神经病理性疼痛老年大鼠海马突触长时程抑制的变化

魏辉明; 麻伟青; 董发团; 李文锋; 普俊杰; 贺焱峰

神经病理性疼痛老年大鼠海马突触长时程抑制的变化

解放军昆明总医院麻醉科

基金项目:

基金：成都军区“十二五”医学科研项目 (B14013);

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出版历程
- 收稿日期: 2017-03-12

Changes in Hippocampus Synapses Caused by Long-term Depression in Old Rats with Neuropathic Pain

Funds:

基金：成都军区“十二五”医学科研项目 (B14013);

摘要

摘要: 目的探讨神经病理性疼痛老年大鼠海马CA1区突触长时程抑制 (LTD) 的变化.方法老年雄性Wistar大鼠15只, 随机均分为3组 (n=5) :假手术组 (S组) 、神经病理性疼痛模型组 (NP组) 、NP+AP5组 (NA组) .采用结扎L4, L5右侧脊神经的方法制备神经病理性疼痛模型.于模型制备后7 d、14 d和21 d观察大鼠痛行为学及足部形态, 于模型制备前 (基础值) 、制备后7 d、14 d和21 d时测定痛阈;于最后一次痛阈测定结束后3 d记录海马CA1区兴奋性突触后膜电位 (EPSP) , 以低频刺激 (LFS) 诱发LTD.NA组LFS前20 min经侧脑室给予输注AP5 (NMDA特异性拮抗剂) 6μL (11.8μg) .结果与S组比较, NP组和NA组各时点痛阈降低, NP组各时段LTD减小 (P<0.05) ;S组与NA组相比LTD差异无统计学意义 (P>0.05) .结论结扎L4, L5右侧脊神经所致的神经病理性疼痛可易化老年大鼠海马CA1区突触LTD;机制可能与NMDA受体活化相关.
- 神经痛 /
- 长时程抑制 /
- 海马 /
- 大鼠 /
- 老年
Abstract: Objective To investigate the changes in Hippocampus synapses caused by long-term depression (LTD) in old rats with neuropathic pain. Method Fifteen old male Wistar rats were randomly divided into 3 groups (n = 5 each) : sham operating group (group S) , neuropathic pain group (group NP) , and NP + AP5 group (group NA) . Neuropathic pain was produced by ligation of the right L4, L5 spinal nerve. The behaviour of the rats was evaluated. The pain threshold was measured by electric stimulation at 7, 14 and 21 d after surgery. The excitatory post-synaptic potential (EPSP) in hippocampal CA1 region was recorded at 3 d after the last measurement of pain threshold. The changes in EPSP and input/output curve and LTD induced by low frequency stimulation (LFS) of the CA1 stratum radiatum of the rat hippocampus were observed using stereotaxic technology and extracellular recording respectively. Group NA received infusion of AP5 (NMDA receptor antagonist) 6 μl by i.c.v 20 min before LFS. Results The pain threshold was significantly lower in group NP and NA than in group S.The amplitude of LTD was significantly lower in group NP than in group S and NA (P<0.05) , but there was no significant difference in the amplitude of LTD between group S and NA (P>0.05) . Conclusion The results suggest that the neuropathic pain by ligation of the right L4, L5 spinal nerve might facilitate LTD induction in the CA1 area of the hippocampus of old rats; mechanism might be related to the activation of NMDA receptors.
- Neuralgia /
- Long-term depression /
- Hippocampus /
- Rat /
- Aged

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参考文献(15)

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