2型糖尿病膀胱大鼠模型的制作及尿流动力学变化

马俭飞; 王淞; 苏婷; 唐艳; 张钰泉; 方克伟

2型糖尿病膀胱大鼠模型的制作及尿流动力学变化

1. 昆明医科大学
2. 昆明医科大学第二附属医院

基金项目:

基金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目 (2015FB086); 云南省教育厅科学研究基金资助项目 (2015C005Z); 昆明医科大学校级大学生创新性研究基金资助项目 (CX201581);

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出版历程
- 收稿日期: 2017-06-05

Preparation of Rat Model of Diabetic Cystopathy (DCP) and the Changes of Urodynamics of Rats with DCP

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摘要

摘要: 目的探讨2型糖尿病膀胱大鼠模型的制作方法及其尿流动力学变化情况.方法将40只雌性大鼠体 (200250) g分为2组:A组为对照组 (22只) , B组为实验组 (18只) .实验组经高糖高脂喂养四周后腹腔内注射链脲佐菌素 (STZ) (30 mg/kg一次性腹腔注射) 诱导建立大鼠2型糖尿病膀胱模型;对照组常规饲养4周后腹腔注射相同剂量枸橼酸缓冲液.2组均采用尾静脉测定大鼠血糖, 每周1次, 连续4周.12周时2组大鼠进行膀胱容量、膀胱压及漏尿点压测定, 连续记录3次.结果与对照组相比较, 实验组血糖明显升高, STZ建模成功率100%;尿动力学结果显示实验组充盈期膀胱容量明显高于对照组, 膀胱压和漏尿点明显低于对照组.结论 STZ用于建立糖尿病大鼠膀胱模型成功率高, 其膀胱尿道功能发生显著改变.
- 糖尿病膀胱 /
- 链脲佐菌素 /
- 2型糖尿病 /
- 尿流动力学
Abstract: Objective To explore the modeling of diabetic cystopathy (DCP) and the changes of urodynamics in rats with DCP.Methods 40 female rats (200-250 g) were divided into two groups: group A, the controlled group (22 rats) and group B (18 rats) , the experimental group.The model of DCP (experimental group) was established with the intraperitoneal injection of streptozotocin (STZ) after 4 weeks feeding of high-sugar high-fat.Group A was injected citrate buffer intraperitoneally (the same dose of STZ) after 4 weeks normal feeding.Both groups were tested the blood sugar through the tail vein, 1 time a week, four times altogether.Twelve weeks later, the bladder capacity, bladder pressure and leakage point pressure were tested with urodynamic instument.The results were tested 3 times.Results The blood sugar of group B was higher obviously than group A.And the successful modeling rate of DCP with STZ was 100%.Compared wth group A, the filling bladder capacity was higher significantly, the bladder pressure and leakage point pressure were lower significantly in group B.Conclusion The successful modeling rate of DCP with STZ is high and the urethra and bladder function changes are significant in DCP rats.
- Diabetic cystopathy /
- Streptozotocin /
- Diabetes millitus 2 /
- Urodynamics

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参考文献(20)

