织胰岛素抵抗的作用, 昆明医科大学学报.
[19]	胡艳丽. c-jun mRNA和c-fos mRNA在成年猫脊髓及L6背根神经节的定位, 昆明医科大学学报.
[20]	赵学英. p53基因在内质网应激诱导的晶状体上皮细胞凋亡中的作用, 昆明医科大学学报.