趋化因子受体CX3CR1基因T280M多态性在云南彝族人群中的分布

陈志彬; 莫艳萍; 杨钰; 陈灿昆; 杨靖宜; 万瑞雪; 范志祥; 龙莉

趋化因子受体CX3CR1基因T280M多态性在云南彝族人群中的分布

1. 昆明医科大学
2. 昆明医科大学第一附属医院老干科
3. 昆明医科大学第二附属院生殖医学中心
4. 昆明医科大学细胞生物学和医学遗传学教研室

基金项目:

基金：国家自然科学基金资助项目 (81160164); 云南省自然科学基金资助项目 (2008CD117); 昆明医科大学大学生创新性实验计划基金资助项目 (昆医大[2017]2号);

计量
- 文章访问数: 1816
- HTML全文浏览量: 624
- PDF下载量: 36
- 被引次数: 0
出版历程
- 收稿日期: 2017-05-13

The T280M Polymorphisms of Fractalkine Receptor CX3CR1 in a Yi Nationality Population of Yunnan Province

Funds:

摘要

摘要: 目的研究云南省晋宁县彝族人群CX3CR1基因T280M多态性分布的情况, 为探讨CX3CR1基因相关性疾病的发病机制奠定基础.方法采用PCR-RFLP技术对居住在云南省晋宁县268例彝族健康个体的CX3CR1基因T280M多态性进行分析, 计算MM、MT、TT基因型频率和M、T等位基因频率, 与其他人群该位点多态性进行比较.结果云南省晋宁县彝族CX3CR1基因的MM、MT、TT基因型频率分别为1.12%、11.94%和86.94%, M和T等位基因频率分别为7.09%和92.91%.与汉族、维吾尔族、希腊、法国、塞尔维亚、印度、意大利和高加索人群比较, 无论是基因型分布还是基因频率差异有统计学意义 (P<0.05) ;但与景颇族比较中差异无统计学意义 (P>0.05) .结论 CX3CR1基因T280M多态性分布存在民族、种族差异, 且种族间的差异大于民族间的差异.
- CX3CR1基因 /
- 基因多态性 /
- 彝族 /
- 分布
Abstract: Objective To investigate the distribution of the CX3CR1 gene T280 M polymorphism in a Yi nationality population of Yunnan province, and lay the foundation of exploring the pathogenesis of CX3CR1 gene ralated diseases. Methods We studied 268 healthy individuals of Yi nationality in a rural area of Jinning, Yunnan province.Genotype determination of the CX3CR1 gene T280 M polymorphism was performed using Polymerase Chain Reaction-Restriction fragment Length Polymorphism ( PCR-RFLP) technique.The frequencies of MM, MT, TT genotypes and M, T alleles were calculated. We conducted some analysis by comparing with results of other populations collected from previous studies.Results In the healthy Yi nationality population of Yunnan province, CX3CR1 genotype frequencies for the MM, MT and TT genotypes were1.12%, 11.94% and 86.94%. Deriving from those, the allele frequencies for the M and T allele were 7.09%and 92.91% respectively.Significant differences in the distribution of CX3CR1 genotypes and the allele frequencies were observed between the Yunnan Yi people and other nationality populations (The han, The uighurs, Greece, France, Serbia, India, Italy and The Caucasus) (P <0.05) ; whereas there was no significant difference with the Jingpo nationality (P>0.05) .Conclusions Differences of the genotype or allele frequency of the CX3CR1 T280 M polymorphism exist in different nationalities and different races, and the national difference is more obvious than ethnic ones.
- CX3CR1 gene /
- Genetic polymorphism /
- Yi nationality /
- Distribution

HTML全文

参考文献(27)

