四聚乙醛在亚急性中毒大鼠部分组织中的分布

李鸿; 郑洪富; 解润芳; 李树华; 张瑞林; 王蕊; 李慧方洁

四聚乙醛在亚急性中毒大鼠部分组织中的分布

1. 曲靖市公安局刑侦支队
2. 昆明医科大学法医学院

基金项目:

基金：国家自然科学基金资助项目 (81460213); 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FB007);

计量
- 文章访问数: 2180
- HTML全文浏览量: 888
- PDF下载量: 53
- 被引次数: 3
出版历程
- 收稿日期: 2017-04-21

Distribution of Metaldehyde in Some Tissues of Subacutely Poisoned Rats

Funds:

基金：国家自然科学基金资助项目 (81460213); 云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2014FB007);

摘要

摘要: 目的建立生物检材中四聚乙醛气相色谱/质谱以及气相色谱-FID的分析方法, 考察四聚乙醛在亚急性中毒大鼠部分组织中的分布情况.方法 SD大鼠口服给药四聚乙醛 (40 mg/kg, 140 mg/kg, 280 mg/kg) 1周后麻醉处死, 心血、肝脏、胃组织、肾脏提取后检测, 对照组大鼠以生理盐水口服给药, 各对应组织器官样品为空白对照样.采用二氯甲烷液-液萃取, 气相色谱/质谱进行定性分析、气相色谱-FID定量分析生物检材中的四聚乙醛的浓度.结果建立了生物检材中四聚乙醛的GC/MS及GC-FID分析方法.GC/MS分析方法最低检出限为0.1 ng, GC-FID分析方法在0.010.1 mg/mL范围线性良好 (R=0.9992) , 平均回收率大于75%.高、中、低剂量染毒后四聚乙醛在胃组织中的浓度最高, 而常见药物代谢的肝脏组织中浓度最低.结论四聚乙醛在亚急性中毒大鼠体的含量分布情况为:胃组织>心血>肾脏>肝脏, 该方法操作简便快速, 检测灵敏度高, 干扰小, 适用于体内四聚乙醛含量的测定.
- 四聚乙醛 /
- 生物检材 /
- 气相色谱/质谱法 /
- 气相色谱法 /
- 体内分布
Abstract: Objective To develop the method for the determination of metaldehyde in biological material by gas chromatography/mass spectrometry (GC/MS) and GC-FID and to investigate the distribution of metaldehyde in main tissues of subacutely poisoned rats.Methods SD rats were given the oral administration of metaldehyde (40mg/kg, 140 mg/kg, 280 mg/kg) and executed by hypoxia after one week. Cardiac blood, liver, stomach and kidney were taken out and the contents of metaldehyde were detected. The rats in control group were given the oral administration of physiologic saline, and their blood and tissues were taken out correspondingly to be used as blank.The blood and tissues samples were extracted by dichloromethane. The metaldehyde was qualitatively analyzed by GC/MS and quantitatively analyzed by GC-FID.Results A GC/MS and GC-FID method for the determination of metaldehyde in biological samples was established. The limit of detection of GC/MS was 0.1ng while the GC-FID method displayed a satisfactory linearity within the concentration ranges of 0.010.1 mg/mL (R=0.9992) . The average recovery was over 75%.Conclusion The order of metaldehyde content in blood and tissues is: gastric tissues>cardiac blood>kidney>liver.This method is simple, quick, accurate, replicated and suitable for the determination of metaldehyde in biological samples.
- Metaldehyde /
- Biological samples /
- Gas chromatography/mass spectrometry /
- Gas chromatography /
- Distribution

HTML全文

参考文献(15)

