维生素D受体基因ApaⅠ、BsmⅠ位点单核苷酸多态性与2型糖尿病肾病的相关性

向茜; 李万碧; 张弦; 刘华; 王玉明; 杨才; 白云霞

维生素D受体基因ApaⅠ、BsmⅠ位点单核苷酸多态性与2型糖尿病肾病的相关性

1. 昆明医科大学第五附属医院内分泌科
2. 昆明医科大学第一附属医院检验科
3. 昆明医科大学第二附属医院检验科

基金项目:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2012FB085); 云南省教育厅科学研究基金资助项目 (2012C020);

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出版历程
- 收稿日期: 2017-05-22

Correlation between Vitamin D Receptor (VDR) Gene ApaⅠ, BsmⅠ Single Nucleotide Polymorphism and Diabetic Kidney Disease in Type 2 Diabetic Patients

Funds:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2012FB085); 云南省教育厅科学研究基金资助项目 (2012C020);

摘要

摘要: 目的探讨VDR基因多态性与2型糖尿病肾病的相关性.方法应用TaqMan荧光探针技术, 对235例2型糖尿病患者其中正常白蛋白尿 (DKD0) 组82例, 微量白蛋白组 (DKD1) 组66例, 大量白蛋白尿组 (DKD2) 组87例和86例健康对照者 (NGT) 组的VDR基因BsmⅠ、ApaⅠ位点SNP进行检测, 比较和分析各组间基因型频率和等位基因频率以及相关临床资料.结果 (1) BsmⅠ位点GG、GA、AA基因型频率分别为0.931、0.066、0.003, 等位基因G和A频率分别为0.964、0.036;ApaⅠ位点CC、CA、AA因型频率分别0.536、0.371、0.093, 等位基因C和A频率分别为0.721、0.279; (2) DKD2组ApaⅠ位点C等位基因频率 (0.770) 高于DKD0组患者 (0.671) , A等位基因频率 (0.230) 低于于DKD0组患者 (0.329) , P<0.05; (3) Logistic回归分析表明VDR基因BsmⅠ位点GG基因型可能是T2DKD发生的独立保护因素 (OR=0.159, P<0.05) , 但与DKD进展无关;ApaⅠ位点SNP与T2DKD的发生及进展均无关 (P>0.05) .25 (OH) D缺乏或不足是T2DKD进展的危险因素 (OR=1.957, P<0.05) .结论 VDR基因BsmⅠ位点GG基因型可能是T2DKD发生的独立保护因素, 但与DKD进展无关;ApaⅠ位点SNP与T2DKD的发生及进展均无关.
- 维生素D受体基因 /
- 单核苷酸多态性 /
- 2型糖尿病 /
- 糖尿病肾病 /
- 25羟维生素D
Abstract: Objetive To investgate the association of Vitamin D receptor gene ApaⅠ, BsmⅠ single nucleotide polymorphism with diabetic kidney disease in type 2 diabetic patients.Methods The single nucleotide polymorphism of VDR gene in 235 type 2 diabetes [including 82 normal albuminuria (DKD0 group) , 66microalbuminuria (DKDl group) and 87 macroalbuminuria (DKD2 group) ]and 86 normal controls were detected by Taq Man fluorescent probe. The genotype frequency and allele frequency and relative clinical datawere compared among groups. Results (1) BsmⅠ GG, GA, AA genotype frequencies were 0.931, 0.066, 0.003, allele G and allele G frequencies were 0.964 and 0.036. ApaⅠ CC、CA、AA genotype frequencies were0.536, 0.371, 0.093, allele C and allele A frequencies were 0.721 and 0.279. (2) ApaⅠ allele C frequencies (P<0.05) in DKD2 group (0.770) were significantly higher than in DKD0 group (0.671) , and the allele A frequencies (P<0.05) in DKD2 group (0.230) were significantly lower than in DKD0 group (0.329) (P<0.05) . (3) Logistic analysis showed that BsmⅠ GG genotype (OR = 0.159, P<0.05) was the independent protective factors of DKD occurrence, but it was not related to T2 DKD development. ApaⅠ single nucleotide polymorphism was not related to T2 DKD occurrence and development ( P >0.05) . 25 ( OH) D deficiency/insufficiency may b e the independent risk factor of T2 DKD development (OR = 1.957, P <0.05) .Conclusions BsmⅠ GG genotype is an independent protective factor of T2 DKDoccurrence, but it is not related to T2 DKD development.ApaⅠ singlenucleotide polymorphism is not related to T2 DKD occurrence and development.
- Vitamin D receptor gene /
- Single nucleotide polymorphism /
- Type 2 diabetes mellitus /
- Diabetic kidney disease /
- 25-hydroxy-vitamin D