[1]	[1]DANESHGARI F, LIU G, BIRDER L, et al.Diabetic bladder dysfunction;Current translational knowledge[J].J Urol, 2009, 182 (6 Suppl) :S18-26.[1]DANESHGARI F, LIU G, BIRDER L, et al.Diabetic bladder dysfunction;Current translational knowledge[J].J Urol, 2009, 182 (6 Suppl) :S18-26.
[2]	[2]BROWN J S, WESSELLS H, CHANCELLOR M B, et al.Urologic complications of diabetes[J].Diabetes Care, 2005, 28 (1) :177-185.[2]BROWN J S, WESSELLS H, CHANCELLOR M B, et al.Urologic complications of diabetes[J].Diabetes Care, 2005, 28 (1) :177-185.
[3]	[3]DANESHGARI F, LIU G, IMREY P B.Time dependent changes in diabetic cystopathy in rats include compensated and decompensated bladder function[J].J Urol, 2006, 176 (1) :380-386.[3]DANESHGARI F, LIU G, IMREY P B.Time dependent changes in diabetic cystopathy in rats include compensated and decompensated bladder function[J].J Urol, 2006, 176 (1) :380-386.
[4]	[4]WANG C C, NAGATOMI J, TOOSI K K, et al.Diabetes-induced alternations in biomechanical properties of the urinary bladder wall in rats[J].Urology, 2009, 73 (4) :911-915.[4]WANG C C, NAGATOMI J, TOOSI K K, et al.Diabetes-induced alternations in biomechanical properties of the urinary bladder wall in rats[J].Urology, 2009, 73 (4) :911-915.
[5]	[5] GOMEZ C S, KANAGARAJAH P, GOUSSE A E.Bladder dys-function in patients with diabetes[J].Curr Urol Rep, 2011, 12 (6) :419-426.[5]GOMEZ C S, KANAGARAJAH P, GOUSSE A E.Bladder dys-function in patients with diabetes[J].Curr Urol Rep, 2011, 12 (6) :419-426.
[6]	[6]ALIMOHAMMADI S, HOBBENAGHI R, JAVANBAKHT J, et al.Protective and antidiabetic effects of extract from Nigella sativa on blood glucose concentrations against streptozotocin (STZ) -induced diabetic in rats:an experimental study with histopathological evaluation[J].Diagn Pathol, 2013, 15 (8) :137-144.[6]ALIMOHAMMADI S, HOBBENAGHI R, JAVANBAKHT J, et al.Protective and antidiabetic effects of extract from Nigella sativa on blood glucose concentrations against streptozotocin (STZ) -induced diabetic in rats:an experimental study with histopathological evaluation[J].Diagn Pathol, 2013, 15 (8) :137-144.
[7]	[7]CHAUDHRY Z Z, MORRIS D L, MOSS D R, et al.Streptozotocin is equally diabetogenic whether administered to fed or fasted mice[J].Lab Anim, 2013, 47 (4) :257-265.[7]CHAUDHRY Z Z, MORRIS D L, MOSS D R, et al.Streptozotocin is equally diabetogenic whether administered to fed or fasted mice[J].Lab Anim, 2013, 47 (4) :257-265.
[8]孙丽华, 崔海峰, 孙明杰, 等.链脲佐菌素制备糖尿病大鼠模型探讨[J].中国实验动物学报, 2012, 12 (6) :15-19.[8]孙丽华, 崔海峰, 孙明杰, 等.链脲佐菌素制备糖尿病大鼠模型探讨[J].中国实验动物学报, 2012, 12 (6) :15-19.
[9]孙泽禹, 侯建全, 温瑞改.早期糖尿病大鼠膀胱的动力学及病理组织学[J].苏州大学学报 (医学版) , 2008, 28 (3) :364-368.[9]孙泽禹, 侯建全, 温瑞改.早期糖尿病大鼠膀胱的动力学及病理组织学[J].苏州大学学报 (医学版) , 2008, 28 (3) :364-368.
[10]罗广承, 何志华, 罗建珍, 等.构建糖尿病膀胱病豚鼠模型及其尿动力学评价[J].中国工程组织研究, 2014, 18 (7) :1063-1067.[10]罗广承, 何志华, 罗建珍, 等.构建糖尿病膀胱病豚鼠模型及其尿动力学评价[J].中国工程组织研究, 2014, 18 (7) :1063-1067.
[11]陈朝江, 周兴, 孔桃红, 等.链脲佐菌素复制大鼠糖尿病神经源性膀胱膀胱模型[J].中国现代医学杂志, 2013, 23 (32) :18-21.[11]陈朝江, 周兴, 孔桃红, 等.链脲佐菌素复制大鼠糖尿病神经源性膀胱膀胱模型[J].中国现代医学杂志, 2013, 23 (32) :18-21.
[12]韩宾林, 王东文, 双卫兵.2型糖尿病大鼠不同时期膀胱收缩力动态变化的实验研究[J].中国药物与临床, 2013, 13 (5) :549-551.[12]韩宾林, 王东文, 双卫兵.2型糖尿病大鼠不同时期膀胱收缩力动态变化的实验研究[J].中国药物与临床, 2013, 13 (5) :549-551.
[13]王东文, 双卫兵, 吴博威.糖尿病膀胱尿动力学及逼尿肌功能改变的相关性研究[J].中华泌尿外科杂志, 2006, 27 (5) :314-317.[13]王东文, 双卫兵, 吴博威.糖尿病膀胱尿动力学及逼尿肌功能改变的相关性研究[J].中华泌尿外科杂志, 2006, 27 (5) :314-317.
[14]	[14]BANSAL R, AGARWAL M M, MODI M, et al.Urodynamic profile of diabetic patients with lower urinary tract symptoms:association of diabetic cystopathy with autonomic and peripheral neuropathy[J].Urology, 2011, 77 (3) :699-705.[14]BANSAL R, AGARWAL M M, MODI M, et al.Urodynamic profile of diabetic patients with lower urinary tract symptoms:association of diabetic cystopathy with autonomic and peripheral neuropathy[J].Urology, 2011, 77 (3) :699-705.
[15]	[15]LI W J, OH S J.Diabetic cystopathy is associated with PARP/JNK/mitochondrial apoptotic pathway-mediated bladder apoptosis[J].Neurourol Urodyn, 2010, 29 (7) :1332-1337.[15]LI W J, OH S J.Diabetic cystopathy is associated with PARP/JNK/mitochondrial apoptotic pathway-mediated bladder apoptosis[J].Neurourol Urodyn, 2010, 29 (7) :1332-1337.
[16]	[16]LIN T L, CHEN G D, CHEN Y C, et al.Aging and recurrent urinary tract infections are associated with bladder dysfunction in type 2 diabetes[J].Taiwan J Obstet Gynecol, 2012, 51 (3) :381-386.[16]LIN T L, CHEN G D, CHEN Y C, et al.Aging and recurrent urinary tract infections are associated with bladder dysfunction in type 2 diabetes[J].Taiwan J Obstet Gynecol, 2012, 51 (3) :381-386.
[17]	[17]GOMEZ C S, KANAGARAJAH P, GOUSSE A E.Bladder dysfunction in patients with diabetes[J].Curr Urol Rep, 2011, 12 (6) :419-426.[17]GOMEZ C S, KANAGARAJAH P, GOUSSE A E.Bladder dysfunction in patients with diabetes[J].Curr Urol Rep, 2011, 12 (6) :419-426.
[18]	[18]LEE W C, WU H P, TAI T Y, et al.Investigation of urodynamic characteristics and bladder sensory function in the early stages of diabetic bladder dysfunction in women with type 2 diabetes[J].J Urol, 2009, 181 (1) :198-203.[18]LEE W C, WU H P, TAI T Y, et al.Investigation of urodynamic characteristics and bladder sensory function in the early stages of diabetic bladder dysfunction in women with type 2 diabetes[J].J Urol, 2009, 181 (1) :198-203.
[19]	[19]GAUDREAU G A, PLOURDE V.Involvement of Nmethyl-D-as-partate (NMDA) receptors in a rat model of visceral hypersensitivity[J].Behav Brain Res, 2004, 150 (1-2) :185-189.[19]GAUDREAU G A, PLOURDE V.Involvement of Nmethyl-D-as-partate (NMDA) receptors in a rat model of visceral hypersensitivity[J].Behav Brain Res, 2004, 150 (1-2) :185-189.
[20]高宏飞, 王东文.2型糖尿病膀胱重构的受体机制研究[J].中西医结合心脑血病杂志, 2013, 11 (6) :716-718.[20]高宏飞, 王东文.2型糖尿病膀胱重构的受体机制研究[J].中西医结合心脑血病杂志, 2013, 11 (6) :716-718.

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