[1]田盛秀, 徐瑾, 杨淞.杀灭钉螺药物的研究进展[J].中国人兽共患病学报, 2011, 27 (7) :653-656.
[2]朱丹, 李红军, 刘和香, 等.密达利对湖北钉螺酶组织化学作用的观察[J].中国寄生虫学与寄生虫病杂志, 2007, 25 (3) :198-201.
[3]	[3]LGLESIAS JAVIER, CASTILLEJO JOSE.The effects of repeated applications of the molluscide metaldehyde and the biocontrol nematode Phasmarhabditis hermaphrodita on mulluscs, earthworms, nematodes, acarids and collembolans:a two-year study in north-west spain[J].Pest Management Science, 2003, 59 (11) :1217-1224.
[4]李晓娟, 彭涛, 贾然, 等.气相色谱-质谱法测定食品基质中四聚乙醛残留量[J].食品科学, 2011, 32 (16) :224-228.
[5]李翠英.几种效果好的杀螺剂[J].河南农业, 2016, (9) :26.
[6]	[6]TAKESHI SAITO, SEIJI MORRITA.Determination of metaldehyde in human serum by headspace solid-phase microextraction and gas chromatography mass spectrometry[J].Journal of Chromatography B, 2008, 875 (2) :573-576.
[7]	[7]BETH A, VALENTINE, WILSON K, et al.Arsenic and metaldehyde toxicosis in a beef herd[J].J Vet Diagn Invest, 2007, 19 (2) :212-215.
[8]	[8]BUHI KACI J, BERMAN FREDERICK W.Report of metaldehyde and iron phosphate exposures in animals and characterization of suspected iron toxicosis in dog[J].Journal of the American Veterinary Medical Association, 2013, 242 (9) :1244-1248.
[9]	[9]BATES N S, SUTTON N M.Suspected metaldehyde slug bait poisoning in dogs:a retrospective analysis of cases reported to the veterinary poisons information service[J].The Veterinary Record, 2012, 171 (13) :324.
[10]	[10]DANIEL ROGER, LEWIS DAVID.Metaldehyde poisoning in a dairy herd[J].The Veterinary Record, 2009, 165 (19) :575-576.
[11]	[11]KIYOTA KAZUYA.Case report-fatal snail bait (mealdehyde) overdose presenting aspiration pneumonia[J].Chudoku Kenkyu, 2004, 16 (4) :453-458.
[12]	[12]SHIH CHI-CHUNG, CHANG SHY-SHIN, CHAN YILING.Acute mealdehyde poisoning intaiwan[J].Veterinary and Human Toxicology, 2004, 46 (3) :140-143.
[13]	[13]ZIMMERMANN R, STERINBERG T A.Canine status epilepticus due to acute intoxication[J].Tieraerztliche Praxis.Ausgabe K:Kleintiere-Heimtiere, 2010, 38 (5) :285-294.
[14]	[14]BLEAKLEY C, FERRIE E.Self-poisoning with metaldehyde[J].EMJ Online, 2008, 25 (6) :381-382.
[15]	[15]ELISABETH L, MAUSER, BIRGIT PUSCHNER.Evaluation of the in vitro efficacy of hemodialysis, hemoperfusion, and the combined approach on the removal of metaldehyde from canine plasma[J].Journal of Veterinary Emergency and Critical Care, 2016, 26 (4) :516-523.

相关文章(20)

[1]	敖健恒, 缪磊, 王慧颖, 章天云, 王涛, 唐永行, 胡利平, 聂胜洁. 手掌接触性生物检材DNA检出率与汗孔密度的相关性研究, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230601
[2]	苏婕俪, 李碧桃, 汪云松, 李荣生, 张兴平, 白龙慧, 王扣, 文政琦. 顶空固相微萃取-气质联用分析喙尾琵甲挥发性成分, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210802
[3]	吴乐艳, 侯雯清, 孙孔春, 杨璨瑜, 舒纹, 沈报春. 反相高效液相色谱法测定神经酸片剂中神经酸含量, 昆明医科大学学报.
[4]	卢发焕, 李继印, 李树华, 赵文嵩, 王蕊, 解润芳, 杨凯润. UPLC-MS/MS法研究滇乌碱在大鼠体内组织的分布, 昆明医科大学学报.
[5]	解润芳, 佟钧, 诸晨, 徐天勇, 解继明. 水浸GC-MS法检测生物检材中的百草枯, 昆明医科大学学报.
[6]	王尚文. GC-MS同时检测生物检材中的苯巴比妥、布洛芬、尼可刹米, 昆明医科大学学报.
[7]	单可记. 右美托咪定联合氯胺酮麻醉诱导困难气道插管, 昆明医科大学学报.
[8]	李继印. 液相色谱-质谱法测定大鼠全血中3种CYP450探针药物, 昆明医科大学学报.
[9]	李继印. 液相色谱-质谱法测定云南草乌中双酯型生物碱的含量, 昆明医科大学学报.
[10]	师国强. 支气管哮喘患者炎性因子水平与气道重构关系探讨, 昆明医科大学学报.
[11]	孙鹥. Th17相关细胞因子在丙型肝炎患者血清中的变化及意义, 昆明医科大学学报.
[12]	孙虹. 高效液相色谱法与酶法检测糖化血红蛋白结果一致性的比较, 昆明医科大学学报.
[13]	韩迪. 超高效液相色谱串联质谱法检测鸡肉中5种雌激素残留, 昆明医科大学学报.
[14]	李建钢. 视可尼纤维喉镜在困难气道中的临床应用, 昆明医科大学学报.
[15]	刘海渤. 3种提取接触性检材脱落表皮细胞DNA方法的实验研究, 昆明医科大学学报.
[16]	郑巧玲. 高效液相色谱法测定万古霉素的血药浓度, 昆明医科大学学报.
[17]	. 持续气道正压通气治疗对OSAHS患者胰岛素抵抗的影响, 昆明医科大学学报.
[18]	. 汽车安全气囊上微量检材DNA检测方法的探讨, 昆明医科大学学报.
[19]	. 多种载体人体生物检材直扩方法在DNA数据库建库中的实验研究, 昆明医科大学学报.
[20]	苏新民. 高效液相色谱法测定清脑降压片中黄芩苷的含量, 昆明医科大学学报.

施引文献

期刊类型引用(2)

1.	胡振宇，解福军，孙燕滨. 经尿道前列腺电切术在中晚期前列腺癌中的应用价值. 系统医学. 2024(13): 130-133 . 百度学术
2.	徐新宇，彭煜，朱文静. 前列腺癌内分泌治疗的研究进展. 河南大学学报(医学版). 2024(06): 391-395 . 百度学术