NGF-BMSCs移植对阿尔茨海默病大鼠神经行为学的改善
NGF-BMSCs移植对阿尔茨海默病大鼠神经行为学的改善
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摘要:
目的 构建神经生长因子 (nerve growth factor, NGF) 基因-骨髓基质干细胞 (bone marrow stromal stem cells, BMSCs) 重组体, 将其移植入阿尔茨海默病 (alzheimer's disease, AD) 大鼠海马, 探讨AD大鼠神经行为学变化.方法 将Aβ淀粉蛋白 (β-amyloid protein) 立体定向注射入大鼠海马制备AD模型.动物随机分为5组:正常组 (Normal group) 、盐水注射组 (NS group) 、模型组 (Experiment group) 、BMSC移植组 (AD+BMSC group) 和NGF-BMSC移植组 (NGF-BMSC group) .移植2周后用Morris水迷宫检测大鼠行为学变化以评价移植效果.结果 移植BMSC和NGF-BMSC后, AD大鼠神经学学习、记忆能力比模型组显著提高, 而以后者效果更为显著 (分别为P<0.05和P<0.01) .结论 NGF基因修饰的BMSC可作为种子细胞传送NGF, 对大鼠AD的神经行为学起到显著的改善作用.
Abstract:目的 构建神经生长因子 (nerve growth factor, NGF) 基因-骨髓基质干细胞 (bone marrow stromal stem cells, BMSCs) 重组体, 将其移植入阿尔茨海默病 (alzheimer's disease, AD) 大鼠海马, 探讨AD大鼠神经行为学变化.方法 将Aβ淀粉蛋白 (β-amyloid protein) 立体定向注射入大鼠海马制备AD模型.动物随机分为5组:正常组 (Normal group) 、盐水注射组 (NS group) 、模型组 (Experiment group) 、BMSC移植组 (AD+BMSC group) 和NGF-BMSC移植组 (NGF-BMSC group) .移植2周后用Morris水迷宫检测大鼠行为学变化以评价移植效果.结果 移植BMSC和NGF-BMSC后, AD大鼠神经学学习、记忆能力比模型组显著提高, 而以后者效果更为显著 (分别为P<0.05和P<0.01) .结论 NGF基因修饰的BMSC可作为种子细胞传送NGF, 对大鼠AD的神经行为学起到显著的改善作用.
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