[1]	[1]IMAI T, HIESHIMA K, HASKELL C, et al.Identification and molecular charact-erization of fractalkine receptor CX3CR1, which mediates both leukocyte mi-gration and adhesion[J].Bioscience Biotechnology&Biochemistry, 1997, 91 (4) :521-530.
[2]	[2]KOBAYASHI T, OKAMOTO S, IWAKAMI Y, et al.Exclusive increase of CX3C-R1+CD28-CD4+T cells in inflammatory bowel disease and their recruitment as intraepithelial lymphocytes[J].Inflammatory Bowel Diseases, 2007, 13 (7) :837-846.
[3]	[3]SORIANO S G, AMARAVADI L S, WANG Y F, et al.Mice deficient in fractalkine are less susceptible to cerebral ischemia-reperfusion injury[J].Journal of N-euroimmunology, 2002, 125 (1-2) :59-65.
[4]	[4]OWLASIUK P, ZAJKOWSKA J M, PIETRUCZUK M, et al.Fractalkine structure, func-tions and biological activity[J].Polski Merkuriusz Lekarski Organ Polskiego Towarzystwa Lekarskiego, 2009, 26 (153) :253-257.
[5]	[5]KOZIOLEK M J, MLLER G A, ZAPF A, et al.Role of CX3C-chemokine CX3-CL/fractalkine expression in a model of slowly progressive renal failure[J].Nephrology Dialysis Transplantation, 2010, 25 (3) :684-698.
[6]	[6]MC DERMOTT D H, COLLA J S, KLEEBERGER C A, et al.Genetic polymorphism in CX3CR1 and risk of HIV disease[J].Science, 2000, 290 (5499) :2031.
[7]	[7]MAHO A, BENSIMON A, VASSART G, et al.Mapping of the CCXCR1, CX3C-R1, CCBP2 and CCR9 genes to the CCR cluster within the 3p21.3 region of the human genome[J].Cytogenetics&Cell Genetics, 2000, 87 (3-4) :265-268.
[8]甄瑞峰, 唐灵.FKN与疾病的研究进展[J].医学综述, 2009, 15 (20) :3061-3064.
[9]	[9]ZHENDONG L I, SHIJIAN LUO, FENG WANG, et al.An association study betwe-en polymorphisms of the fractalkine receptor gene, CX3CR1, and cerebral in-farction in the Han Chinese population[J].Journal of the Neurological Sciences, 2012, 320 (1-2) :12-15.
[10]刘明旭, 王福生, 洪卫国, 等.中国汉族和维吾尔族艾滋病病毒感染者与未感染人群中CX3CR1基因多态性的分布[J].中华流行病学杂志, 2003, 24 (7) :595-598.
[11]	[11]APOSTOLAKIS S, BARITAKI S, KOCHIADAKIS G E, et al.Effects of polymorphi-sms in chemokine ligands and receptors on susceptibility to co-ronary artery disease[J].Thrombosis Research, 2007, 119 (1) :63-71.
[12]	[12]ZERBIB J, PUCHE N, RICHARD F, et al.No association between the T280M p-olymorphism of the CX3CR1 gene and exudative AMD[J].Experimental Eye Research, 2011, 93 (4) :382-386.
[13]	[13]STOJKOVI L, DJURI T, STANKOVI A, et al.The association of V249I and T280M fractalkine receptor haplotypes with disease course of multiple sclero-sis[J].Journal of Neuroimmunology, 2012, 245 (1-2) :87-92.
[14]	[14]SINGH N, SINHA N, KUMAR S, et al.Suraks-ha Agrawal Polymorphism in c-hemokine receptor genes and risk of acute myocardial infarction in North In-dian population[J].Molecular Biology Reports, 2012, 39 (3) :2753-2759.
[15]	[15]NORATA G D, GARLASCHELLI K, ONGARI M, et al.Effects of fractalkine recept-or variants on common carotid artery intima-media thickness[J].Stroke, 2006, 37 (6) :1558-1561.
[16]	[16]FAURE S, MEYER L, COSTAGLIOLA D, et al.Rapid progression to AIDS in HIV+individuals with a structural variant of the chemokine receptor CX3CR1[J].Science, 2000, 287 (5461) :2247-2277.
[17]叶峻杰, 王福生, 彭林, 等.云南傣族景颇族人群中与艾滋病相关的CX3CR1基因多态性分布[J].中国优生与遗传杂志, 2004, 12 (3) :16-17.
[18]	[18]WONG BWC, WONG D, MCMANUS BM.Characterization of fractalkine (C-X3CL1) and CX3CR1 in human coronary arteries with native atherosclerosis, diabetes mellitus, and transplant vascular disease[J].Cardiovascular Pathology, 2002, 11 (6) :332-338.
[19]	[19]YADAV A K, KUMAR V, JHA V.Association of chemokine receptor CX3C-R1 V249I and T280M polymorphisms with chronic kidney disease[J].Indian Journal of Nephrology, 2016, 26 (4) :275-279.
[20]	[20]BABAKURBAN S T, ERBEK S S, TERZI Y K, et al.Fractalkine receptor polymo-rphism and chronic to-nsillitis[J].European Archives of Oto-Rhino-Laryngology, 2014, 271 (7) :2045-2048.
[21]彭旭, 张智博, 唐璐, 等.缺血性脑血管病患者趋化因子受体CX3C-R1基因T280M多态性的研究[J].临床神经病学杂志, 2008, 21 (5) :329-332.
[22]	[22]MCDERMOTT D H, HALCOX J P, SCHENKE W H, et al.Association between po-lymorphism in the chemokine receptor CX3CR1 and coronary vascular endo-thelial dysfunction and atherosclerosis[J].Circulation Research, 2001, 89 (5) :401-407.
[23]龙莉, 杨榆玲, 瞿秋, 等.血管紧张素原基因M235T多态性在云南彝族人群中的分布[J].昆明医科大学学报, 2012, 33 (11) :4-7.
[24]龙莉, 莫艳萍, 何永蜀, 等.血管紧张素转换酶基因I/D多态性在云南省彝族人群中的分布[J].昆明医科大学学报, 2009, 30 (7) :46-49.
[25]杨榆玲, 龙莉, 瞿秋, 等.血管紧张素Ⅱ1型受体A1166C多态性在云南彝族人群中的分布[J].昆明医科大学学报, 2012, 33 (5) :43-46.
[26]陈国文, 尤开, 龙莉, 等.MCP-1G-2518A多态性在云南彝族人群中的分布[J].昆明医科大学学报, 2016, 37 (2) :5-9.
[27] 尤开, 陈国文, 刘珊灵, 等.CCR2b基因190G/A多态性在云南省彝族人群中的分布[J].昆明医科大学报, 2016, 37 (1B) :565-568.

