游泳运动对Fetuin-A诱导的胰岛素抵抗大鼠血清IL-2、IL-6的影响

汪君民; 吴晓虹; 王丽; 雷德鸿; 邱良武

游泳运动对Fetuin-A诱导的胰岛素抵抗大鼠血清IL-2、IL-6的影响

1. 南通大学体育科学学院
2. 昆明医科大学体育部
3. 昆明医科大学学报编辑部
4. 昆明医科大学第二临床学院

基金项目:

基金：福建省自然科学基金资助项目(2011J01151);

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出版历程
- 收稿日期: 2016-09-13

Effects of Swimming Exercise on Serum IL-2 and IL-6 of Fetuin-A-induced Insulin Resistance Rats

Funds:

基金：福建省自然科学基金资助项目(2011J01151);

摘要

摘要: 目的研究胰岛素抵抗大鼠游泳运动干预后,血清炎性细胞因子IL-2、IL-6变化情况,可以为进一步减轻和防止糖尿病的发生、发展提供科学依据.方法采用8周龄雄性SD大鼠,制备腹腔胰岛素抵抗模型:实验鼠腹腔注射纯度为95%的牛Fetuin-A,剂量为0.75 mg/kg.8 h后检测,以胰岛素抵抗指数(HOMA-IR)高低来判定造模是否成功.实验设空白对照组(C组)以及造模成功的大鼠随机分为安静对照组(RR组)和运动组(RE组)2组,每组6只.RE组给予8周无负重游泳运动,60 min/d,C组、RR组在RE组运动期间浸水随即捞出.采用免疫组化和免疫印迹法分别检测血清Fetuin-A,胰岛素抵抗指数(HOMA-IR);采用ELISA检测血清IL-2、IL-2R、IL-6含量.结果 RE组HOMA-IR显著低于RR组.RE组较RR组大鼠血清IL-2水平明显升高(P<0.05);RR组与C组比血清IL-6含量升高幅度明显;RE组与RR组比大鼠血清IL-6含量降低(P<0.01).结论游泳运动降低胰岛素抵抗指数的同时,显著提高了IL-2,改善心脏舒张功能.而IL-6降低了表达,说明IL-6也可以作为检测胰岛素抵抗参考指标.
- 游泳运动 /
- Fetuin-A /
- 胰岛素抵抗 /
- IL-2 /
- IL-6
Abstract: Objective To explore the changes of serum IL-2 and IL-6 of rats with insulin resistance after swimming training intervention,so as to provide scientific basis for further reducing and preventing the occurrence and development of diabetes. Methods Eight weeks male SD rats were induced to insulin resistance model by abdominal injection of 0.75 mg/kg of bovine Fetuin-A(purity of 95%). The insulin resistance index(HOMA IR) 8hours after injection was detected to confirm the success of modeling. The model rats were randomly divided into control group(RR) and exercise group(RE),and normal rats were selected as the blank control group(group C), 6 rats in each group. Rats in the RE group were given swimming training without weight loading,60 min/d for8 weeks, rats in group C and group RR were removed from the swimming pool immediately after flooding.Immunohistochemistry and western blot method were used respectively to detect the serum Fetuin-A and the indexes of insulin resistance(HOMA IR). ELISA was used to detect serum IL-2, IL-2R and IL-6 levels. Results The HOMA-IR of rats in RE group was significantly lower than that in RR group. Compared with the RR group,the serum IL-2 levels of rats in RE group were increased significantly(P < 0.05). Compared with the C group,the serum IL-6 levels in RR group were increased significantly. Compared with the RR group,the serum IL-6levels in RE group were decreased significantly(P < 0.01). Conclusions Swimming training can significantly improve the IL-2 levels and the diastolic function of heart at the same time of reducing insulin resistance index. And the down-regulation of IL-6 expression suggests that IL-6 may also be used as a reference to test insulin resistance index.
- Swimming exercise /
- Fetuin-A /
- HOMA-IR /
- IL-2 /
- IL-6

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参考文献(14)

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