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参考文献(32)

[1]	[1]DANESCU L G, LEVY S, LEVY J.Vitamin D and diabetes mellitus[J].Endocrine, 2009, 35 (1) :7-11.
[2]邢丹, 马信龙, 马剑雄, 等.维生素D受体基因BsmⅠ、AoaⅠ、TaqⅠ、FokⅠ和Cdx-2位点多态性与骨质疏松性椎体骨折相关性的Meta分析[J].中华骨质疏松和骨矿盐疾病杂志, 2013, 6 (2) :165-173.
[3]	[3]American Diabetes Association.Standards of Medical Care in Diabetes-2010[J].Diabetes Care, 2010, 33 (Suppl1) :11-61.
[4]中华医学会内分泌学分会.中国成人糖尿病肾脏病临床诊断的专家共识[J].中华内分泌代谢杂志, 2015, 31 (5) :379-385.
[5]	[5]RENZAHO A M, NOWSON C, KAUR A, et al.Prevalence of vitamin D insufficiency and risk factors for type 2 diabetes and cardiovascular disease among African migrant and refugee adults in Melbourne:A pilot study[J].Asia Pac J Clin Nutr, 2011, 20 (3) :397-403.
[6]	[6]AGARWAL R, VITAMIN D.Pproteinuria, diabetic nephropathy, and progression of CKD[J].Clin J Am Soc Nephrol, 2009, 4 (9) :1523-1528.
[7]	[7]TALAEI A, MOHAMADI M, ADGI Z.The effect of vitamin D on insulin resistance in patients with type 2 diabetes[J].Diabetol Metab Syndr, 2013, 5 (1) :8.
[8]王亚娟, 任建民, 肖晓燕, 等.维生素D与糖尿病肾病相关性研究进展[J].中国实用内科杂志, 2014, 34 (7) :722-724.
[9]	[9]YANG C Y, LEUNG P S, ADAMOPOULOS I E, et al.The implication of vitamin D and autoimmunity:A comprehensive review[J].Clin Rev Allergy Immunol, 2013, 45 (2) :217-226.
[10]	[10]HUGHES D J, MCMANUS R, NEARY P, et al.Common variation in the vitamin D receptor gene and risk of inflammatory bowel disease in an Irish case-control study[J].Eur J Gastroenterol Hepatol, 2011, 23 (9) :807-812.
[11]	[11]WANG S, WANG X, WU J, et al.Association of vitamin D receptor gene polymorphism and calcium urolithiasis in the Chinese Han population[J].Urol Res, 2012, 40 (4) :277-284.
[12]	[12]BID H K, KONWAR R, AGGARWAL C G, et al.Vitamin D receptor (Fok I, Bsm I and Taq I) gene polymorphisms and type 2 diabetes mellitus:A North Indian study[J].Indian J Med Sci, 2009, 63 (5) :187-194.
[13]	[13]UKAJI M1, SAITO Y, FUKUSHIMA-UESAKA H, et al.Genetic variations of VDR/NR1I1 encoding vitamin D receptor in a Japanese population[J].Drug Metab Pharmacokinet, 2007, 22 (6) :462-467.
[14]张鹏, 苏文凌, 沈保爽, 等.天津地区汉族人2型糖尿病与维生素D受体基因多态性的研究[J].天津医药, 2008, 36 (4) :255-257.
[15]石艳军, 沈轶, 蔡立清, 等.维生素D受体基因多态性与糖尿病的相关性[J].中国糖尿病杂志, 2007, 15 (4) :219-221.
