神经病理性疼痛老年大鼠海马突触长时程抑制的变化

魏辉明; 麻伟青; 董发团; 李文锋; 普俊杰; 贺焱峰

神经病理性疼痛老年大鼠海马突触长时程抑制的变化

解放军昆明总医院麻醉科

基金项目:

基金：成都军区“十二五”医学科研项目 (B14013);

计量
- 文章访问数: 2271
- HTML全文浏览量: 744
- PDF下载量: 82
- 被引次数: 0
出版历程
- 收稿日期: 2017-03-12

Changes in Hippocampus Synapses Caused by Long-term Depression in Old Rats with Neuropathic Pain

Funds:

基金：成都军区“十二五”医学科研项目 (B14013);

摘要

摘要: 目的探讨神经病理性疼痛老年大鼠海马CA1区突触长时程抑制 (LTD) 的变化.方法老年雄性Wistar大鼠15只, 随机均分为3组 (n=5) :假手术组 (S组) 、神经病理性疼痛模型组 (NP组) 、NP+AP5组 (NA组) .采用结扎L4, L5右侧脊神经的方法制备神经病理性疼痛模型.于模型制备后7 d、14 d和21 d观察大鼠痛行为学及足部形态, 于模型制备前 (基础值) 、制备后7 d、14 d和21 d时测定痛阈;于最后一次痛阈测定结束后3 d记录海马CA1区兴奋性突触后膜电位 (EPSP) , 以低频刺激 (LFS) 诱发LTD.NA组LFS前20 min经侧脑室给予输注AP5 (NMDA特异性拮抗剂) 6μL (11.8μg) .结果与S组比较, NP组和NA组各时点痛阈降低, NP组各时段LTD减小 (P<0.05) ;S组与NA组相比LTD差异无统计学意义 (P>0.05) .结论结扎L4, L5右侧脊神经所致的神经病理性疼痛可易化老年大鼠海马CA1区突触LTD;机制可能与NMDA受体活化相关.
- 神经痛 /
- 长时程抑制 /
- 海马 /
- 大鼠 /
- 老年
Abstract: Objective To investigate the changes in Hippocampus synapses caused by long-term depression (LTD) in old rats with neuropathic pain. Method Fifteen old male Wistar rats were randomly divided into 3 groups (n = 5 each) : sham operating group (group S) , neuropathic pain group (group NP) , and NP + AP5 group (group NA) . Neuropathic pain was produced by ligation of the right L4, L5 spinal nerve. The behaviour of the rats was evaluated. The pain threshold was measured by electric stimulation at 7, 14 and 21 d after surgery. The excitatory post-synaptic potential (EPSP) in hippocampal CA1 region was recorded at 3 d after the last measurement of pain threshold. The changes in EPSP and input/output curve and LTD induced by low frequency stimulation (LFS) of the CA1 stratum radiatum of the rat hippocampus were observed using stereotaxic technology and extracellular recording respectively. Group NA received infusion of AP5 (NMDA receptor antagonist) 6 μl by i.c.v 20 min before LFS. Results The pain threshold was significantly lower in group NP and NA than in group S.The amplitude of LTD was significantly lower in group NP than in group S and NA (P<0.05) , but there was no significant difference in the amplitude of LTD between group S and NA (P>0.05) . Conclusion The results suggest that the neuropathic pain by ligation of the right L4, L5 spinal nerve might facilitate LTD induction in the CA1 area of the hippocampus of old rats; mechanism might be related to the activation of NMDA receptors.
- Neuralgia /
- Long-term depression /
- Hippocampus /
- Rat /
- Aged

HTML全文

参考文献(15)

