顺铂耐药卵巢癌的差异表达基因和信号通路富集分析

胡万芹; 赵洪波; 梁宏; 赵庆华; 杨丽华

顺铂耐药卵巢癌的差异表达基因和信号通路富集分析

1. 昆明医科大学第二附属医院妇产科
2. 昆明医科大学分子临床医学研究院

基金项目:

基金：云南省卫生和计划生育委员会医学学科带头人培养项目 (D-201633); 国家自然科学基金资助项目 (81360336);

计量
- 文章访问数: 2420
- HTML全文浏览量: 890
- PDF下载量: 274
- 被引次数: 0
出版历程
- 收稿日期: 2017-04-13

Analysis of Differential Expression Genes and Signal Pathways Associated with Cisplatin Resistant Ovarian Cancer

Funds:

基金：云南省卫生和计划生育委员会医学学科带头人培养项目 (D-201633); 国家自然科学基金资助项目 (81360336);

摘要

摘要: 目的分析顺铂耐药卵巢癌的差异表达基因和信号通路.方法用美国国立信息中心NCBI的GEO数据库获得顺铂耐药卵巢癌基因集GSE15372, 通过R与Bioconductor软件进行差异表达基因分析;再利用DAVID在线工具对顺铂耐药卵巢癌差异表达基因进行GO本体分析、KEGG通路分析.结果差异倍数在2倍以上、FDR值小于0.05为标准, 筛选出上调差异表达基因和下调差异表达基因获得差异表达基因211个, 其中上调基因120个, 下调基因91个;基因富集分析发现差异表达基因集中在黏着斑、TGF-β信号通路等生物过程 (P<0.05) .结论顺铂耐药卵巢癌存在一些特异的差异表达基因, 有部分信号通路在顺铂耐药卵巢癌中明显富集.
- 基因表达谱 /
- 顺铂耐药卵巢癌 /
- 差异表达基因 /
- 富集分析
Abstract: Objective To analyze the differentially expressed genes and signaling pathways in cisplatin resistant ovarian cancer. Me thods Cisplatin resistant ovarian cancer gene set GSE15372 was obtained from the GEO database of the national information center of the United States. The Differential expression gene analysis was performed by R and Bioconductor software, the GO and KEGG pathway of the differentially expressed genes were analyzed by DAVID online tool. Results 211 differentially expressed genes were obtained, including 120 up-and91 down-regulated genes as the standa rd was set that fold change was more than 2 times and FDR was less than0.05. Gene enrichment analysis of differentially expressed genes found in focal adhesion, TGF-beta signaling pathways and other biological processes (P<0.05) . Conclus ion There are some specific differentially expressed genes in the cisplatin resistant ovarian cancer, partial signal pathways were obvious enrichment in the cisplatin resistant ovarian cancer.
- Gene expression profile /
- Cisplatin resistant ovarian cancer /
- Differentially expressed genes /
- Enrichment analysis

HTML全文

参考文献(15)

[1]	[1]SIEGEL R, MA J, ZOU Z, et al.Cancer statistics[J].CA Cancer J Clin, 2014, 64 (1) :9-29.
[2]	[2]DASARI S.Tchounwou PB2.Cisplatin in cancer therapy:molecular mechanisms of action[J].Eur J Pharmacol, 2014, 5 (74) :364-378.
[3]	[3]LIU X, GAO Y, LU Y, et al.Oncogenes associated with drug resistance in ovarian cancer[J].Cancer Res Clin Oncol.2014, 10 (5) :1432-1435.
[4]	[4]TANYA BARRETT, DENNIS B, TROUP, STEPHEN E, et al.NCBI GEO:archive for high-throughput functional genomic data[J].Nucleic Acids Research, 2009, 37 (Databae issue) :885-890.
[5]	[5]SHERMAN B T, HUANG DA W, TAN Q, et al.DAVID Knowledgebase:a gene-centered database integrating heterogeneous gene annotation resources to facilitate high-throughput gene functional analysis[J].BMC Bioinformatics, 2007, 2 (8) :42.
[6]	[6]LI M, BALCH C, MONTGOMERY J S, et al.Integrated analysis of DNA methylation and gene expression reveals specific signaling pathways associated with platinum resistance in ovarian cancer[J].BMC Med Genomics, 2009, 8 (2) :34.
[7]	[7]SUBRAMANIAN A, KUEHN H, GOULD J, et al.GSEA-P:a desktop application for gene set enrichment analysis[J].Bioinformatics, 2007, 23 (23) :3251-3253.
[8]	[8]TAKAHASHI M, SUNG B, SHEN Y, et al.Boswellic acid exerts antitumor effects in colorectal cancer cells by modulating expression of the let-7 and mi R-200 micro RNA family[J].Carcinogenesis, 2012, 33 (12) :2441-2449.
[9]	[9]HUANG D, KHOE M, BEFEKADU M, et al.Focal adhesion kinase mediates cell survival via NF-kappa B and ERK signaling pathways[J].Am J Physiol Cell Physiol, 2007, 92 (4) :1339-1352.
[10]	[10]PARK H, HAN I, KWON H J, et al.Focal adhesion kinase regulates syndecan-2-mediated tumorigenic activity of HT1080 fibrosarcoma cells[J].Cancer Res, 2005, 65 (21) :9899-9905.
[11]	[11]MZLEAN G W, CARRAGHER N O, AVIZIENYTE E, et al.The role of focal-adhesion kinase in cancer.A new therapeutic opportunity[J].Nature Rev Cancer, 2005, 5 (7) :505.
[12]	[12]LONN P, MOREN A, RAJA E, et al.Regulating the stability of TGFbeta receptors and Smads[J].Cell Res, 2009, 19 (6) :21-35.
[13]	[13]PICKUP M, NOVITSKIY S, MOSES H L.The roles of TGFβin the tumour microenvironment[J].Nat Rev Cancer, 2013, 13 (5) :788-799.
[14]	[14]CHENFENGCHEN, KONG-NANZHAO, PAULP.MASC I, et al.TGFβisoforms and receptors m RNA expression in breast tumours:prognostic value and clinical implications[J].BMC Cancer, 2015, 15:1010-1012.
[15]	[15]JAY PILROSE, PHILLIP, WUL FRIDGE, et al.Decitabine reactivated pathways in platinum resistant ovarian cancer[J].Oncotarget, 2014, 5 (11) :3579-3589.