[16]王瑞英, 王宏, 周璐, 等.维生素D受体基因 (VDR) 多态性与儿童铅中毒易感性的研究[J].中国妇幼保健, 2012, 27 (21) :3312-3314.
[17]祁娟, 许烨.妊娠糖尿病患者的维生素D受体基因单核苷酸多态性研究[J].检验医学与临床, 2013, 10 (20) :2663-2665.
[18]朱钊, 韩睿.维生素D及其受体基因与糖尿病视网膜病变的关系[J].中华糖尿病杂志, 2011, 3 (6) :493-496.
[19]	[19]REMES T, VISNEN S B, MAHONEN A, et al.Bone mineral density, body height, and vitamin D receptor gene polymorphism in middle-aged men[J].Ann Med, 2005, 37 (5) :383-392.
[20]	[20]JOHN E M, SCHWARTZ G G, KOO J, et al.Sun exposure, vitamin D receptor gene polymorphisms, and risk of advanced prostate cancer[J].Cancer Res, 2005, 65 (12) :5470-5479.
[21]夏盛隆, 夏宣平, 王文星, 等.维生素D受体基因多态性及血清25-羟维生素D水平与溃疡性结肠炎的关系[J].中华医学杂志, 2014, 94 (14) :1060-1066.
[22]	[22]LIN W Y, WAN L, TSAI C H, et al.Vitamin D receptor gene polymorphisms are associated with risk of Hashimoto's thyroiditis in Chinese patients in Taiwan[J].J Clin Lab Anal, 2006, 20 (3) :109-112.
[23]任晓峰, 王佳, 颜小銮, 等.维生素D受体基因VDR与胃癌易感性关系[J].中国公共卫生, 2014, 30 (7) :948-951.
[24]韩睿, 董霞, 苏炳驰, 等.维生素D受体TaqⅠ、ApaⅠ基因多态性与昆明汉族2型糖尿病肾病的相关性研究[J].中国医药导报, 2015, 12 (9) :69-72.
[25]易斌, 张浩, 赵延, 等.维生素D受体基因Bsm I多态性与2型糖尿病肾病的关系[J].中华肾脏病杂志, 2012, 28 (4) :281-285.
[26]侯清涛, 李舍予, 吕霞飞, 等.维生素D受体基因多态性与2型糖尿病易患性的Meta分析[J].中国全科医学, 2014, 17 (27) :3225-3231.
[27]	[27]DE SOUZA C M, BRAOSI A P, LUCZYSZYN S M, et al.Association between vitamin D receptor gene polymorphisms and susceptibility to chronic kidney disease and periodontitis[J].Blood Purif, 2007, 25 (5-6) :411-419.
[28]卓小群, 刘素筠, 闫萌萌, 等.维生素D受体基因多态性与2型糖尿病肾病及血清25-羟基维生素D3的关系[J].中国药物与临床, 2015, 15 (3) :387-389.
[29]	[29]NOSRATABADI R, ARABABADI M K, SALEHABAD VA, et al.Polymorphisms within exon 9 but not intron 8of the vitamin D receptor are associated with the nephropathic complication of type-2 diabetes[J].Int J Immunogenet, 2010, 37 (6) :493-497.
[30]	[30]HOLICK M F.Vitamin D deficiency[J].N Engl J Med, 2007, 357 (3) :266-281.
[31]	[31]UITTERLINDEN A G, FANG Y, VAN MEURS J B, et al.Genetics and biology of vitamin D receptor polymorphisms[J].Gene, 2004, 338 (2) :143-156.
[32]	[32]VALDIVIELSO J M, FERNANDEZ E.Vitamin D receptor polymorphisms and diseases.[J].Clin Chim Acta, 2006, 371 (1-2) :1-12.

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