MAOA μ-VNTR多态性荧光标记自动分型方法的建立及应用

姚金勇; 胡利平; 聂爱婷; 黄仁武; 邱建波; 张秀峰; 饶旼; 聂胜洁

MAOA μ-VNTR多态性荧光标记自动分型方法的建立及应用

1. 红河州公安局
2. 昆明医科大学法医学院
3. 昆明医科大学司法鉴定中心
4. 南涧县公安局

基金项目:

基金：国家自然科学基金资助项目 (31660310); 云南省教育厅科学研究基金资助项目 (2015J052);

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出版历程
- 收稿日期: 2017-03-09

Establishment and Application An Automatic Typing Method for MAOA μ-VNTR Polymorphism Test using Fluorescence Labeling Techniques

Funds:

基金：国家自然科学基金资助项目 (31660310); 云南省教育厅科学研究基金资助项目 (2015J052);

摘要

摘要: 目的应用毛细管电泳和荧光标记技术, 建立MAOA μ-VNTR多态荧光标记自动分型的方法.方法使用Chelex-100法提取DNA后, 采用荧光标记和毛细管电泳检测技术对720份云南汉族无关个体血样进行MAOA μ-VNTR基因分型.结果荧光毛细管电泳分型方法能准确、高效地对MAOA μ-VNTR多态性进行分型检测.结论 MAOA μ-VNTR多态性荧光标记自动分型方法操作简便, 在针对MAOA μ-VNTR多态性的相关研究中具有良好的应用前景.
- MAOA μ-VNTR /
- 多态性 /
- 荧光标记引物 /
- 自动分型
Abstract: Objective To establish an automatic typing method for MAOA μ-VNTR polymorphism test using capillary electrophoresis and fluorescence labeling techniques. Me thods DNAwas extracted by Chelex-100 method. Blood samples from 720 unrelated Chinese Han individuals in Yunnan were genotyped by capillary electrophoresis and fluorescence labeled primer. Re s ults MAOA μ-VNTR polymorphism fluorescent labeling automatic typing method had high efficiency. And the results from this method were accurate and reliable.Conclus ions The operation of MAOA μ-VNTR polymorphism fluorescent labeling automatic typing method is simple. It has good prospects in the research for the MAOA μ-VNTR polymorphism test.
- MAOA μ-VNTR /
- Polymorphism /
- Fluorescence labeled primers /
- Automatic typing

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参考文献(12)

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