[1]	[1]DURIC V, MCCARSON K E.Persistent pain produces stress-like alterations in hippocampal neurogenesis and gene expression[J].J Pain, 2006, 7 (8) :544-555.
[2]魏辉明, 麻伟青, 李治贵, 等.NR2B在神经病理性痛大鼠海马突触长时程增强易化中的作用[J].中华麻醉学杂志, 2009, 29 (9) :789-792.
[3] 魏辉明, 麻伟青, 王慧明, 等.神经病理性痛大鼠海马突触长时程增强的变化[J].中华麻醉学杂志, 2008, 28 (10) :895-897.
[4]麻伟青, 魏辉明, 董发团, 等.腰4/5脊神经结扎大鼠慢性神经病性痛模型的建立[J].昆明医学院学报, 2010, 31 (6) :57-60.
[5]	[5]RODGERS R J.Effects of nicotine, mecamylamine, and hexamethonium on shock induced fighting, pain reactivity, and locomotor behaviour in rats[J].Psychopharmacology, 1979, 66 (1) :93-98.
[6]	[6]WANG M, YANG Y, DONG Z, et al.NR2B-containing N-methyl-D-aspartate subtype glutamate receptors regulate the acute stress effect on hippocampal long-term potentiation/long-term depression in vivo[J].Neuroreport, 2006, 17 (12) :1343-1346.
[7]	[7]SOUZA A, DUSSAN-SARRIA J A, MEDEIROS L F, et al.Neonatal hypoxic-ischemic encephalopathy reduces c-Fos activation in the rat hippocampus:Evidence of a long-lasting effect[J].Int J Dev Neurosci, 2014, 38 (9) :213-222.
[8]	[8]PERCIE DU SERT N, RICE AS.Improving the translation of analgesic drugs to the clinic:Animal models of neuropathic pain[J].Br J Pharmacol, 2014, 171 (12) :2951-2963.
[9]	[9]JEAN-MARC G, MICHAEL B, ZHANG M, et al.Neuronal changes induced by Varicella Zoster Virus in a rat model of postherpetic neuralgia[J].Virology, 2015, 482 (3) :167-180.
[10]	[10]SANDKUHLER J.Learning and memory in pain pathways[J].Pain, 2000, 88 (2) :113-118.
[11]	[11]DONG Z, ZHONG W, TIAN M, et al.Stress evoked by opiate withdrawal facilitates hippocampal LTP in vivo[J].Hippocampus, 2006, 16 (12) :1017-1025.
[12]	[12]GILBERT M E, MACK C M.Field potential recordings in dentate gyrus of anesthetized rats:stability of baseline[J].Hippocampus, 1999, 9 (3) :277-287.
[13]	[13]ZHUO M.A synaptic model for pain:long-term potentiation in the anterior cingulate cortex[J].Mol Cells, 2007, 23 (3) :259-271.
[14]	[14]KERVERN M, SILVESTRE DE FERRON B, ALAUXCANTIN S, et al.Aberrant NMDA-dependent LTD after perinatal ethanol exposure in young adult rat hippocampus[J].Hippocampus, 2015, 25 (8) :912-923.
[15]	[15]BHOURI M, FARROW P A, MOTEE A, et al.m Glu1 Receptor-induced LTD of NMDA receptor transmission selectively at schaffer collateral-CA1 synapses mediates metaplasticity[J].J Neurosci, 2014, 34 (36) :12223-12229.

相关文章(20)

[1]	张雪, 陈伟伟, 李翠花, 曹海涛. 基于力学理念的康复训练联合Ilizarov技术矫形对老年膝骨关节炎患者膝关节稳定性和下肢负重的影响, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230326
[2]	彭煜航, 李焱娟, 曲婧红, 唐代欢, 张宏伟, 吴睿, 徐世莲. 大鼠伏核甘丙肽镇痛作用及其机制, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230925
[3]	朱婷娜, 曹媛媛, 张园, 刘鹏亮, 王一航, 吴亚梅, 李利华, 赵永娜, 洪仕君. 不同剂量天麻素对甲基苯丙胺依赖CPP大鼠海马中TLR4/MyD88/NF-κB炎症信号通路的影响, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230226
[4]	张园, 朱婷娜, 曹媛媛, 刘鹏亮, 王一航, 吴亚梅, 李利华, 赵永娜, 洪仕君. TLR4/MyD88/NF-κB 信号通路对甲基苯丙胺依赖CPP大鼠海马的影响, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230225
[5]	刘亚南, 杨双, 彭煜航, 陈静, 徐世莲. 针刀疗法对神经痛大鼠的镇痛作用研究, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20231009
[6]	张盛庆宇, 舒逍, 吴锡南, 李志强, 武慧欣, 张媛, 蒋玉融, 杨思佳, 木云珍. 1 800 MHz射频电磁场暴露对大鼠海马GFAP、NCAM和GABA受体表达的影响, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210939
[7]	朱婷娜, 刘鹏亮, 董文娟, 于浩, 吴亚梅, 黄兆奎, 洪仕君, 赵永娜. 不同剂量天麻素对甲基苯丙胺依赖大鼠条件位置偏爱及海马小胶质细胞激活的影响, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210502
[8]	郭小兵, 吴德野, 李恒希, 吴海鹰, 张云茜, 于建云, 李坪. 一种轻型脑损伤后大鼠海马EP2的表达变化, 昆明医科大学学报.
[9]	李俊彦. 七叶皂苷对体外培养大鼠神经元生长的作用及其与BDNF的关系, 昆明医科大学学报.
[10]	王慧明. 地佐辛腹腔内注射减轻神经病理性疼痛大鼠的痛行为研究, 昆明医科大学学报.
[11]	王慧明. 地佐辛腹腔内注射减轻神经病理性疼痛大鼠的痛行为研究, 昆明医科大学学报.
[12]	李燕玲. 神经内科老年住院患者坠床原因分析及预防对策, 昆明医科大学学报.
[13]	李燕玲. 神经内科老年住院患者坠床原因分析及预防对策, 昆明医科大学学报.
[14]	王慧明. 氯胺酮对神经病理性疼痛大鼠的影响, 昆明医科大学学报.
[15]	. 小剂量氯胺酮治疗慢性顽固性灼性神经痛1例报道, 昆明医科大学学报.
[16]	. 成年小鼠海马神经细胞培养及其鉴定, 昆明医科大学学报.
[17]	唐茂丹. 大鼠视神经切断后视网膜Muller细胞及小胶质细胞变化特点, 昆明医科大学学报.
[18]	周磊. 高温对大鼠胚胎神经管发育畸形中BDNF及其受体mRNA表达变化的影响, 昆明医科大学学报.
[19]	殷露玮. SD大鼠脊髓全横断损伤后神经细胞凋亡的研究, 昆明医科大学学报.
[20]	杨金伟. 神经干细胞与嗅鞘细胞移植对脊髓全横断大鼠后肢运动功能的影响, 昆明医科大学学报.