IBS法在云南人群全同胞鉴定中的应用
Application of IBS Method in the Full Sibling Testing of Yunnan Population
-
摘要: 目的 探讨IBS法在全同胞鉴定中的实践应用情况.方法 采用PowerPlex21System试剂盒中的19个亲权鉴定中常用的常染色体STR基因座对234对确定为“全同胞”关系和200对无关个体样本的进行基因分型检测,分型结果依据《生物学全同胞关系鉴定实施规范》(2014版)计算IBS值.结果 生物学全同胞认定率为82.91%,比规范中75%的检测系统效能略高,有17.09%的样本无法给出倾向性意见;无关个体间13Abstract: Objective To investigate the practical application of IBS method in the full sibling testing.Methods PowerPlex21 System kit was used to amplify the 19 STR loci(D3S1358, D6S1043,D13S317,Penta E,D16S539,D18S51,D2S1338,CSF1 PO,Penta D,TH01, v WA, D21S11, D7S820, D5S818,TPOX,D8S1179,D12S391,D19S433,FGA) from the 234 pairs of determined full siblings and 200 unrelated individuals of Han population in Yunnan province,and ABI3130 genetic analyzer automatic detection and Gene Mapper ID v3.2 software were used for STR genotyping.The typing results were calculated on the basis of the IBS method(SF/Z JD0105002-2014).Results The rate of full siblings was 82.91%, and it was higher than 75%in the standard. 17.09% of the samples could not give tendentious opinions. The scores from 13 to 22 had 65 pairs of unrelated individuals, and it accounted for 32.50%.There were 135 pairs of less than 13 points that accounted for67.50%. Conclusion The application of IBS method in the identification of full siblings in Yunnan has a high value in forensic medicine.
-
Key words:
- IBS method /
- Fullsibing /
- Paternity test
[1]陆惠玲,刘秋玲,台运春,等.常染色体STR遗传标记在同胞鉴定中的应用[J].中国法医学杂志,2003,18(3):154-156. [2]赵书民,张素华,李成涛.采用Identifiler系统进行全同胞关系判定的准则探讨[J].法医学杂志,2011,27(1):17-21. [3]陆惠玲,周科伟,吕德坚,等.ITO法和判别函数法在同胞关系鉴定中的应用[J].法医学杂志,2009,25(2):118-122. [4]孙云龙,杨金龙,穆立伟,等.IBS法和ITO法应用于全同胞鉴定中的探讨[J].中山大学学报(医学科学版),2014,35(3):465-468. [5] 李成涛,孙宏钰,赵书民,等.生物学全同胞关系鉴定实施规范(SF/Z JD0105002-2014)[S].司法部鉴定管理局.2014-03-17. [6]刘俊宏,林源,张素华,等.同胞鉴定中风险与对策的思考[J].中国司法鉴定,2012,65(6):78-81. [7]陆惠玲,杨庆恩.用ITO法计算两个体间的血缘关系机会[J].中国法医学杂志,2002,17(3):188-191. [8]马立宇,胡利平,聂爱婷,等.云南白族人群13个快速突变(RM)Y-STR基因座多态性研究[J].昆明医科大学学报,2015,36(7):170-177. [9]胡利平,杜雷,张秀峰,等.云南汉族人群20个常染色体STR基因座遗传多态性[J].昆明医科大学学报,2016,37(5):17-21. [10]杜冰,杜宏,周斌,等.6个线粒体DNA SNP的微测序技术检测及群体遗传学研究[J].川北医学院学报,2015,30(1):15-19. [11] [11]WANG Y,LIU C,ZHANG C C,et al.Analysis of 24Y-STR haplotype data in a Chinese Han population from Guangdong Province[J].International Journal of Legal Medicine,2016,130(3):1-3. [12] [12]NISHI T,NAKAMURA T,HONDA K.Detection of a novel X-chromosomal short tandem repeat marker in Xq28 in four ethnic groups[J].Legal Medicine,2016,19(35):43-46. 点击查看大图计量
- 文章访问数: 1703
- HTML全文浏览量: 634
- PDF下载量: 104
- 被引次数: 0