PAR4在食管癌细胞中的表达及其机制
The Expression and Mechanism of Protease-activated Receptor 4 in Esophageal Cancer Cells
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摘要: 目的 探讨PAR4在食管癌细胞中的表达及其机制.方法 (1)分别用PCR和蛋白印迹法检测四株细胞中PAR4 mRNA和蛋白的表达;(2)BSP法检测4株细胞中PAR4基因CpG岛的甲基化状态.结果 (1)在TE-10、TE-11、Eca-109 3株食管癌细胞中PAR4的表达明显低于正常食管上皮细胞HEEpiC;2.PAR4基因的19个CpG位点在HEEpiC、TE-10、TE-11、Eca-109细胞中的甲基化频率分别为35.4%、83.8%、87.6%、94.3%.结论 PAR4在食管癌细胞中表达降低,其表达降低可能与PAR4基因启动子区高甲基化有关.Abstract: Objective To investigate the expression of PAR4 and mechanism of esophageal cancer cells.Methods(1) We used PCR and western blot to detect the expression of PAR4 in human esophageal epithelial cells(HEEpiC), human esophageal cancer cells(TE-10, TE-11,Eca-109).(2) The BSP method was used to test the methylation of PAR4 gene promoter region in four cells.Results(1) The expression level of PAR4 mRNA in TE-10,TE-11,Eca-109 cells was lower than that of HEEpiC cells.(2) In HEEpiC,TE-10,TE-11 and Eca-109,the frequency of methoylation occurred at the 19 CpG loci of PAR4 was 35.4%,83.8%,87.6% and94.3%, respectively. Conclusion The expression of PAR4 in esophageal cancer cells is increased, the hypermethylation at promoter of PAR4 gene may contribute to less expression of PAR4 in esophageal cancer cells.
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Key words:
- PAR4 /
- Esophageal cancer cell /
- PCR /
- Western blot /
- Methylation
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