HPLC指纹图谱联合化学计量学在不同产地云木香药材鉴别中的应用
Identification of Radix Saussureae lappae from Different Habitats by HPLC Fingerprints Combined with Chemometrics
-
摘要: 目的 应用高效液相色谱法检测云木香药材指纹图谱, 采用多种化学计量学方法分析, 以鉴别不同产地云木香药材.方法 采用反相高效液相色谱法, 乙腈-水为流动相梯度程序洗脱, 流速1.0 m L/min, 检测波长254 nm, 检测云木香药材的HPLC图谱;结合相似度计算、系统聚类分析、主成分分析等方法分析指纹图谱, 对不同产地云木香进行鉴别.结果 该方法可使云木香中各成分得到较好的分离;根据检测结果确定了18个共有指纹峰, 建立了云木香药材指纹图谱的共有模式, 15个不同来源的云木香有一定相似度;但不同产地云木香药材样品的指纹图谱通过化学计量学分析, 发现存在较大差异.结论 该HPLC指纹图谱重复性好, 特异性高.HPLC指纹图谱联合化学计量学可鉴别不同产地云木香药材, 为云木香药材质量控制提供依据, 并为云木香药材资源合理开发利用提供参考.Abstract: Objectives To identify Radix Saussureae lappae from different habitats by application of HPLC fingerprints and chemometrics. Methods By using reversed phase high performance liquid chromatography (HPLC) , the analysis was carried out with the mobile phases of Acetonitrile and water by gradient elution. The flow rate was 1.0 m L/min, and the UV detection wave length was 254 nm. Similarity calculation, principal component analysis and hierarchical cluster analysis were applied to read HPLC fingerprints, then to identify of Radix Saussureae lappae from different habitats. Results The method could be used to separate the components of Radix Saussureae lappae. According to the test results, 18 common fingerprint peaks were assigned, and a common HPLC fingerprint pattern of Radix Saussureae lappae was established, and the similarity of Radix Saussureae lappae from 15 different sources was high. But there were significant differences in the fingerprint patterns of samples from different habitats. Conclusion With excellent specificity and reproducibility, the fingerprinting method provides a feasible method for identification of Radix Saussureae lappae from different habitats, and can provide references for Radix Saussureae lappae quality control, and provide references for resources reasonable development and utilization of Radix Saussureae lappae.
-
Key words:
- Radix Saussureae lappae /
- HPLC /
- Fingerprints /
- Chemometrics
-
[1]中国科学院中国植物志编辑委员会.中国植物志[M]北京:科学出版社, 2004:058. [2] 国家药典委员会.中华人民共和国药典:2010年版一部[S].北京:中国医药科技出版社, 2010:57-58. [3]江苏新医学院.中药大辞典:上册[M].上海:上海科学技术出版社, 1979:353. [4]梁凤书, 高宏光.道地药材云木香不同产地的质量研究.云南中医中药杂志, 2005, 26 (6) :23. [5] 魏·吴普, 清·孙星衍, 孙冯冀辑.神农本草经[M].北京:人民卫生出版社, 1997:186. [6]王永兵, 王强, 毛福林, 等.木香的药效学研究[J].中国药科大学学报, 2001, 32 (2) :146-148. [7] [7]SINGH I P, TALWAR K K, ARORA J K, et a1.A biologically active guaianolide from Saussurea lappa[J].Phytochemistry, 1992, 31 (7) :2529-2531. [8] [8]TALWAR K K, SINGH I P, KALSI P S.A sesquiterpenoid with plant growth regulatory activity from Saussurea lappa[J].Phytochemistry, 1992, 31 (1) :336-338. [9]林明峡.木香的药理及临床研究概况[J].中医药信息, 2005, 22 (3) :18-19. [10]杨辉, 谢金伦, 孙汉董.云木香化学成分及药理作用研究概况[J].天然产物研究与开发, 1998, 10 (2) :90-98. [11]徐宇, 方鲁延, 谈红, 等.HPLC法测定木香中去氢木香内酯的含量[J].中草药, 2004, 35 (12) :1416-1417. [12]李力, 张振秋, 王冰, 等.木香药材HPLC指纹图谱的研究[J].中成药, 2011, 33 (6) :925-928. [13]梁晟, 梁逸曾, 李雅文, 等.木香挥发性化学成分的气相色谱-质谱分析[J].广州化学, 2007, 32 (4) :12. [14]侯鹏飞, 陈文星, 赵新慧, 等.木香挥发性成分气质联用分析及其抑制血小板聚集作用的研究[J].中国实验方剂学杂志, 2008, 14 (7) :26. [15]张兰胜, 杨志勇, 董光平, 等.云木香挥发油化学成分的研究[J].大理学院学报 (综合版) , 2007, 6 (12) :9. [16]邱琴, 崔兆杰, 刘廷礼, 等.GC/MS法测定木香挥发油化学成分[J].理化检验化学分册, 2001, 37 (8) :346. [17]陈飞龙, 谭晓梅, 汤庆发, 等.不同产地木香挥发油成分的GC-MS分析比较[J].中国药房, 2011, 22 (23) :2187-2189. [18]王瑾, 胡臻华.中药指纹图谱的研究概述[J].解放军药学学报, 2004, 20 (3) :214-216. [19]侯小平, 何新新, 苏薇薇.中药指纹图谱质量控制技术[J].中药材, 2002, 24 (5) :370-371. -
点击查看大图
计量
- 文章访问数: 1365
- HTML全文浏览量: 499
- PDF下载量: 182
- 被引次数: 0
下载:
