儿童肺炎支原体肺炎与呼出气一氧化氮的相关性

孙宇星; 谭力; 郝芮; 李明; 甘政艳; 吴澄清

儿童肺炎支原体肺炎与呼出气一氧化氮的相关性

昆明市儿童医院呼吸内科

基金项目:

基金：昆明市西山区科技计划基金资助项目 (西科字23号);

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出版历程
- 收稿日期: 2017-04-12

The Correlation between Exhaled Nitric Oxide and Mycoplasma Pneumoniae Pneumonia in Children

Funds:

基金：昆明市西山区科技计划基金资助项目 (西科字23号);

摘要

摘要: 目的研究肺炎支原体肺炎 (MPP) 患儿呼出气一氧化氮 (FeNO) 水平及其与支气管肺泡灌洗液 (BALF) 菌量高低的相关性.方法选择2016年1月至2016年11月于昆明医科大学附属儿童医院住院的42例MPP患儿, 选取50例健康同龄儿童作对照组, 对比2组儿童FeNO测定值;采用实时荧光定量PCR (RT-PCR) 法检测MPP患儿BALF中肺炎支原体的DNA定量 (MP-DNA) , 将其分为低菌量组 (MP-DNA定量≤106copies/m L) 及高菌量组 (MP-DNA定量>106 copies/m L) , 对比不同菌量组的FeNO测值;同时, 根据临床将MPP患儿分为普通MPP组及难治性肺炎支原体肺炎 (RMPP) 组, 对其FeNO测值进行对比.结果 MPP组FeNO水平[ (27.48±5.34) bbp]显著高于健康对照组[ (14.24±4.21) bbp], 差异有统计学意义 (P<0.05) ;高菌量组FeNO水平[ (27.33±6.20) bbp]与低菌量组[ (27.58±4.74) bbp]相当, 差异无统计学意义 (P>0.05) ;RMPP组FeNO水平[ (29.88±4.51) bbp]高于普通MPP组[ (26.00±5.35) bbp].结论肺炎支原体感染可引起FeNO水平增高, 但其与所感染肺炎支原体菌量高低之间不存在有相关性;与普通MPP相比, RMPP对患儿小气道的损伤可能更突出.
- 肺炎支原体肺炎 /
- 肺泡灌洗液 /
- 呼出气一氧化氮
Abstract: Objective To observe the correlation between the exhaled nitric oxide (FeNO) and mycoplasma load in bronchialveolar lavage fluid (BALF) in children with Mycoplasma pneumoniae pneumonia (MPP) .Methods 42 inpatients in affiliated children's hospital of kunming medical university were enrolled from January to November in 2016, and 50 healthy children were selected as control group.Then the value of FeNO were compared between two groups.MP DNA in BALF was detected quantitatively by fluorescent real-time PCR.Children with MPP were divided into two groups of low MP-DNA load group and high MP-DNA load group according to the copies of MP DNA in BALF less than or equal to106 copies/m L or not.The value of FeNO were compared between groups with different copies of MP DNA.We also compared the level of FeNO between normal MPP group and refractory Mycoplasma pneumonia pneumonia (RMPP) group.Results The level of FeNO from the patients with MPP[ (27.48±5.34) bbp]was significantly higher than that of control group[ (14.24±4.21) bbp].The level of FeNO between the low MP-DNA load group[ (27.58±4.74) bbp] and the high MP-DNA load group[ (27.33±6.20) bbp]had no statistical significance.The level of FeNO from the patients with RMPP[ (29.88±4.51) bbp]was higher than that of normal MPP group[ (26.00±5.35) bbp].Conclusion MP infection increase the production of FeNO, andit demonstrates that there is no relationship between the value of FeNO with the MP-DNA load.Compared with normal MPP, the damage to the small airway maybe more prominent in RMPP.
- Mycoplasma pneumoniae pneumonia /
- Bronchialveolar lavage fluid /
- Exhaled nitric oxide

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参考文献(14)

[1]	[1]HEJ, LIUMH, YEZF, et al.Insights into the pathogenesis of Mycoplasma pneumoniae[J].Mol Med Rep, 2016, 14 (5) :4030-4036.[1]HEJ, LIUMH, YEZF, et al.Insights into the pathogenesis of Mycoplasma pneumoniae[J].Mol Med Rep, 2016, 14 (5) :4030-4036.
[2]	[2]YAO T C, TSAI HJ, TU Y L, et al.Multiplexed immunoglobulin E sensitization in relation to exhaled nitric oxide in a population sample of children[J].Allergy, 2014, 69 (5) :678-682.[2]YAO T C, TSAI HJ, TU Y L, et al.Multiplexed immunoglobulin E sensitization in relation to exhaled nitric oxide in a population sample of children[J].Allergy, 2014, 69 (5) :678-682.
[3]胡亚美, 江载芳.诸福棠实用儿科学[M].第8版.北京:人民卫生出版社, 2015:1253.[3]胡亚美, 江载芳.诸福棠实用儿科学[M].第8版.北京:人民卫生出版社, 2015:1253.
[4] 中华医学会儿科学分会呼吸学组《中华实用儿科临床杂志》编辑委员会.儿童肺炎支原体肺炎诊治专家共识 (2015年版) [J].中华实用儿科临床杂志, 2015, 30 (17) :1304-1308.[4]中华医学会儿科学分会呼吸学组《中华实用儿科临床杂志》编辑委员会.儿童肺炎支原体肺炎诊治专家共识 (2015年版) [J].中华实用儿科临床杂志, 2015, 30 (17) :1304-1308.
[5]	[5]MIDULLA F, DEBLIC J, BARBATO A, et al.Flexible endoscopy of paediatric airways[J].Eur Respir J, 2003, 22 (4) :698-708.[5]MIDULLA F, DEBLIC J, BARBATO A, et al.Flexible endoscopy of paediatric airways[J].Eur Respir J, 2003, 22 (4) :698-708.
[6]	[6] AMERICA THORACIC SOCIETY, EUROPEAN RESPIRATORY SOCIETY.ATS/ERS recommendations for standardized procedures for theonline and off line measurement of exhaled lower respiratory nitric oxide and nasal nitric oxide[J].Am J Respir Crit Care Med, 2005, 171 (8) :912-930.[6]AMERICA THORACIC SOCIETY, EUROPEAN RESPIRATORY SOCIETY.ATS/ERS recommendations for standardized procedures for theonline and off line measurement of exhaled lower respiratory nitric oxide and nasal nitric oxide[J].Am J Respir Crit Care Med, 2005, 171 (8) :912-930.
[7]	[7]SEKINEH, TAGUCHIH, WATANABEH, et al.Immunologicalanalysisandpathologicalexamination of gnotobioticmicemonoassociatedwith Mycoplasmapneumoniae[J].J Med Microbiol, 2009, 58 (Pt6) :697-705.[7]SEKINEH, TAGUCHIH, WATANABEH, et al.Immunologicalanalysisandpathologicalexamination of gnotobioticmicemonoassociatedwith Mycoplasmapneumoniae[J].J Med Microbiol, 2009, 58 (Pt6) :697-705.
[8]	[8]BOSE S, SEGOVIAJA, SOMARAJANSR, et al.ADPribosylation Of NLRP3 by Mycoplasma pneumoniae CARDS toxin regulatesinflammasome activity[J].MBio, 2014, 5 (6) :e02186.[8]BOSE S, SEGOVIAJA, SOMARAJANSR, et al.ADPribosylation Of NLRP3 by Mycoplasma pneumoniae CARDS toxin regulatesinflammasome activity[J].MBio, 2014, 5 (6) :e02186.
[9]	[9]HARDY R D, COALSON J J, PETERS J, et al.Analysis of pulmonary inflammation and function in the mouse and baboon after exposure to mycoplasma pneumoniae CARDS toxin[J].PLo S One, 2009, 4 (10) :e7562.[9]HARDY R D, COALSON J J, PETERS J, et al.Analysis of pulmonary inflammation and function in the mouse and baboon after exposure to mycoplasma pneumoniae CARDS toxin[J].PLo S One, 2009, 4 (10) :e7562.
[10]陈广道, 陈爱欢.儿童肺炎支原体感染相关喘息的研究进展[J].中华儿科杂志, 2012, 50 (10) :756-759.[10]陈广道, 陈爱欢.儿童肺炎支原体感染相关喘息的研究进展[J].中华儿科杂志, 2012, 50 (10) :756-759.
[11]	[11]SCHLEICHFN, SEIDELL, SELEJ, et al.Exhalednitricoxidethresholdsassociatedwithasputumeosinophilcount≥3%inacohortofunselectedpatientswithasthma[J].Thorax, 2010, 65 (12) :1039-1044.[11]SCHLEICHFN, SEIDELL, SELEJ, et al.Exhalednitricoxidethresholdsassociatedwithasputumeosinophilcount≥3%inacohortofunselectedpatientswithasthma[J].Thorax, 2010, 65 (12) :1039-1044.
[12]	[12]REDDELHK, TAYLORDR, BATEMANED, et al.Anofficial american thoracic society european respiratory society statement:asthma control and exacerbations:standardizing end points for clinical asthma trials andc linical practice[J].Am J Respir Crit Care Med, 2009, 180 (1) :59-99.[12]REDDELHK, TAYLORDR, BATEMANED, et al.Anofficial american thoracic society european respiratory society statement:asthma control and exacerbations:standardizing end points for clinical asthma trials andc linical practice[J].Am J Respir Crit Care Med, 2009, 180 (1) :59-99.
[13]黄凤, 季伟, 严永东, 等.部分呼出气一氧化氮检测在肺炎支原体肺炎中的意义[J].中华实用儿科临床杂志, 2015, 30 (4) :258-260.[13]黄凤, 季伟, 严永东, 等.部分呼出气一氧化氮检测在肺炎支原体肺炎中的意义[J].中华实用儿科临床杂志, 2015, 30 (4) :258-260.
[14]孙慧明, 季伟, 蔡利红, 等.不同年龄肺炎支原体肺炎患儿胸片与实验室特点比较[J].临床儿科杂志, 2012, 30 (4) :342-346.[14]孙慧明, 季伟, 蔡利红, 等.不同年龄肺炎支原体肺炎患儿胸片与实验室特点比较[J].临床儿科杂志, 2012, 30 (4) :342-346.

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