云南巍山回族27个Y-STR基因座遗传多态性

雷强; 肖月; 张微; 翟滇; 程宝文; 曾发明

云南巍山回族27个Y-STR基因座遗传多态性

雷强¹,
肖月¹,
张微¹,
翟滇²,
程宝文^1,2,
曾发明^1,2

1. 昆明医科大学法医学院
2. 云南省公安厅刑事侦查总队

基金项目:

基金：国家自然科学基金资助项目 (81660311);

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出版历程
- 收稿日期: 2017-04-12

Genetic Polymorphism Analysis of 27 Y-STR Loci in the Hui Population from Weishan, Yunnan Province

Funds:

基金：国家自然科学基金资助项目 (81660311);

摘要

摘要: 目的调查云南巍山回族人群的27个Y-STR的遗传多态性, 并获得相关的基础遗传学数据, 为法医学鉴定提供数据支持, 促进地区Y-STR数据库的建立.方法采集云南巍山地区171名无关男性个体血液样本, 应用YfilerRPlus试剂盒对所有样本进行27个Y-STR基因座PCR复合扩增, 用ABI 3500型遗传分析仪对扩增产物进行毛细管电泳检测, 统计相关遗传学参数.结果在171名巍山回族无关男性个体中, 27个Y-STR基因座等位基因频0.005 80.720 7之间, 基因多样性 (GD) 在0.431 40.940 8之间, 共观察到169个唯一单倍型, 1个单倍型观察到2次, 单倍型多样性 (HD) 为0.999 86, 系统识别能力 (DC) 为0.994 152.结论 27个Y-STR基因座在云南巍山回族人群中具有高度多态性, 可以为法医学鉴定及Y-STR数据库的建立提供依据.
- Y-STR /
- 遗传多态性 /
- 巍山回族
Abstract: Objective To investigate the genetic polymorphisms of 27 Y-STR loci in Hui population from Weishan, Yunnan Province, and obtain the genetic basic data of Hui population, to provide data support for forensic identification, and to promote the establishment of Y-STR database. Methods Blood samples of 171 unrelated male individuals were collected.DNA was amplified by using YfilerRPlus system and genotyped by ABI3500 Genetic Analyzer. The forensic parameters were calculated.Results In the 171 unrelated male individuals, the allele frequency was 0.00580.7207, the gene diversity (GD) was 0.43140.9408. 169 different haplotypes were observed once, 1 haplotype occurred twice.The haplotype diversity (HD) was0.99986 and the discriminative capacity (DC) was 0.994152.Conclusion The 27 Y-STR loci are highly polymorphic in Hui population from Weishan, Yunnan Province, and could be provided the basis for forensic identification and the establishment of Y-STR database.
- Y-STR /
- Genetic polymorphism /
- Weishan Hui population

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