相关文章(20)

[1]	马承娅, 杨江, 陈曦, 徐茜, 尹丕轩, 舒芯, 彭红瑜, 范志祥, 龙莉. 髓过氧化物酶-463G/A基因多态性与彝族原发性高血压的相关性, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20240408
[2]	谭洪, 殷和佳, 李妍, 胡琴, 李斓, 任遵丹, 石柔. DDAH1基因836A/T多态性与云南地区汉族2型糖尿病肾脏疾病的相关性分析, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230621
[3]	陆小华, 袁洪新. BTLA、CTLA-4基因多态性与肝癌TACE联合靶向治疗疗效及预后相关性, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20230927
[4]	陈新元, 吴晖, 莫亚雄, 韩雁冰, 陈新利, 朱文叶, 黄蕊, 唐薇. NRG1-ErbB4基因多态性与局灶性癫痫易感性的关系, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210607
[5]	彭红瑜, 范志祥, 张延洁, 舒芯, 张馥麟, 龙莉. CYP11B2基因-344C/T多态与云南双河乡彝族人群原发性高血压的相关性, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210702
[6]	皮婷, 梁月琴, 丁莉, 杨玲, 向盈盈. 妊娠期女性MTHFR 677C > T基因多态性分布特征及其与妊娠高血压疾病的相关性, 昆明医科大学学报. doi: 10.12259/j.issn.2095-610X.S20210215
[7]	王乐, 范志祥, 陈志彬, 彭红瑜, 张延洁, 张璐, 万瑞雪, 龙莉. t-PA基因Alu重复序列I/D多态性在云南彝族人群中的分布, 昆明医科大学学报.
[8]	欧阳培刚, 张桂敏, 刘寅强. NAT2基因多态性与云南汉族华法林剂量的相关性, 昆明医科大学学报.
[9]	吴騻, 何利平, 李晓梅, 白嫒莲, 季云瑞, 孟金良. 云南省彝族居民健康的公平性, 昆明医科大学学报.
[10]	朱柯颖, 蒋涛, 盘婕, 范志祥, 张璐, 张延洁, 吴艳瑞, 龙莉. CYP4A11基因rs9333025 A/G多态性在云南彝族人群中的分布, 昆明医科大学学报.
[11]	陈灿昆, 陈志彬, 杨靖宜, 杨钰, 范志祥, 张延洁, 张璐, 龙莉. CBS基因844ins68多态性在云南彝族中的分布, 昆明医科大学学报.
[12]	黄红. 云南汉族原发性高血压ACE基因多态性与厄贝沙坦降压疗效相关性, 昆明医科大学学报.
[13]	陈国文. MCP-1 G-2518A多态性在云南彝族人群中的分布, 昆明医科大学学报.
[14]	王祝君. β-烯醇化酶基因多态性与COPD骨骼肌萎缩的相关性, 昆明医科大学学报.
[15]	詹辉. CD3+T淋巴细胞在膀胱癌肿瘤上皮与癌旁尿路上皮中的分布差异研究, 昆明医科大学学报.
[16]	刘玲. 云南省重症新生儿黄疸与Gly71Arg基因多态性研究, 昆明医科大学学报.
[17]	孟丽红. Nogo及雌激素受体基因多态性与多发性硬化的研究, 昆明医科大学学报.
[18]	杨榆玲. 血管紧张素II 1型受体A1166C多态性在云南彝族人群中的分布, 昆明医科大学学报.
[19]	龙莉. 血管紧张素原基因M235T多态性在云南彝族人群中的分布, 昆明医科大学学报.
[20]	文勇. 彝族新生儿先天性皮肤缺损1例诊治体会, 昆明医科大学学报.