相关文章(20)

[1]	赵丽珠, 董莹, 邓玥, 杨丽华. 基于单细胞测序技术分析上皮细胞相关基因与卵巢癌患者预后的关系, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20240402
[2]	王兴粉, 邓玥, 杨丽华. 卵巢癌脂质代谢相关基因预后模型的构建及免疫浸润分析, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20240403
[3]	周仪, 米鍇, 冯晓丽. 鳜miR-25的时空表达特征及其靶向核心生物钟基因的预测分析, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20231010
[4]	廉阳秧, 岳红萍, 端娅, 胡红文, 罗芳. miRNA-15a/16调控Bmi-1蛋白在卵巢癌顺铂化疗耐药中的作用, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20231204
[5]	刘秋燕, 熊伟, 李程. 鼻咽癌同期放化疗抵抗细胞株与母细胞株lncRNA的差异表达谱分析, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20221108
[6]	徐丽秀, 美力班·吐尔逊, 克热曼·牙库甫. miR-181a在卵巢癌细胞中对顺铂的耐药作用, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220131
[7]	邓玥, 邹丹, TounaAbdelkerim Barh, 杨丽华. 基于Oncomine 数据库研究PDE4D基因在卵巢癌中的表达及血根碱的调控作用, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220303
[8]	苗文清, 王宇, 赵晓丽, 田倪妮, 尤丽英. 冠心病急性心肌梗死患者外周血差异基因表达分析及功能, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20220614
[9]	徐彤, 马岩团进, 张宇航, 何秋月, 黄薇, 吕梦欣, 唐健, 何建萍, 蒋国庆, 钱源. 不同浓度维生素D 刺激下体外滋养细胞的二代测序研究, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20221021
[10]	魏韩笑, 张爱君, 李强, 金培生. 血管内皮祖细胞外泌体调控骨髓间充质干细胞基因表达谱芯片, 昆明医科大学学报.
[11]	张璐, 吕乐春, 吴艳瑞, 袁方, 唐文羽, 邹显强, 李春银, 赵敏. 基于GEO数据库进行人类皮肤芯片核苷酸切除修复基因XPA表达的差异性, 昆明医科大学学报.
[12]	鲁晓东, 卢房利, 段钊, 杜联江. TTF-1和PTEN基因在子宫内膜癌早期病变中的表达意义, 昆明医科大学学报.
[13]	胡玉崇, 陆景坤, 崔梦瑶. PTEN及Caspase-3可能参与卵巢癌铂类耐药的机制, 昆明医科大学学报.
[14]	杨静. 顺铂对TC-1细胞表面PD-L1表达的影响及机制, 昆明医科大学学报.
[15]	杨涵. Bmi-1基因在不同类型上皮性卵巢癌组织中的表达及临床意义, 昆明医科大学学报.
[16]	焦扬. 羊水干细胞体外培养早期和后期基因表达谱分析, 昆明医科大学学报.
[17]	李恒. 非小细胞肺癌XRCC1Arg399Gln基因分析与顺铂疗效的关系, 昆明医科大学学报.
[18]	. XAGE-1基因在胃癌组织中的表达及其临床意义分析, 昆明医科大学学报.
[19]	徐本峰. XAGE-1 基因在胃癌组织中的表达及其临床意义分析, 昆明医科大学学报.
[20]	刘健刚. 骨桥蛋白基因在两种颅咽管瘤中的表达差异及意义, 昆明医科大学学报.