基质金属蛋白酶1在云南汉族口腔鳞癌发病风险中的相关性
Correlative Studies between MMP1 Gene Polymorphism and the Risk of Oral Squamous Cell Carcinomas among Han Nationality in Yunnan Province
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摘要: 目的 探讨基质金属蛋白酶 (MMP) 1基因多态性与云南汉族口腔鳞癌发病风险的相关性, 为临床上口腔鳞癌易感基因的发现与筛选提供实验参考.方法 采用聚合酶链反应-限制性片段长度多态性 (PCR-RFLP) 方法, 检测口腔鳞癌患者与健康对照组个体的MMP1启动子-1607 1G/2G单核苷酸多态性 (SNP) , 获得口腔鳞癌患者的基因型, 与健康对照组进行比较.结果 MMP1基因型在口腔鳞癌病例组和健康对照组的分布:健康对照组和病例组在MMP1启动子-1607 1G/2G中1G/1G、2G/2G、1G/2G的基因型频率分别为11.11%、37.04%、51.85%和5.66%、43.40%、50.94%.MMP1SNP与口腔鳞癌发病风险比较差异无统计学意义 (P>0.05) , 分别与高、中、低分化口腔鳞癌发病风险比较差异无统计学意义 (P>0.05) .结论 MMP1启动子-1607 1G/2G SNP可能与云南汉族口腔鳞癌发病风险无相关性.Abstract: Objective To explore the correlation between MMP1 gene polymorphisms and risk of oral squamous cell carcinomas among Han nationality in Yunnan province and to provide experimental reference for discovery and screening of susceptibility genes of oral squamous carcinoma.Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was applied to detect individual MMP1 promoter-1607 1G/2G loci single nucleotide polymorphisms (SNP) in patients with oral squamous cell carcinomas and control group. The genotype of the patients with oral squamous carcinoma was obtained and compared with that in the control group. Results The genotype frequencies of MMP1 promoter-1607 1G/2G, 1G/1G, 2G/2G, 1G/2G were 11.11% and37.04%, 51.85% and 5.66%, 43.40% and 50.94% in control group and case group respectively. There was no significant difference between MMP1 SNP and the risk of oral squamous cell carcinomas (P>0.05) . No significant difference was found when MMP1 SNP was compared with high, medium and low differentiation of oral squamous cell carcinomas (P>0.05) . Conclusion There is no correlation between MMP1 promoter-1607 1G/2G loci SNP and the risk of oral squamous carcinoma among Han nationality in Yunnan province.
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Key words:
- Oral squamous cell carcinomas /
- MMP1 /
- Polymorphism
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[1] [1]BAKER E A, LEAPER D J, HAYTER J P, et al.The matrix metalloproteinase syestem in oral squamous cell carcinoma[J].Br J Oral Maxillofac Surg, 2006, 44 (6) :482-486. [2] [2]LI X, QU L, ZHANG Y, et al.Association between promoters polymorphisms of matrix metalloproteinases and risk of digestive cancers:a meta-analysis[J].Journal of cancer research and clinical oncology.-September 1, 2013, 139 (9) :1433-1447. [3]陈丽, 向丽, 周铁军, 等.口腔鳞癌中RECK和MMP-9蛋白的表达及临床意义[J].泸州医学院学报, 2014, 37 (1) :75-78. [4] [4]ZHANG C, SONG X, ZHU M, et al.Association between MMP1-1607 1G>2G polymorphism and head and neck cancer risk:a meta-analysis[J].PLo S One, 2013, 8 (2) :e56294. [5] [5]MATSUMURA S, OUE N, KITADAI Y, et al.A single nucleotide polymorphism in the MMP-1 promoter is correlated with histological differentiation of gastric cancer[J].J Cancer Res Clin Oncol, 2004, 130 (5) :259-265. [6]戴巧群, 单海琴, 任雨, 等.基质金属蛋白酶一1基因启动子区 (-1 607) 基因多态性与肾移植后环孢素诱导牙龈增生的相关性[J].中国现代医生, 2014, 52 (30) :152-155. -
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